Nobody downloaded yet

CRISPR/CAS9 in Toxoplasma gondii - Article Example

Comments (0) Cite this document
CRISPR/Cas 9 system is widely used for genome engineering in eukaryotic organisms, because it helps in the rapid generation of the cell lines with the modified alleles. CRISPR along with Cas9 acts as an auto immune system to the body, with the Cas proteins converting the foreign…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.4% of users find it useful
CRISPR/CAS9 in Toxoplasma gondii
Read TextPreview

Extract of sample
"CRISPR/CAS9 in Toxoplasma gondii"

Download file to see previous pages CRISPR- Cas 9 system is used for the production of recombinant proteins in Humans, Mice, Caenorhabditis elegans, Saccharomyces cerevisiae, Zebra Fish, Drosophila melanogaster, Sorghum, Wheat and Arabidopsis. The efficiency and the precision of genome editing by CRISPR/Cas 9 system in the tested organisms are very high. Thus, the genome editing of Toxoplasma Gondii using CRISPR/Cas9 system can be performed.
CRISPR, which stands for Clustered Regularly Interspaced Short Palindromic Repeats, was identified around 1980s in the bacteria. (Barrangou & Oost, 2012). CRISPR helps to turn on the active components, which are present in between the spacer DNA, as repeats. The CRISPR fragments get easily incorporated into the plasmids, viruses and other genomes. These spacer elements can be used to create small silencing RNA molecules (siRNAs) to prevent virus replication. Similarly, the bacteria can be made resistant to virus by introducing these small spacer elements. Thus, CRISPR plays an important role in the functioning of the immune system, and it is generally described as genome immune system. (Shapiro, 2011).
CRISPR spacers get associated with many Cas proteins and acts as auto immune system in the body. Six core Cas proteins are identified so far and the total Cas family count is expected to be up to 45. In a microbial genome, there will be more than one CRISPR- Cas locus. Cas 1 and Cas 2 proteins are considered as the important markers for CRISPR loci, with Cas 1 being the most conserved Cas protein and is present in all CRISPR loci. (Gardner & Olson, 2012). CAS 1 and Cas 2 are metal dependent as well as DNA specific, and so are highly conserved endonucleases. They require either manganese or magnesium ion to function. In addition, Cas 1 and CAS 2 require one more Cas protein to function. As far as other proteins are concerned, Cas3 protein contains ATP- dependent helicase and Mg- dependent ssDNA nuclease. (Gardner & Olson, ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“CRISPR/CAS9 in Toxoplasma gondii Article Example | Topics and Well Written Essays - 3000 words”, n.d.)
CRISPR/CAS9 in Toxoplasma gondii Article Example | Topics and Well Written Essays - 3000 words. Retrieved from https://studentshare.org/biology/1637237-crisprcas9-in-toxoplasma-gondii
(CRISPR/CAS9 in Toxoplasma Gondii Article Example | Topics and Well Written Essays - 3000 Words)
CRISPR/CAS9 in Toxoplasma Gondii Article Example | Topics and Well Written Essays - 3000 Words. https://studentshare.org/biology/1637237-crisprcas9-in-toxoplasma-gondii.
“CRISPR/CAS9 in Toxoplasma Gondii Article Example | Topics and Well Written Essays - 3000 Words”, n.d. https://studentshare.org/biology/1637237-crisprcas9-in-toxoplasma-gondii.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Most of these infections are caused by parasites. The most common infection that appears forgotten is toxoplasmosis. The infection has been popular in all human population (David et al., n.d.). The infection affects all section of the society. A reflection on toxoplasmosis helps in understanding its aetiology, epidemiology, signs and symptoms, prognosis, and treatment.
3 Pages(750 words)Essay
The Use of Humor in I Claudius
Humor and wit from this renowned tale, I, Claudius by Robert Graves feel in a way that completely enthralls the reader. The story captured during the ancient rule of Augustus, Tiberius, Caligula, and Claudius provides compelling scenes in which the characters feel their listeners with unmistakable bouts of wit.
2 Pages(500 words)Essay
Possibility article
In 2002, the horror film written and directed by Paul W.S. Anderson made a huge success in the big screen which further opened avenues for more successful sequels of the movie. The film progressed following the life of Alice, an amnesiac heroine, of whom one day unknowingly woke up in the band of a secret underground facility of the Umbrella Corporation.
1 Pages(250 words)Essay
Protective immune response against Toxoplasma gondii elicited by recombinant DNA vaccines
The development of effective DNA vaccines against T. gondii can help mitigate and control the spread of toxoplasmosis, a move that is critical to both human health and the farming industry. Immunization with DNA vaccines can stimulate humoral and cell-mediated immune response, all of which are significant in conferring immunity to Toxoplasma gondii (Dubey 2008, p.467).
11 Pages(2750 words)Essay
A Brief History of Environmental Movements
It evolved from local membership to professional membership consisting of specialists. At the time, about ten organizations had been registered as environmental parties where its members increased
2 Pages(500 words)Assignment
Symptomatic Toxoplasmosis
Thus if not taken care of now the disease would further spread in other human beings and would also affect the marine life heavily. Q3. If table 1 is examined it is clearly known that there is a huge difference in the mortality rate that is occurring due to the Toxoplasma gondii associated protozoal meningoencephalitis in the population A and population B.
4 Pages(1000 words)Research Paper
Clinical immunology case study
Question 4: The patient is a 50 year old male, with a chief complaint of recurrent respiratory infection since he was a teenager, resulting to annual antibiotics use. No family history of respiratory illness, and no personal history of
8 Pages(2000 words)Essay
In the United States, it was analyzed that 5 to 10% of people in the different areas of the country had antibodies against the organism. The protozoa itself is also found in many birds as well as animals but the cats and the
5 Pages(1250 words)Term Paper
Write a essay about those questions
Congenital syphilis is also possible during pregnancy where an infected mother may pass the bacteria to the unborn baby. Syphilis
6 Pages(1500 words)Essay
Protocol Bacteriology(microbial disease and immunology)
There is no need to give the details of the methods but you must include the nature and rationale for each test and the order in which they should be done (if appropriate, you should also give the expected results for each test.) From respiratory secretions of an infected
1 Pages(250 words)Assignment
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Article on topic CRISPR/CAS9 in Toxoplasma gondii for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us