Nobody downloaded yet

B-Galactosidase Enzyme Activities - Essay Example

Comments (0) Cite this document
Summary
The essay "B-Galactosidase Enzyme Activities" delivers a summary of the structure, function, and catalytic mechanism of lacZ β-galactosidase. The main aim of the experiment was achieved since quantification of B-galactosidase enzyme found in the E.coli was successful…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER95.8% of users find it useful
B-Galactosidase Enzyme Activities
Read TextPreview

Extract of sample
"B-Galactosidase Enzyme Activities"

Download file to see previous pages The ideas on the regulation of protein synthesis in bacteria have mainly arisen from the studies of Perse Monod on the induced synthesis of B-galactosidase in E.coli. It resulted in the discovery of regulatory events to the transcription of DNA and the assemblage of functionally related genes into clusters called operons. The specific mechanisms exist to regulate the levels of expression in the cell. The regulation may be as a result of transcription, translation or messenger RNA stability. In this experiment, there is the regulation of transcription of bacterial genes that are either inducible or repressible enzyme systems. In induced enzymes, there is the breakdown of complex molecules for example lactose induces synthesis of the proteins B-galactosidase, galactose permease and thiogalactoside transacetylase in E.coli. The b-galactosidase enzyme found in E.coli is an example of an inducible enzyme. It catalyzes the hydrolysis of a wide variety of B-galactosides such as lactose into its constituent substances
There was of sharing of the reagents labeled control and lactose (L) flask. It was vital that the lactose does not enter the control bottle. Separate, a labeled pipette was reserved to make an addition to, or to sample from the flask. Time was an important factor and lactose was timed at zero time, ninety minutes, hundred and twenty and one hundred and eighty minutes up to two hundred and forty minutes. From there, sampling procedure included. At the appropriate time, 3ml aliquots were removed from both control(C), and lactose (L) culture and further 0.5mL aliquots from lactose (L) culture placed in a plastic centrifuge tube. Dilution of 0.5mL aliquot one in five by adding 2ml of culture medium was done and labeled the cell L/5. (Michael & Nelson. 2008) ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“B-Galactosidase Enzyme Activities Essay Example | Topics and Well Written Essays - 1500 words”, n.d.)
Retrieved from https://studentshare.org/chemistry/1685529-practical-report-1-unit-name-introductory-molecular-biology-and-biochemistry-i
(B-Galactosidase Enzyme Activities Essay Example | Topics and Well Written Essays - 1500 Words)
https://studentshare.org/chemistry/1685529-practical-report-1-unit-name-introductory-molecular-biology-and-biochemistry-i.
“B-Galactosidase Enzyme Activities Essay Example | Topics and Well Written Essays - 1500 Words”, n.d. https://studentshare.org/chemistry/1685529-practical-report-1-unit-name-introductory-molecular-biology-and-biochemistry-i.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Enzyme Assays, Enzyme Kinetics
...?To Investigate and Measure Km and Vmax of ?-galactosidase Enzyme The main purpose of carrying out this experiment was to investigate and measure two parameters, Km and Vmax for ?-galactosidase enzyme. Furthermore the PH change effect on the enzyme activity was investigated as well. In general terms this was to introduce concepts related to enzyme kinetics. Introduction Enzymes are among several proteins generated in living cells to catalyze or accelerate the organism’s metabolic processes. Normally, enzymes are so selective and specific on the molecules they catalyze, referred to as...
6 Pages(1500 words)Lab Report
De-oxy-ribonuclease enzyme
...in different organisms because of its significance as a restriction enzyme. Literature Cited BUXBAUM, E. 2007.Fundamentals of protein structure and function.Springer, New York, USA. CHEN, W., & LIAO, T. 2006. Structure and function of bovine pancreatic deoxyribonuclease I. Protein And Peptide Letters, 13: 447-453. COCKERILL, P. N. 2011. Structure and function of active chromatin and DNase I hypersensitive sites. FEBS Journal, 278: 2182-2210. GUEROULT, M., PICOT, D., ABI-GHANEM, J., HARTMANN, B., &BAADEN, M., 2010.How Cations Can Assist DNase I in DNA Binding and Hydrolysis. Plos Computational Biology, 6: 1-11. PARSIEGLA, G., NOGUERE, C., SANTELL, L., LAZARUS, R., & BOURNE, Y. 2012. The...
3 Pages(750 words)Research Paper
Enzyme Kinetics
...-up were installed for observations. The results were tabulated, statistically treated and interpreted to reveal that enzymatic activities are affected by changes in temperature, such that as the temperature of the environment where the chemical reaction takes place increase, the chances of the rate of molecular collision increases causing a consistent increase in the production of glucose. The same concept holds true to ph, substrate concentration, and enzyme concentration. Nevertheless, the rate of molecular reaction facilitated by enzymes ceases when enzymes as protein molecules are denatured because of extremes in these factors. But, a supplementary...
