Nobody downloaded yet

CRISPR/CAS9 in Toxoplasma gondii - Article Example

Comments (0) Cite this document
CRISPR/Cas 9 system is widely used for genome engineering in eukaryotic organisms, because it helps in the rapid generation of the cell lines with the modified alleles. CRISPR along with Cas9 acts as an auto immune system to the body, with the Cas proteins converting the foreign…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER94.4% of users find it useful
CRISPR/CAS9 in Toxoplasma gondii
Read TextPreview

Extract of sample "CRISPR/CAS9 in Toxoplasma gondii"

Download file to see previous pages CRISPR- Cas 9 system is used for the production of recombinant proteins in Humans, Mice, Caenorhabditis elegans, Saccharomyces cerevisiae, Zebra Fish, Drosophila melanogaster, Sorghum, Wheat and Arabidopsis. The efficiency and the precision of genome editing by CRISPR/Cas 9 system in the tested organisms are very high. Thus, the genome editing of Toxoplasma Gondii using CRISPR/Cas9 system can be performed.
CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats, was identified around 1980s in the bacteria. (Barrangou & Oost, 2012). CRISPR helps to turn on the active components, which are present in between the spacer DNA, as repeats. The CRISPR fragments get easily incorporated into the plasmids, viruses and other genomes. These spacer elements can be used to create small silencing RNA molecules (siRNAs) to prevent virus replication. Similarly, the bacteria can be made resistant to virus by introducing these small spacer elements. Thus, CRISPR plays an important role in the functioning of the immune system, and it is generally described as genome immune system. (Shapiro, 2011).
CRISPR spacers get associated with many Cas proteins and acts as auto immune system in the body. Six core Cas proteins are identified so far and the total Cas family count is expected to be up to 45. In a microbial genome, there will be more than one CRISPR- Cas locus. Cas 1 and Cas 2 proteins are considered as the important markers for CRISPR loci, with Cas 1 being the most conserved Cas protein and is present in all CRISPR loci. (Gardner & Olson, 2012). CAS 1 and Cas 2 are metal dependent as well as DNA specific, and so are highly conserved endonucleases. They require either manganese or magnesium ion to function. In addition, Cas 1 and CAS 2 require one more Cas protein to function. As far as other proteins are concerned, Cas3 protein contains ATP- dependent helicase and Mg- dependent ssDNA nuclease. (Gardner & Olson, ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“CRISPR/CAS9 in Toxoplasma gondii Article Example | Topics and Well Written Essays - 3000 words”, n.d.)
CRISPR/CAS9 in Toxoplasma gondii Article Example | Topics and Well Written Essays - 3000 words. Retrieved from
(CRISPR/CAS9 in Toxoplasma Gondii Article Example | Topics and Well Written Essays - 3000 Words)
CRISPR/CAS9 in Toxoplasma Gondii Article Example | Topics and Well Written Essays - 3000 Words.
“CRISPR/CAS9 in Toxoplasma Gondii Article Example | Topics and Well Written Essays - 3000 Words”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document


Protective immune response against Toxoplasma gondii elicited by recombinant DNA vaccines

...? Protective immune response against Toxoplasma gondii elicited by recombinant DNA vaccines Introduction The high incidence or fatal damage rendered by T. gondii infection evidently indicates the urge for the development of a vaccine. The development of effective DNA vaccines against T. gondii can help mitigate and control the spread of toxoplasmosis, a move that is critical to both human health and the farming industry. Immunization with DNA vaccines can stimulate humoral and cell-mediated immune response, all of which are significant in conferring immunity to Toxoplasma gondii (Dubey 2008, p.467). Statistics indicate that, in the UK,...
11 Pages(2750 words)Essay

Possibility article

...slaves. Thus, there is what you call Toxoplasmosa Gondii parasite that seems to give the same effect that can lead to such terrifying holocaust. “Human and rat brains share the same basic anatomy and neurotransmitterrs, which is why rats are common in human drug expirements. The possibility of viral mutation brings the possibility that the virus could adapt to the human anatomy and have us behave in the same way as the rats, bringing a loss of self preservation and willpower” (Drew Parks, 2008). Deliberately, such parasite extends great posibility of creating zombie-like human slaves. It is widely known that whenever a drug or a study is conducted in a rat, the rat acts wildy and out of control due to the adverse...
1 Pages(250 words)Essay

