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Conclusively, increase in temperature increases the activity of the enzyme up to the maximum level, and further increase leads to the denaturing of the enzyme.
For any chemical reaction to proceed within a cell, activation energy is required to cause and complete this process. This is made possible by the use of enzymes. Enzymes essentially work within the cells and their ability determined as a result of their specificity brought about by the shapes from the amino acid sequences (Daniel and Danson 2740).
The enzymes have active sites on their surfaces to allow the binding of a substrate through the help of coenzymes to form enzyme-substrate complex. The chemical reaction thus converts the substrate to a new product then released and the catalytic cycle proceeds.
Many factors, for example, pH and temperature affects the way enzymes work by either increasing the rate or determining the type of product produced (). The report, therefore, analyses the effects of the enzyme peroxidase in metabolic reactions and determining its optimum temperature in the reactions.
The effect of temperature was then determined on the activity of enzyme peroxidase through monitoring its effects under different temperatures of 4, 32 and 600C on the dye guaiacol when it turned brown.
Temperature increased from 40 C to room temperature of 22.20 C resulting to the increase of the peroxidase activity. This explained that increase in temperature up to the optimum point led to the increase of the breakdown of guaiacol dye to color brown with intensity increasing up to this optimum point. Further increase in temperature to 60C denatured the enzyme and therefore the production of the brown color stopped and its intensity reduced.
From the experiment therefore, the optimum temperature for peroxidase is at 22.20C from graph on figure 1. The temperature effect is reversible in the case whereby the peroxidase is exposed to temperatures that negatively affect
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(Enzyme Lab Report Example | Topics and Well Written Essays - 500 Words)
Enzyme Lab Report Example | Topics and Well Written Essays - 500 Words. https://studentshare.org/biology/1684103-enzyme-lab.
“Enzyme Lab Report Example | Topics and Well Written Essays - 500 Words”, n.d. https://studentshare.org/biology/1684103-enzyme-lab.
The results show that lactase activity is low when temperature is low at 00C but improves with increasing temperature up to 370C after which activity drops sharply. Low activity at low temperature is due to low activation energy exhibited by
It is highly imperative to classify bacteria and to identify microorganisms as they have enough potential to cause various diseases. Essentially, identification process is highly pragmatic for the correct diagnosis and treatment. Identification of the bacteria provides line of treatment.
According to the author of the report, enzymes are capable of regulating the rate at which chemical reactions occur without being altered in the process. To be precise, enzymes achieve this function by lowering the activation energy necessary for the reaction to occur in cells.
It proved that high enzyme concentrations and optimum pH levels and temperatures increased enzyme activity. Introduction: Amylase is an enzyme that helps in breaking down starch to form sugars such as maltose and glucose. In human beings it is found in the saliva.
In general terms this was to introduce concepts related to enzyme kinetics. Introduction Enzymes are among several proteins generated in living cells to catalyze or accelerate the organism’s metabolic processes. Normally, enzymes are so selective and specific on the molecules they catalyze, referred to as substrate (Dixon, 1979).They normally do react specifically with a single substrate.
This difference was because of the differences in the concentration of yeast cells that produces catalase enzyme directly proportion to the amount in each tube. The higher the concentration of catalase enzyme the higher the rate of reaction and vice versa.
There are certain factors that influence the activity of catalase:
• Substrate Concentration
• Salt Concentration
• Presence of Inhibitors
• Presence of Activators
Hence even if the same experiment is performed several times different results may lead to different results.
This was also evident in our class. The frequency in our group was 71 percent and is close to what is expected in any group of people. This implies the gene for non-tasters is recessive gene, which maybe arises from a defect in an enzyme
3 Pages(750 words)Lab Report
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