Nobody downloaded yet

Enzymology - Lab Report Example

Comments (0) Cite this document
This paper investigates the factors affecting the catalase enzyme activity to the conclusion that temperature, pH, substrate concentration, and presence of enzyme inhibitors influence activity of the…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER91.9% of users find it useful
Read TextPreview

Extract of sample "Enzymology"

Download file to see previous pages Substrate concentration is directly proportional to activity rate up to a point beyond which the reaction rate slows down and settles at a constant level because of the enzyme’s limiting factor (Yada, 2004; Whitehurst & Oort, 2009). Temperature and pH, however, increase the activity rate up to optimum levels beyond which the activity rate reduces. The optimum temperature and pH level for most enzymatic activity is room temperature and the pH level of seven (Rastogi, 2010). Enzyme inhibitors are another set of factors that reduce enzymes’ activity rate (Seager & Slabaugh, 2010).
This report investigates the effects of substrate concentration, pH level, temperature and inhibitor hydrogen peroxide on bovine liver’s activity rate. It seeks to answer the question ‘how does the factors affect activity rate of Bovine liver?’
Care was taken to ensure that the oxygen gas sensor was plugged into the USB interface to launch the PAS portal window. This was followed by a click on the DataStudio icon to launch the program. The ‘create experiment’ button was then clicked to generate a ‘Digit’ box. A click on the setup button and a change of oxygen’s measurement unit to ppm then followed before calibration of the sensor. Amounts of liver extract were then incubated at given temperatures for five minutes before addition of substrate and push on the oxygen sensor stopper. With the stopper firmly held, the substrate was mixed and incubated while noting the amount of released oxygen. The experiment was repeated for different temperatures.
With the set ups at the room temperature, tests were conducted at the pH levels of two, four, six, seven, eight, ten, and twelve respectively. In each set up and for every pH level, 6 ml of buffer, 3 ml of liver extract and 4 percent of H2O2 were mixed in that consecutive order. This was followed by measurement of the level of generated oxygen for calculating reaction rates at each pH level.
With the ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Enzymology Lab Report Example | Topics and Well Written Essays - 1250 words”, n.d.)
Retrieved de
(Enzymology Lab Report Example | Topics and Well Written Essays - 1250 Words)
“Enzymology Lab Report Example | Topics and Well Written Essays - 1250 Words”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document


Biology Research Paper on Galactosemia

.... The disease can be treated by avoiding milk, as well as milk products. Prevention can be done through genetic counseling, especially in a family where they have had the history of the disease. References Bosch, A.M., Martha, A. G. Henk, D. B., Hugo, S. A. H., Fritz, A.W. & Bob, F. L. (2004). Living with Classical Galactosemia: Health-Related Quality of Life Consequences. Pediatrics, Vol. 113 Issue 5, 423-428. Bosch, A. M. (2006). Classical galactosemia revisited. Journal of Inherited Metabolic Disease, 29 (4), 516-525. Doyle, C. M., Channon,S. & Orlowska, P. J. (2010). The neuropsychological profile of galactosaemia. Journal of inherited metabolic disease, 33 (5), p603-p609 Flynn, A. (2011). Galactosemia Chapter. Encyclopedia of Dairy...
4 Pages(1000 words)Research Paper

Determination of Proteins Using Biuret and Lowry Assay Techniques

...., Bardawill, C. J. & David, M. M. (1949). Determination of Serum Proteins by means of the Biuret Reaction, Journal of Biological Chemistry, 5(3), pp. 177- 751. Kołodziejska, I., Sikorski, Z.E & Niecikowska, C. (1999). Parameters affecting the isolation of collagen from squid (Illex argentinus) skins, Food Chemistry, 66, pp. 153- 157. Layne, E. (1957). Spectrophotometric and Turbidimetric Methods for Measuring Protein Methods in Enzymology, Bio- Chemistry Journal, 3, p. 447. Lin, Y.K. & Liu, D.C, (2006). Effects of pepsin digestion at different temperatures and times on properties of telopeptide-poor collagen from bird feet, Food Chemistry, 94, pp. 621-625. Liu, H., Li, D. & Guo, S. (2007). Studies on collagen from...
5 Pages(1250 words)Essay

The Effects of Temp & PH on Enzymes

...units at 370C and it decreased to 0.06 units at 650C and further decreased to 0.05 at 1000C. Similarly, from table 2 on Class data of -amylase activity at different temperatures the activity of the was 0 at 40C, increased to 0.135 units at 230C and reached its peak of 0.34 units at 370C and decreased to 0.01 at 650C and further decreased to 0.045 units at 1000C. From the above results it is evident that the activity of -amylase is at its highest at body temperature of 370C, a confirmation that enzymes work optimally at this temperature. This is also shown by graph 1 above. Table 3 indicates that at lower pH values, enzyme reactions are lower rising towards neutral Ph and peaks at 7. This fact is illustrated by graph 2 above....
6 Pages(1500 words)Lab Report

