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Further, the use of Mosel agar also helped to isolate the bacteria. This lab report consists of the results obtained for the isolation process.
In this lab, the main objective was to isolateBacillus subtilis which is a gram-positive bacteria. The bacteria exist naturally in soil or vegetation; and within a mesophilic temperature range of 25-35 degrees Celsius. The bacterium has rod-shaped cells hence leading to the name bacillus. Usually, it leaves in harsh conditions, a situation leading to the formation of endospores which are stress resistant (Boone et al, 2001). The characteristics of the bacteria which allows for the uptake of of external DNA also provided a hint in isolating the microbe from its culture. The characteristics of the organism makes it significant to make use of gram staining and mosel agar to help in the isolation process. The following is the image showing Bacillus subtilis colony
The processed sample of the bacterium was treated through suspending it in a physiological saline for 15 minutes. The temperature for the treatment process was kept at 100 degrees Celsius to ensure that all the vegetative forms involved were all killed. The culture was then isolated in a nutrient agar. Gram staining method was then applied to guide in genus identification (Dworkin & Fulkow, 2006). The shape of the rods and extent was then examined from gram stains. Further , Mosel agar was used in isolating the individual colony. Glucose nitrate was also helpful idenfying the associated growth. The color of the growth was examined and recorded.
On performing gram staining, there was formation of slender rods and the realization of gram positive nature, to represent Bacillus subtilis. This helped in isolating the microbe from B. Cereus which assumed the shape of thick swollen rods.
The treatment under 100 degrees Celsius was useful in isolating the species from its vegetative forms since the bacteria itself
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Nonetheless, starvation did not contribute many deaths since citizens engaged in other means of earning their livelihood. Sommerville noted that England suffered from the effects of the Great Starvation that affected the country between 1315 and 1317 although there is no enumeration of people who died due to starvation.
The secondary purpose was to differentiate the general selective, purpose, and differential media, and give illustrations in the form of examples, and describe the conditions under which they are useful. In enzymes experiment, its primary objective was to assay the culture of bacteria to find out if certain enzymes are developed or produced.
It connotes the rapidity with which the disease strikes its victims. Plague is endemic among rodents, particularly in central Asia, but does not cause many fatalities. However, if it crosses the biological barrier to attack other species, it can prove deadly. The disease-causing pathogen is the bacillus Yersinia pestis, which was identified by the French bacteriologist Alexandre Yersin only in 1894.
According to the studies made by U.S. Centers for Disease Control and Prevention, an estimate of 336,000 cases had been diagnosed positively with C. difficile infection in the year 2009. The 2009 report has shown an increase in the C. difficile spread as compared to the 139,000 diagnosed cases in the year 2000.
In terms of physiology, they are basically autotrophic with all their materials from inorganic sources and photosynthetic producing complex carbon compounds from CO2 and light energy. Some algae are secondarily heterotrophic but retaining fundamental
Introduction The term “algae” a Latin term for seaweed, is a broad description to include a variety of phyla that fit the general description in terms of morphology and general physiology. As very simple organisms, they have no roots, stems and leaves.
Under favorable conditions, they multiply very rapidly, forming colonies of millions of organisms within a limited space. Steyn states that bacterial characteristics are among the most diverse in any life
One good news is that it is not impossible to manage and cure a person from infection by anthrax. Vaccines are available and, provided exposure to anthrax has been detected early enough, it is possible to conduct
Direct contact with the secretion of patient can also transmit diphtheria to others. Primary symptoms of the disease are fever, problematic breathing, sore throat, malaise and neck enlargement. Antibiotics and antitoxins are used for treating the patient with diphtheria.
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