Microbiology and Chemistry, Bacterial and Fungal Infections - Assignment Example

Insert your image as a jpeg in the box below, do not copy and paste the image as it may not resize correctly. 4. Using the data in the table above, plot a calibration curve of absorbance vs concentration and insert an image of the graph here. Hint – remember that you calibrated the spectrophotometer with water prior to taking these readings. Insert your image as a jpeg in the box below, do not copy and paste the image as it may not resize correctly. (2 marks) 6. In session 1, you were given a sample of amoxicillin sodium of unknown concentration.

Using the equation generated from the above calibration curve, what was the concentration of this sample? (1 mark) It involves correcting and estimating baseline effect through different algorithms. Base line variation occurs in many spectral data. It is nonlinear or linear addition to the spectra that results to expected zero which attains a value that is positive. Baseline is a varying curve that is slowly going through the lower spectra part without the peak jumps. 9. The instructions on the paediatric suspension bottle indicate that the final volume should be 100 mL but indicates only 84 mL potable water needs to be added.

Explain briefly why only 84 mL water is required to reconstitute this suspension? (1 mark). 10. In Session 2 and 3, you assessed the antimicrobial activity of amoxicillin sodium and amoxicillin trihydrate against two bacteria, E. coli and S. epidermidis. Using the data generated from this experiment, plot a calibration curve for each formulation on a single graph (against both bacteria); insert your calibration curve below. Insert your image as a jpeg in the box below, do not copy and paste the image as it may not resize correctly. (3 marks) According to curve, it can be concluded that Amoxicillin Trihydrate has a higher antibacterial effect than the Amoxicillin Sodium on both E-coli and S.

epidermidis. This is because both gradient line of Amoxicillin Trihydrate is steeper than that