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It is composed of 296 amino acids, one hemin group and one neutral carbohydrate side chain attached through asparagine. The molecular weight of the polypeptide part is 31,060, and including hemin and…
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Download file to see previous pages How the temperature of the solution influences the enzyme activity. It is expected that the enzyme will be destroyed at high temperatures and will work slowly at low temperatures because the enzyme is found in a plant that lives approximately at 10C-30C.
The influence of the pH on enzyme activity. Because the plant tissue that the enzyme is found in is not extremely acidic or basic, it is expected that extremes of pH will have a negative effect on enzyme activity.
Previously prepared enzyme extract at volumes of 0.5, 1.0 and 2.0 ml was added to tubes containing 1.0 ml guaicol dye (or 0 ml for control), 2.0 ml H2O2 (or 0 ml for control), and buffer (pH 5) at volumes of 3-5 ml to produce a total volume of 8.0 ml. Spectrophotometer readings at 500 nm were performed at 20 second intervals immediately after mixing the tubes for two minutes and the results were recorded.
Mixtures of 4 ml buffer (pH 5) (or 0 ml for control), 2.0 ml H2O2 (or 0 ml for control), 1.0 ml of extract (or 0 ml for control) and 1.0 ml guaicol dye (or 0 ml for control) were combined at a total volume of 8 ml, incubated at 4C, 22C, 32C and 48C and 100C
All the solutions were pre-incubated at the appropriate temperatures for 15 minutes to allow them to equilibrate before mixing. Spectrophotometer readings at 500 nm were performed at 20 second intervals immediately after mixing the tubes for two minutes and the results were recorded.
To determine the effects of pH mixtures of 4 ml buffer at pH 3, 5, 7 or 9 (or 5 or 0 ml for control), 2.0 ml H2O2 (or 0 ml for control), 1.0 ml of extract (or 0 ml for control) and 1.0 ml guaicol dye (or 0 ml for control) were combined at a total volume of 8 ml. Spectrophotometer readings at 500 nm were performed at 20 second intervals immediately after mixing the tubes for two minutes and the results were recorded
To see how inhibitors effect enzyme activity mixtures of 4 ml buffer (pH 5) (or 0 ml ...Download file to see next pagesRead More
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