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Enzyme Kinetics - Lab Report Example

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To test how temperature, ph, substrate concentration, and enzyme concentration affect hydrolysis of lactose was the interest of this research. This was done to learn the structure and function of an enzyme, to learn the relationship between substrates, enzymes, and products, to understand how different factors can affect the rate of an enzymatic reaction, to learn about lactose, lactase, and how to test for the presence of glucose with a Diastix, and to turn in a formal laboratory report…
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Enzyme Kinetics
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The results were tabulated, statistically treated and interpreted to reveal that enzymatic activities are affected by changes in temperature, such that as the temperature of the environment where the chemical reaction takes place increase, the chances of the rate of molecular collision increases causing a consistent increase in the production of glucose. The same concept holds true to ph, substrate concentration, and enzyme concentration. Nevertheless, the rate of molecular reaction facilitated by enzymes ceases when enzymes as protein molecules are denatured because of extremes in these factors.

But, a supplementary enzyme from an artificially concocted tablet like Lactaid when taken is capable of augmenting enzyme concentration in case the need arises. Plants, animals, and microorganisms body growth and development are products of kinetics in metabolic pathways (Mader, 2001). The kinetics or the speeding-up, or the slowing down of chemical reactions in these metabolic pathways is caused by enzymes (Miller and Levine 2003). Enzymes are commonly known as protein molecules that are preoccupied with control of chemical reactions in the metabolic pathway. . Since enzymes are included in the process of metabolism, it is said that it also is responsible in producing all the chemicals in living organisms.

Additionally, it is also considered as important factor for the fast process of metabolism. But in every process there are different factors that enzymes are conditioned. These determinants greatly contributed for the dynamic process of a living organism's metabolism. There were different laboratory studies conducted which determined that the speeding-up and slowing-down of metabolism are affected by its surroundings changes in temperature, intense shift in pH (high or low), which in turn affects the efficiency of enzymes.

Another one is the substrate concentration, wherein this indicates the reaction of enzymes in a given condition, and lastly, the enzyme concentration which indicates the amount of enzymes in the substrates in a chemical reaction. This laboratory study revealed the important role of enzymes to sustain life. It indicated the progress of ones' metabolism with the proper condition of enzymes. There are thousands of enzymes that are known and each of it has their particular work. In every living organism, the metabolism process if very different from one of each other.

This will determine the metabolic enzymes that should be evident in for example, digestion. There are thousands of enzymes that are evident and are important in every living organism. One of this is the enzyme lactase. Lactase enzyme is needed for proper digestion of lactose that is evident in dairy products. This typical enzyme is familiarly known to determine the cause and effect of improper digestion in living organisms. As observed, majority of human adults are victims

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