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Breast Cancer - Lab Report Example

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The aim of the paper “Breast Cancer” is to ascertain the level of staining that could be observed between the fifth and sixth cell lines. The sixth cell line which was blocked with carbon free blockin solution,is seen to have an intense staining as compared to the fifth cell line…
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Breast Cancer
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"Breast Cancer"

Download file to see previous pages Acccording to studies,it is known that glycosylation is more pronounced in the breast cancer cells (Hakomori 1985). The reason for this is because of the increase in tne oligosaccharide structures (Hudis 2007).Such alterations might be identified through lectins which are the carbohydrate-binding proteins  an they have a very high specificity for the saccharides(Brooks and harris 2006).The lectins normally function as  the data mediators in the biological systems.In this case they interact with the glycoproteins glycolipids as well as the oligosaccharides(Nilsson 2007).They also bind specifically to the carbohydrate epitopes(Staffieri et al. 2012).The immunofluorescence method as well as the direct lectins cytochemistry  with the use of the  FITC-labeled lectins enables the visualization of the glycosylation’s impact on the therapies which targets the surface of the cells that bind to theHER2 receptors (Gabius 2009.The results from the histograms above (fig 1), indicates the reason why the glycosylation’s intensity is higher in the sixth cell line when compared to that of the fifth cell line because of the different blocking agents used. In summary, further study needs to be done to clearly understand why there was a difference in the intensity of this two cell lines. It could be due to the above three mentioned reasons. Unfortunately, this study could not ascertain this.The reasons that could have resulted to the difference in the intesity could be due; firstly,the BSA could be a better blocking agent. ...Download file to see next pagesRead More
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