quora
Nobody downloaded yet

Enzymology - Lab Report Example

Comments (0) Cite this document
Summary
This paper investigates the factors affecting the catalase enzyme activity to the conclusion that temperature, pH, substrate concentration, and presence of enzyme inhibitors influence activity of the…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER96.8% of users find it useful
Enzymology
Read TextPreview

Extract of sample
"Enzymology"

Download file to see previous pages Substrate concentration is directly proportional to activity rate up to a point beyond which the reaction rate slows down and settles at a constant level because of the enzyme’s limiting factor (Yada, 2004; Whitehurst & Oort, 2009). Temperature and pH, however, increase the activity rate up to optimum levels beyond which the activity rate reduces. The optimum temperature and pH level for most enzymatic activity is room temperature and the pH level of seven (Rastogi, 2010). Enzyme inhibitors are another set of factors that reduce enzymes’ activity rate (Seager & Slabaugh, 2010).
This report investigates the effects of substrate concentration, pH level, temperature and inhibitor hydrogen peroxide on bovine liver’s activity rate. It seeks to answer the question ‘how does the factors affect activity rate of Bovine liver?’
Care was taken to ensure that the oxygen gas sensor was plugged into the USB interface to launch the PAS portal window. This was followed by a click on the DataStudio icon to launch the program. The ‘create experiment’ button was then clicked to generate a ‘Digit’ box. A click on the setup button and a change of oxygen’s measurement unit to ppm then followed before calibration of the sensor. Amounts of liver extract were then incubated at given temperatures for five minutes before addition of substrate and push on the oxygen sensor stopper. With the stopper firmly held, the substrate was mixed and incubated while noting the amount of released oxygen. The experiment was repeated for different temperatures.
With the set ups at the room temperature, tests were conducted at the pH levels of two, four, six, seven, eight, ten, and twelve respectively. In each set up and for every pH level, 6 ml of buffer, 3 ml of liver extract and 4 percent of H2O2 were mixed in that consecutive order. This was followed by measurement of the level of generated oxygen for calculating reaction rates at each pH level.
With the ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Enzymology Lab Report Example | Topics and Well Written Essays - 1250 words”, n.d.)
Retrieved de https://studentshare.org/biology/1603904-enzymology
(Enzymology Lab Report Example | Topics and Well Written Essays - 1250 Words)
https://studentshare.org/biology/1603904-enzymology.
“Enzymology Lab Report Example | Topics and Well Written Essays - 1250 Words”, n.d. https://studentshare.org/biology/1603904-enzymology.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
The Effects of Temp & PH on Enzymes
This is because they have unique active sites into which only specific substrates can fit. Enzymes bind temporarily to one or more of the reactants of the reaction they catalyze and in doing so; they lower the amount of activation energy needed and thus speed up the reaction (Groves, 1997).
6 Pages(1500 words)Lab Report
Enzyme Amylase Lab Report
It proved that high enzyme concentrations and optimum pH levels and temperatures increased enzyme activity. Introduction: Amylase is an enzyme that helps in breaking down starch to form sugars such as maltose and glucose. In human beings it is found in the saliva.
5 Pages(1250 words)Lab Report
Biology
These include the application of microbiological knowledge for the industrial fermentation that produces various food beverages, apart from
2 Pages(500 words)Article
Determination of Protein using Biuret Reagent
In case the bond is detected, copper (II) ions turn violet in an alkaline solution. In performing this test, several variants can be considered. Despite the name, the chemical does not contain burette ((H2N-CO-)2NH). This reaction is named using that name because it
3 Pages(750 words)Lab Report
Proteins
One such true and accurate method of protein concentration determination involves using the acid hydrolyzing of a portion of sample and carrying out amino acid
5 Pages(1250 words)Lab Report
Enzymology
Most biological reactions are altered or catalyzed by enzymes (proteins). Without enzymatic catalysis, reactions that are biological would not take place because they are so slow in nature, especially
4 Pages(1000 words)Lab Report
Describe the function of tolc gene in salmonella and how this function may relate to intracellular pathogenisis in this organism
ing to Horiyama, Yamagichi and Nishino (2010), "Salmonella enterica is a pathogen that causes a variety of diseases in humans, ranging from gastroenteritis to bacteraemia and typhoid fever" (p. 1372). TolC is considered a superficial casing conduit. This gene is involved in the
1 Pages(250 words)Essay
Unit 7 paper biochem
The phosphagen system depends on stored ATP to provide immediate energy. The glycolytic energy system (anaerobic) involves partial breakdown of glucose into pyruvate to give small amounts of energy. This pathway is significant in
3 Pages(750 words)Essay
Protein isolation
Enzymology entails the use of isolation and purification techniques similar to those used in proteomics. Ribulose 1, 5-bisphosphate carboxylase/oxygenase (rubsico) is one of
2 Pages(500 words)Lab Report
Mass Spectrometry and HPLC of peptides
Soft ionization converts the macromolecule under investigation in the presence of a polar volatile solvent into tiny droplets, which are further desolvated into smaller droplets hence forming molecules that are
3 Pages(750 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Enzymology for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us