quora
Nobody downloaded yet

Production of tpa using eukaryotic n prokaryotic cells - Essay Example

Comments (0) Cite this document
Summary
Mammalian cell culture has a wide application in biotechnology where they have been utilized for the production of vaccines, proteins, hormones, antibodies and enzymes. The challenge with…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER97.5% of users find it useful
Production of tpa using eukaryotic n prokaryotic cells
Read TextPreview

Extract of sample
"Production of tpa using eukaryotic n prokaryotic cells"

Download file to see previous pages coli (prokaryotic). This is because of the high cost of the purification scheme required on the tPA produced from E. coli. That from the mammalian cells does not require lots of purifications because the cell environment and content is similar to that of the body cells (in vitro). tPA produced in E. coli required more purification to acquire the required state because it is composed of many components that are naturally not found with tPA in vitro conditions. The contents produced together with tPA in mammalian cells are similar to those in vitro hence are not foreign materials resulting to a less purification process. The E. coli based production process utilizes approximately sixteen purification steps (downstream) as compared to the five purification steps in CHO. Since the purification process in mammalian cells is not that complex less operating labour and supervision is required compared to the E. coli based. The CHO process utilizes nine percent of its total cost on operating labour while E. coli based utilizes twenty two percent. Also, the cost of acquiring the different purification units required for the many purification stages is higher in the E. coli based. Mammalian based process utilizes only twenty five percent of the total production cost on recovery (purification) compare to eighty eight percent on E. coli based. For the solubilization and renaturation step in the E. coli based production, approximately five tonnes of urea and twenty-six tonnes of guanidine are required.
The bacteria lack efficient secretion mechanisms for tPA, therefore, the proteins are deposited as inclusion bodies. For the recovery of the proteins, a renaturation procedure is required. Since the proteins in the bacteria are produced in low concentrations (about 2.4 mg per litre) the practicability of a renaturation based production process is limited. The E. coli based production does yield to larger quantities of products that ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Production of tpa using eukaryotic n prokaryotic cells Essay”, n.d.)
Production of tpa using eukaryotic n prokaryotic cells Essay. Retrieved from https://studentshare.org/biology/1633911-production-of-tpa-using-eukaryotic-n-prokaryotic-cells
(Production of Tpa Using Eukaryotic N Prokaryotic Cells Essay)
Production of Tpa Using Eukaryotic N Prokaryotic Cells Essay. https://studentshare.org/biology/1633911-production-of-tpa-using-eukaryotic-n-prokaryotic-cells.
“Production of Tpa Using Eukaryotic N Prokaryotic Cells Essay”, n.d. https://studentshare.org/biology/1633911-production-of-tpa-using-eukaryotic-n-prokaryotic-cells.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Eukaryotic and Prokaryotic cells
Light Microscope or Electron Microscopes are used to view these tiny cells. These cells are of many different types, which make the appearance and characteristics distinct from each other. Eukaryotic and Prokaryotic cells are two such cells which have DNA as their generic material.
2 Pages(500 words)Essay
Detecting Circulating Tumor Cells using Flow Cytometry
The system has a disposable chip. This chip checks for cross contamination collect analyzed sample and to freely measurement. CTC is salient biomarkers for so many cancers. There are many systems for enumeration based on either EpCAM/CD326 which express tumor cell before microscope or RT-PCR.
6 Pages(1500 words)Essay
The Essential Features of Prokaryotic Replication
These less complex cells, which include Bacteria and Archaea, are called prokaryotes or prokaryotic cells. Some microorganisms are prokaryotic, some are eukaryotic, and some are not cells at all. For maintenance of the progeny, reproduction of all cells is necessary.
4 Pages(1000 words)Essay
Production Planning using ERP
According to Waldner (1992) MRP or material requirements planning system is tool that is based on the inventory control and production planning system and is utilized to handle
4 Pages(1000 words)Essay
Prokaryotic and Eukaryotic Cells
4). Prokaryotic cells are classified as either bacteria or archaea. Another major difference is that eukaryotic cells have most DNA strands contained in a nucleus, whereas prokaryotic cells have circular DNA and do not have a nucleus (Fancher). Eukaryotic cells also have organelles, which are specialized structures that transfer energy that prokaryotic cells do not have (Krough, Ch.
2 Pages(500 words)Essay
Correcting Sickle Cell Disease using Stem Cells
The disease manifests after 4 months of age, and its symptoms include painful episodes that last from hours to days, breathlessness, delayed growth, fatigue, fever, rapid heart rate,
1 Pages(250 words)Essay
Exam 2
e various researches done on evolution of cells by different scientists each research is trying to elaborate himself by really looking in the weakness of the opponent as the weakness from you will be used as the improvement of the other. However, with criticism many theories
4 Pages(1000 words)Essay
1. Describe briefly the roles of the different parts of the eukaryotic cells endomembrane system in the production and excretion of proteins
The rough endoplasmic reticulum avails adequate surface area meant to accommodate extra ribosomes, which are sites for protein synthesis (Gupta, 2009). Proteins
1 Pages(250 words)Essay
Within almost all eukaryotic cells the endoplasmic reticulum, Golgi complex and endosomes can be considered as a system, cooperating to deliver secerted material to the cell surface
These membranes perform different functions such as dividing the cell into structural and functional components or organelles (Cooper, 2000). For instance, in the eukaryotes, the compartment in the endomembrane system is composed of
10 Pages(2500 words)Essay
Production of tpa using eukaryotic n prokaryotic cells
Due to their high demand, drugs are scarce leading to sky rocketing of prices thus raising questions concerning the cost of producing drugs. Production of newly discovered drugs is expensive and requires huge sums of money during packaging and marketing. During
2 Pages(500 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic Production of tpa using eukaryotic n prokaryotic cells for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us