3 pages (750 words)
, Term Paper
This method was introduced in 1967 and has since become a popular PAGE process. (Shapiro et al, 1967) SDS PAGE sample contains sodium dodecyl sulphate/lauryl sulfate, ?-mercaptoethanol (BME), bromophenyl blue, glycerol, and Tris-glycine at pH 6.8. SDS removes secondary and tertiary protein structures and maintains the proteins as polypeptide chains, it also gives negative charges to protein molecules, ?