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The Effect of Increasing Concentration of Enzyme - Lab Report Example

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The paper 'The Effect of Increasing Concentration of Enzyme' presents Rennin which was chosen as the enzyme and milk as its substrate since rennin is responsible for the curdling of milk in infants. The experiments very well demonstrated the saturation kinetics of the increasing enzyme…
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The Effect of Increasing Concentration of Enzyme
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Download file to see previous pages Enzymes are highly specific in their actions and act in different compartments inside the cells and in the extracellular body fluids, e.g., blood-clotting factors and the digestive enzymes. Abnormal or absent enzymatic reactions are responsible for a number of diseases or pathological conditions.
The catalytic activity of enzymes is not only important for productive intermediary metabolism, recycling, and digestion but is also important for cellular activities such as signal transduction and regulation of cellular functions, e.g., kinases/phosphatases and acetylases/deacetylases; generation of locomotion force, e.g., myosin (heavy chain - a protein serine/threonine kinase; and light chain - calcium/calmodulin-dependent kinase) hydrolyzes ATP to generate force for muscle contraction; and as ion pumps, e.g., ATPases in the cell membrane are involved in active transport.
All enzymes work within a range of temperatures, specific to the medium or organism they work in. The velocity of a chemical or enzyme reaction is almost doubled for every 10o C increase in temperature because the increase in temperature enhances the kinetic energy of the substrates and thus increases the proportion of substrates reaching the transition state (Kennel and Rodwell 2006). The rise in enzymatic activity continues until it reaches a maximum, known as the ‘temperature optima’ (Top).
The optimum temperature (Top), at which the reaction rate is maximum (Fig.1), generally ranges from 40 to 60o C, but differs from one system to the other, i.e., it is different for humans, cold-blooded animals, plants and thermophilic organisms. For most human enzymes. ...Download file to see next pagesRead More
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