Folding and Aggregation

... refolding Versus aggregation: computer simulations on an intermediate-resolution protein model Journal of Molecular Biology,Volume 312, Issue 1,7 September Pages,187-202 Urbanc, Brigita Borreguero, Jose Cruz M. Luis and Stanley H. Eugene (2006) Ab initio Discrete Molecular Dynamics Approach to Protein Folding and Aggregation Methods in Enzymology,Volume 412, Pages 314-338 Images from Figure I - accessed 2007 Figure II - accessed 2007 on a Knife Edge: Tipping the Balance between Folding and Aggregation This discussion is focused on the nature, structure and functions of proteins, how...
4 Pages(1000 words)Essay


...increasing remarkably....(microbiology) Cell and Molecular biology This pertains to the study of cells and the method of interaction of cellular components that are responsible to promote life processes. While the study of this topic would include the synthesizing of DNA and RNA, it also helps in the research to find the mechanism for differentiation of a single-celled egg into a complex multi-cellular organism. For studying the concepts of biotechnology or genetic engineering, the detailed knowledge of cell and molecular biology is very important. The biologists concerned with this relevant topic use its results and findings to study the principals of protein synthesis, enzymology, organelle structure and function and...
2 Pages(500 words)Article

Determination of Protein using Biuret Reagent

...increase in absorbance with an increase in protein concentration (Layne, 1957, 23). Analysis The reagent is made up of potassium hydroxide. It also has traces of hydrated copper (II) sulphate and potassium sodium titrate. The blue color appears as a result of presence of proteins, pink and blue may also arise in the presence of short chained polypeptides. A biuret reagent is not required in all protein tests. It is commonly used in biuret protein assay. On the other hand, a colorimetric assay is used in the determination of protein concentration. . I deal examples entail UV-VIS wavelength of 540 nm in the detection of Cu2+ ion (Layne, 1957, 23). Conclusion Biuret reagent is one of the common reagents used protein tests. It is highly...
3 Pages(750 words)Lab Report

Unit 7 paper biochem

...). A glucogenic amino acid is an amino acid that gives rise to glucose oxidation or intermediates of the TCA cycle. The end product of gluconeogenesis is glucose. These pathways are of utmost importance during working out because they ensure that the body is well supplied with energy during exercises. References Keeton, W. T., & Gould, J. L. (1993). Biological science (5th ed.). New York: Norton & Company. Nelson, D. L., & Cox M. M. (2004) Lehninger principles of biochemistry (4th ed.). New York, NY: W. H. Freeman & Company. Smith, J. (n.d.). Energy usage during exercise: How it affects your workouts. Retrieved from Wanders, J .A. R., Ruiter, J....
3 Pages(750 words)Essay

Describe the function of tolc gene in salmonella and how this function may relate to intracellular pathogenisis in this organism

..., and other germs, and also chemical composites. It is however important to elucidate the fact that in salmonella, TolC relies on other mechanisms for accountable for the removal of noxious substances from the cells. According to Horiyama, Yamagichi and Nishino (2010), these other mechanisms include "AcrAB, AcrD, AcrEF, MdtABC, MdsAB, EmrAB, and MdfA, MacAB, and MdtK" (p. 1372). In a nutshell, the function of the TolC gene in salmonella is aiding in the secretion of noxious substances from the cells of salmonella. References Horiyama, T., Yamaguchi, A. & Nishino, K. (2010). TolC dependency of multidrug efflux systems in Salmonella enterica serovar Typhimurium. Journal of Antimicrobial Chemotherapy, 65, 1372-1376. ...
1 Pages(250 words)Essay

Protein isolation

...Protein Purification Protein Purification Introduction Majority of scientific studies today require the isolation, purification and study of micro-molecules in biochemical processes. The existence of a myriad of protein isolation and purification techniques is a direct consequence of these scientific studies (Jordan and Ogren, 1981). Enzymes are among the most studied proteins due to their crucial role in regulating metabolic processes. Enzymology entails the use of isolation and purification techniques similar to those used in proteomics. Ribulose 1, 5-bisphosphate carboxylase/oxygenase (rubsico) is one of the most abundant proteins in the universe a reaction on the synthesis of sugar in green plants (Jordan and Ogren,...
2 Pages(500 words)Lab Report


... Importance of enzymes as catalysts Enzymes play a significant part in altering the reaction rate in all biological reactions, where they are present. Mostly, enzymes are in form of proteins in nature. By altering the reaction rate, it means that they either slow or make fast the rate of reaction. Proteins fundamentally act as enzymes which enhance the rate of reaction inside the cells. Most biological reactions are altered or catalyzed by enzymes (proteins). Without enzymatic catalysis, reactions that are biological would not take place because they are so slow in nature, especially under temperature conditions that are mild and pressure that makes them compatible with life. Enzymes do accelerate reaction rate by more than 1... Importance...
4 Pages(1000 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic Enzymology for FREE!

Contact Us