Seroconversion and Infectious Mononucleosis

..., ensuing B-lymphocyte malignancies or B-cell lymphomas. Fever is due to immune response where cytokine liberation is followed by B-lymphocyte attack by EBV. Lymphocytosis and pharyngitis occur due proliferation of EBV-infected B lymphocytes (Higgins et al., 2007; Dorothy et al., 2006). Conclusion The virus possesses prolonged latency period, and displays symptoms like fever and sore throat which could be confusing with the common cold symptoms and also infection by other organisms such as Toxoplasma gondii. It is therefore essential to have a undergo differential diagnosis to confirm the acute infectious mononucleosis especially in immuno-compromised individuals. The present article is an effort to...
5 Pages(1000 words)Essay

The use of the internet among the youth fosters social cohesiveness

4 Pages(1000 words)Essay


...?TOXOPLASNOSIS TOXOPLASNOSIS In the world, there has been the emergence of various infections. Most of these infections have become a serious area of heath care concern. However, there has been little interest on some of the infections. Most of these infections are caused by parasites. The most common infection that appears forgotten is toxoplasmosis. The infection has been popular in all human population (David et al., n.d.). The infection affects all section of the society. A reflection on toxoplasmosis helps in understanding its aetiology, epidemiology, signs and symptoms, prognosis, and treatment. The disease is caused by the protozoan parasites known as Toxoplasma gondii (Cornell University College...
3 Pages(750 words)Essay

Symptomatic Toxoplasmosis

...rise in the affected humans stated that this rise could result in an epidemic and it should be controlled from now on to avoid future consequences. The table shows that Mrs H.D Hardwick is right about the disease as the standard deviation value has shown a rise all of a sudden after two years in which there was reportedly no case of human disease concerning toxoplasmosis. Thus if not taken care of now the disease would further spread in other human beings and would also affect the marine life heavily. Q3. If table 1 is examined it is clearly known that there is a huge difference in the mortality rate that is occurring due to the Toxoplasma gondii associated protozoal meningoencephalitis in the...
4 Pages(1000 words)Research Paper


...TOXOPLASMOSIS Toxoplasma gondii is a major disease causing protozoa which infects basically the blood and the tissues. Toxoplasma gondii can cross the placental barrier and infect the fetus leading to the development of the condition which is referred to as congenital toxoplasmosis. Toxoplasmosis is a condition which occurs all across the world. In the United States, it was analyzed that 5 to 10% of people in the different areas of the country had antibodies against the organism. The protozoa itself is also found in many birds as well as animals but the cats and the felines are the only animals where the parasite can replicate and lead to disease. It was found in a...
5 Pages(1250 words)Term Paper

Clinical immunology case study

...-induced IgAD can be verified by the return of IgA to normal levels once intake of the culprit drug is stopped (Dolina, 2011). In addition, infections like Rubella, Cytomegaloviruses, Toxoplasma gondii, and Epstein-Barr virus may also cause transient IgAD. The most effective management of IgAD in this case will be the resolution of the infection (Dolina, 2011). Finally, bone marrow transplantation from an IgA-deficient donor may also cause IgAD to the recipient. History of bone marrow transplantation and IgAD in the donor after the said surgical procedure point to acquired IgAD. It is thus important to thoroughly screen the donors before proceeding with the transplant (Dolina, 2011). Question 6:...
8 Pages(2000 words)Essay

Protocol Bacteriology(microbial disease and immunology)

...1. Differential diagnosis s of possible bacteria) You should include at least 6 most relevant bacteria that can cause signs and symptoms given in the case study and rationale for including each of them (1 sentence maximum for each). a. Mycobacterium tuberculosis ­– It is the causative agent of tuberculosis, which can be a multi-organ disease. b. Chlamydia pneumoniae – The biphasic presentation, as well as the fever, cough and eye discharge symptoms may indicate concurrent pneumonia and eye infection by the organism. c. Toxoplasma gondii – A protozoan, which when infects humans, usually the immunocompromised one, causes a variety of symptoms, including malaise, fever, cough, and eye symptoms. d....
1 Pages(250 words)Assignment

Is human genome editing inevitable

...Is human genome editing inevitable? Throughout the world, millions of people are affected because of mutations of genes in human genome and the resultant rare health disorders. Scientists of Genetic Engineering Technology have been carrying out extensive researches with the aim of perfecting gene therapies for treating various genetically-affected medical conditions. Genome editing is a type of genetic engineering that purports to insert, replace, or remove a genome through artificially-engineered nucleases or molecular scissors. Widespread adoption of this process has recently fueled a technique called CRISPR-Cas9 - a new approach that generates RNA-guided nucleases with customizable specificities....
5 Pages(1250 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Article on topic CRISPR/CAS9 in Toxoplasma gondii for FREE!

Contact Us