14 Pages(3500 words)Lab Report
Enzyme Activity
...in animals (as well as in human beings). These activities are studied to solve the problems regarding the muscle problems. After the structure (skeleton) of the human body the muscle is the thing, which contributes strength and movement for it. The enzymes, which affect the fibre and the muscle, play an important role in developing methods to increase or preserve the efficiency of the muscle fibre. There are 3 types of fibres according to P. D. Gollnick, R.B. Armstrong, C.W.Saubertiv, K. Piehl, and B. Stalin (1972). These fibres are interconvertibility depends on the training given. The training proved to increase red muscle at the expense of white muscle. This means that the...
4 Pages(1000 words)Essay
Enzyme Linked Assays
... of a green active substrate complex I, HRP + H2O2 <========> Complex I 2. Transition of complex I to red complex II, Complex I + AH <====> Complex II + A, where AH is the donor in the reduced form and A is the donor in the oxidized form. 3. Recovery of the enzyme by reduction of complex II, Complex II + AH <====> HRP + A + 2H2O 4. Formation of the product 2A -------- A2 The substrate specificity of HRP in forming complex I is very high; and the only reagents that can combine with HRP to form an active substrate complex are hydrogen peroxide (H2O2), methyl hydrogen peroxide (MeHOO), and ethyl hydrogen peroxide (EtHOO). The specificity of the enzyme-substrate... The...
5 Pages(1250 words)Essay
Alkaline phosphatase enzyme
...hydrolases that are used as catalysts in the following general reaction at high pH (>7.0): R-O-PO3H- + H2O R-OH + H2PO4- The alkaline phosphatase enzyme performs the vital functions at cellular level in a number of different organisms whether they are eukaryotic or prokaryotic. Even though the physiological function of the alkaline phosphatase enzyme is not clear it still plays a vital role in the phosphate metabolism. A gene known as ALPL gives instructions for making the alkaline phosphatase enzyme. The role played by alkaline phosphatase enzyme is very important. It helps in the growth and development of bones and teeth. It is...
5 Pages(1250 words)Essay
Enzyme kinetics
...Enzyme Kinetics al Affiliation) Kinetic Properties of Enzymes a) i) The rate of the initial reaction The reaction rate of an enzyme-catalyzed reaction varies with substrate concentration. The common form of the equation is given by: Vo = Vmax (S) KM + (S) Where: Vo = initial reaction velocity Vmax = maximal reaction velocity when all enzyme active sites are filled with substrate molecules (S) = Substrate concentration KM = Michaels constant = K2+k3 K1 Rate = change in product Change in time Initial rate = 12-0 = 1.33 µmol min-1 9-0 ii) Effect of doubling the substrate There is a hyperbolic relationship between the rate of reaction... Part C...
1 Pages(250 words)Assignment
Enzyme Activity
...Enzyme Activity Introduction Enzymes are biological catalysts that are used in hastening biochemical reactions. Without the presence of enzymes, most chemical reactions in the body such as respiration would take a very long time to come to proceed. In addition, a lot of heat would be generated and lead to the destruction of living cells. Enzymes increase the rates of these reactions by lowering the activation energy of the reactions. Activation energy can be described as the least amount of energy that is needed for reacting substances to go through a reaction. An enzymatic reaction involves three main components namely...
8 Pages(2000 words)Lab Report
Enzyme lab
...Determining the Properties of an Enzyme Enzyme peroxidase is essential in any cell metabolic reaction as it breaks down the harmful hydrogen peroxide to harmful products in the body. The report analyzed its effect on changes in temperatures by determining the optimum temperatures and the effects of its reversibility. Through the method of extracting the enzyme by blending it with potato tissue in phosphate buffer, the effects were analyzed on the effect of the dye guaiacol and the activity measured under different temperatures. The optimum temperature was obtained at 22.20C and above this temperature, the enzyme was denatured. Conclusively, increase in...
2 Pages(500 words)Lab Report
Digestive Enzyme Experiment
...In general, pH level could highly affect the activities of enzymes (Simpson, 2012, p. 200). The given graph strongly suggest that enzymes like pepsin can work best at pH 2.5 level whereas trypsin can work best at pH 6.5 level. Both pepsin and trypsin are needed in hydrolyzing or digesting protein (Gropper and Smith, 2013, p. 40; Khurana, 2008, p. 361). Assuming that the pH level would either move above or below the pH 2.5 level, pepsin will not be able to digest protein properly. The same applies to the case of trypsin. Assuming that the pH level would either move above or below the pH 6.5 level, trypsin will not be able to digest protein properly. 3.3 Transport of Digested Amino Acid,...
9 Pages(2000 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic B-Galactosidase Enzyme Activities for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us