Download file to see previous pages
As stated by Onteh et al (2005), most biochemical reactions would be very slow without enzymes since enzymes increase the rate of biochemical reactions by thousands times. It is therefore important to study the environmental factors: pH and temperature and how they would affect specific enzymes.
There are three characteristics of enzymes that make them function as catalysts. First enzymes increase the rate of biochemical reaction, second, they are specific to a substrate and thirdly they can be regulated to increase or reduce rate of biochemical reaction. Studies have confirmed that enzymatic activities are affected by certain factors such as enzyme concentration, pH, and temperature as well as substrate concentration. According to (), all these factors except substrate concentration have the effect of modifying the specificity nature of the enzyme there by making it incompatible with the substrate. Moreover enzymes act as catalysts because they exhibit three dimensional protein structures. As stated by Onteh et al (2005), this structure is particularly sensitive to changes in salts, pH and temperature thus a slight changes in temperatures of reaction can significantly change the rte of reaction while extreme temperatures can irreversibly alter the both the three dimensional structure of the enzyme thus making it impossible to catalyze a reaction.
The enzymatic activity is based on the fact that enzymes are made up of certain active sites that are specific to the substrate’s active sites. As stated by Onteh et al (2005), it is on this active sites that enzymes bind to a given substrate in a geometrical fashion after which substrate undergo its inherent reaction at a much faster rate. This mode of enzymatic action is referred to as lock and key. It is important to note that enzyme does not actually react with a substrate, but brings and aligns the substrate in order for it to react with other substances. Enzymes therefore have
...Download file to see next pagesRead More
(“Enzymes Lab Report Example | Topics and Well Written Essays - 2000 words”, n.d.)
Enzymes Lab Report Example | Topics and Well Written Essays - 2000 words. Retrieved from https://studentshare.org/biology/1659566-enzymes
(Enzymes Lab Report Example | Topics and Well Written Essays - 2000 Words)
Enzymes Lab Report Example | Topics and Well Written Essays - 2000 Words. https://studentshare.org/biology/1659566-enzymes.
“Enzymes Lab Report Example | Topics and Well Written Essays - 2000 Words”, n.d. https://studentshare.org/biology/1659566-enzymes.
This is because they have unique active sites into which only specific substrates can fit. Enzymes bind temporarily to one or more of the reactants of the reaction they catalyze and in doing so; they lower the amount of activation energy needed and thus speed up the reaction (Groves, 1997).
Inhibitors also affect working of enzyme by competitively, non-competitively and mixed reaction.
Method: a calibration graph that had been previously prepared was used. A Line-weaver Burk was plotted with reciprocal of substrate plotted against reciprocal of
Method: Three samples of DNA are extracted from bacteriophageand purified. These Purified samples of DNA number 48,502 base pairs in length. These samples are now segregated into four and are incubated at 37 combined individually with three different restriction endonucleases and the fourth sample, which is used, as a negative control does not have any endonuclease introduced into it.
Many enzymes work well in a narrow range of pH. A change in pH may alter the three dimensional structure of the enzyme due to formation or breakage of covalent bonds within the enzyme. A change in the structure
According to the research, enzymes are the chemical molecules made of proteins and catalyze various biological reactions for the survival of all living organisms on the planet. They are highly specific to the conditions in their vicinity. Any alteration in the conditions, make them inactive as modified conditions alter the shape of the protein molecules.
The results show that lactase activity is low when temperature is low at 00C but improves with increasing temperature up to 370C after which activity drops sharply. Low activity at low temperature is due to low activation energy exhibited by
The extent of hydrolysis was based on the time of reaction. The product is glucose if starch was completely hydrolyzed. The absence or presence of starch in the solutions was tested using iodine. Iodine never reacts with glucose, instead, forms a blue to black complex with starch.
5 Pages(1250 words)Lab Report
GOT A TRICKY QUESTION? RECEIVE AN ANSWER FROM STUDENTS LIKE YOU!
Save Your Time for More Important Things
Let us write or edit the lab report on your topic
with a personal 20% discount.