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Control by Heat and UV - Lab Report Example

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These include oxygen, temperature, and light. Different bacterial species have different requirements for these environmental factors. The requirements vary according to the habitat of the…
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Control by Heat and UV
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Download file to see previous pages Some bacteria though have developed adaptive measures to sustain high-temperature exposures, for example, T. aquaticus that is used in PCR. Psychrophiles are those that require low temperatures of less than 20⁰C while mesophiles require 20-40⁰C. Thermophiles, on the other hand, require high temperatures of above 40⁰C to survive. Most bacteria that infect human are mesophiles and grow optimally at 35-37⁰C. Bacteria that have endospores are more likely to survive extreme environmental conditions such as high temperature and high UV irradiation (Prescott, John and Donald, 54)
Three TSA plates were acquired and labeled with a name, lab time, and temperature. Each was divided into three sections. Each section was marked with the abbreviation of the test organisms. A single streak (about 1cm) of each organism was made on its respective section. They were incubated at temperatures of 35⁰C incubator, Room temperature (or 25⁰C incubator) and Refrigerator (~10⁰C).
Four TSA plates were acquired for each bacterium. Each was labeled with the name, lab time and the organism name. The plates were then divided into half each and labeled control and UV on each side. A sterile swab was inoculated into the culture. Three zigzag lines were made on the agar surface at 45 degrees to each other. The plates were exposed to UltraViolet light for 3 minutes before incubation at 35⁰C for 48 hours.
Four Trypticase Soy Broth tubes were labeled with name, lab time, organism and time. Each tube was inoculated with the appropriate organism. Each student in the team exposed the organism to different temperature of 40⁰C, 55⁰C, 80⁰C and ~100⁰C. Each organism was exposed to heat for a given length of time, that is, 10, 20, 30 and 40 minutes. The tubes were vortexed after every 10 minutes. After the appropriate length of time, the tubes were dried and incubated at 35°C for 48 hours.
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