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Enzymology - Lab Report Example

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This paper investigates the factors affecting the catalase enzyme activity to the conclusion that temperature, pH, substrate concentration, and presence of enzyme inhibitors influence activity of the…
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Download file to see previous pages Substrate concentration is directly proportional to activity rate up to a point beyond which the reaction rate slows down and settles at a constant level because of the enzyme’s limiting factor (Yada, 2004; Whitehurst & Oort, 2009). Temperature and pH, however, increase the activity rate up to optimum levels beyond which the activity rate reduces. The optimum temperature and pH level for most enzymatic activity is room temperature and the pH level of seven (Rastogi, 2010). Enzyme inhibitors are another set of factors that reduce enzymes’ activity rate (Seager & Slabaugh, 2010).
This report investigates the effects of substrate concentration, pH level, temperature and inhibitor hydrogen peroxide on bovine liver’s activity rate. It seeks to answer the question ‘how does the factors affect activity rate of Bovine liver?’
Care was taken to ensure that the oxygen gas sensor was plugged into the USB interface to launch the PAS portal window. This was followed by a click on the DataStudio icon to launch the program. The ‘create experiment’ button was then clicked to generate a ‘Digit’ box. A click on the setup button and a change of oxygen’s measurement unit to ppm then followed before calibration of the sensor. Amounts of liver extract were then incubated at given temperatures for five minutes before addition of substrate and push on the oxygen sensor stopper. With the stopper firmly held, the substrate was mixed and incubated while noting the amount of released oxygen. The experiment was repeated for different temperatures.
With the set ups at the room temperature, tests were conducted at the pH levels of two, four, six, seven, eight, ten, and twelve respectively. In each set up and for every pH level, 6 ml of buffer, 3 ml of liver extract and 4 percent of H2O2 were mixed in that consecutive order. This was followed by measurement of the level of generated oxygen for calculating reaction rates at each pH level.
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