The present report is focused on the peculiarities of the lactase enzyme activity. The first thing to be mentioned is that enzymes are biological molecules made up of a complex protein that catalyzes specific chemical change in living organisms. …
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Microfuge tubes were selected and labeled according to the selected temperatures 00C, 250C, 400C, 600C, 800C and 1000C and each filled up with 0.5 lines with lactase solution. The solutions were then maintained at water baths with respective temperatures for five minutes after which milk was added to lactose solution up to 1.0 line using an alternate plastic pipette. The solution was left for ten minutes after which a glucose strip was placed into each of the test tubes for one second and allowed to sit on the bench for thirty seconds. The coloration of the glucose strip was then compared with the chart and the amount of glucose recorded in mg/dL.
Seven microfuges were selected and labeled; 2, 4, 5, 6, 7, 8, 10, and 12, filled with appropriate pH buffer up to 0.5 line and 3 drops of milk added into each tube. The solution solutions in the seven tubes were then mixed by inverting the tubes three times after which 3 drops of lactase solution was added to each tube using a plastic pipette. All tubes were then incubated for 10 minutes in a water bath at 400C. A glucose strip was then placed in each test tube for one second, removed and left on the bench for thirty seconds and finally coloration compared to the chart provided.
Two microfuge tubes were selected and labeled “L” and the other labeled “M”. Lactose and Maltose solutions were then added into the tubes labeled L and M respectively up to the 0.5 line.
Lactase solution was then added into each tube up to 1.0 line and the mixture placed in 400C water bath for 10 minutes.
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The researcher utilized Lactaid which is an over the counter tablet as a source of enzymes, known to particularly digest protein and pectin. Obviously, the research adopted the experimental method. So, a control and an experimental set-up were installed for observations.
It proved that high enzyme concentrations and optimum pH levels and temperatures increased enzyme activity. Introduction: Amylase is an enzyme that helps in breaking down starch to form sugars such as maltose and glucose. In human beings it is found in the saliva.
In general terms this was to introduce concepts related to enzyme kinetics. Introduction Enzymes are among several proteins generated in living cells to catalyze or accelerate the organism’s metabolic processes. Normally, enzymes are so selective and specific on the molecules they catalyze, referred to as substrate (Dixon, 1979).They normally do react specifically with a single substrate.
The enzymes have specialized structures that work according to lock and key model. An enzyme is specific and cannot activate all kind of reactions, as a key can open certain lock only.
Enzymes play a vital role
The study of the activities of enzymes that help in existence of pathogens can help in developing the drugs, which decrease that activity and thus play an active role in preventing the pathogenical activities.
The enzyme activities that play a major role in conversion of muscle from one form to other can be used in developing methods to restore muscle activity as the age increases in human beings and even in animals.
hrough the method of extracting the enzyme by blending it with potato tissue in phosphate buffer, the effects were analyzed on the effect of the dye guaiacol and the activity measured under different temperatures. The optimum temperature was obtained at 22.20C and above this
The results show that lactase activity is low when temperature is low at 00C but improves with increasing temperature up to 370C after which activity drops sharply. Low activity at low temperature is due to low activation energy exhibited by
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