Nobody downloaded yet

Solvent and Polarity Effects in Thin Layer Chromatography - Lab Report Example

Comments (0) Cite this document
In TLC, the mobile phase comprises of solvents such as ethanol, ethyl acetate and aceton while the stationary phase comprises of a solid that has absorption properties such as…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER97.9% of users find it useful
Solvent and Polarity Effects in Thin Layer Chromatography
Read TextPreview

Extract of sample
"Solvent and Polarity Effects in Thin Layer Chromatography"

Download file to see previous pages The adsorption chromatography is the most common type of TLC that analysts use in the labs to carry out their experiments from the many types of TLCs that exist (Snyder 57). Solvents with low volatility and with low viscosities are mostly used in the TLC experiment. Some of the commonly used solvents in the TLC process include hexane, ethanol, pentane, hydrocarbon mixture, acetone, ethyl acetate and diethyl ether because they are much cheaper and due to the fact that they are non-toxic. The distance that a compound travels on the silicon plate will depend largely on the compound’s affinity for the absorption material (Silicon plate) in comparison with the eluent (the solvent in use). This experiment determines the effect of polarity on elution as well as determines the TLC of an unknown organic compound.
120 mL jars, filter papers, hexane, ethyl acetate, acetone, ethanol, measuring cylinder, silica gel, TLC plates, lead pencils, trans-stilbene, 9-flourenone, benzoic acid, methylyene chloride, spotter and Iodine.
5. In a separate set up, dissolve in a test tube 2 mg of benzoic acid, 9-fluorenone and trans-stilbene in 10 drops of methylene chloride. Label each test tube. Record the observations in each test tube.
6. Use a spotter to apply a little amount of trans-stilbene at the first point on the first line on one plate very briefly using a spotter. Here, the solution should move by capillary action. Similarly, apply some amount of benzoic acid and 9-fluorenone on the second and the third line respectively. Look at the spots under ultraviolet light to see clearly. NB: It is important to protect one’s eyes using a protective device like a goggle to avoid eye contamination from the dangerous ultraviolet rays. Add some little solvent in each line of the plate again if the lines are not clearly visible. Record ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Solvent and Polarity Effects in Thin Layer Chromatography Lab Report”, n.d.)
Retrieved from
(Solvent and Polarity Effects in Thin Layer Chromatography Lab Report)
“Solvent and Polarity Effects in Thin Layer Chromatography Lab Report”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Distillation- Gas Chromatography Investigations
This energy is utilized by the molecules to change phase, that is, from the liquid phase into the gas phase. The temperature at which the liquid changes into vapor, in which case, the vapor pressure of a liquid at this temperature equals to the atmospheric pressure, and it is referred to as the boiling point.
4 Pages(1000 words)Lab Report
Introduction Thin-layer chromatography is a widely used analytical technique where the components of a sample are separated over a sorbent layer by the movement of an eluent by capillary action. The differences in migration and the attraction of the components to the eluent cause the separation on the TLC plate (Wall 2005).
5 Pages(1250 words)Lab Report
Pyrolysis of Aryl Sulfonate Esters in the Absence of Solvent: E1 or E2
e the aryl sulfonyl ester obtained and further determine whether the reaction mechanism for the pyrolysis of the aryl sulfonate ester was either E1 or E2 depending on the gas chromatographic evaluation of the pyrolysis products. Synthesis of methyl N-acetylsulfanilate, an aryl
2 Pages(500 words)Lab Report
Estimiting the half - value layer thikness
Shielding as control method is mainly utilized in reducing the external radiation hazard. The suitable material to use depends on the
2 Pages(500 words)Lab Report
Size Exclusion Chromatography- Protein Separation Polyacrylamide Gel Electrophoresis
To determine the molecular weight of the unknown, a standard solution containing a mixture of polymers of known molecular weight was used to calibrate the column. Table 1 below
1 Pages(250 words)Lab Report
(GC Instrument Experiment) Determination of Ethanol Concentration in Biological Fluids using Gas Chromatography
It was established that the ethanol concentration of the unknown was 13.805 ± 1.31% w/v. High concentrations of ethanol (ranging between 0.3 and 0.4%) in the blood often lead to fatal consequences that include death.
3 Pages(750 words)Lab Report
Distillation and Gas Chromatography: Separation of Hexanes and Toluene
The methods utilized in the experiment involved precise raising and falling of temperatures at specific temperature ranges to obtain three
4 Pages(1000 words)Lab Report
e polarity of the solutions relative to the stationary phase caused the observed differences among the chromatograms obtained using the different solvents. On the other hand, the relative polarity of the dyes resulted to differences in their interactions with the phases, and
6 Pages(1500 words)Lab Report
Thin-Layer Chromatographic Analysis
1) In some cases aspirin has the ability to lower the risk of a possible heart attack. Any patient is not advised to start taking aspirin without doctor’s consent. But it is highly likely that aspirin will be recommended to patients who had a
4 Pages(1000 words)Lab Report
The effects of salt solution on frog skin cell
The frog skin is composed of two layers (epidermis and dermis), is permeable to water and respiratory gases ( O2 and CO2 ) and ions such as Na+ and Cl-. The outermost layer
3 Pages(750 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Solvent and Polarity Effects in Thin Layer Chromatography for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us