Contact Us
Sign In / Sign Up for FREE
Go to advanced search...

Malate Dehydrogenase Isoenzymes - Lab Report Example

Comments (0) Cite this document
The lab report "Malate Dehydrogenase Isoenzymes" points out that This experiment examined the localization of three enzymes within liver cells. The enzymes being examined were malate dehydrogenase (MDH), lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH)…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER95.5% of users find it useful
Malate Dehydrogenase Isoenzymes
Read Text Preview

Extract of sample "Malate Dehydrogenase Isoenzymes"

Download file to see previous pages The activity of protein also varied between the fractions, with the liver particulate and lysed liver particulate showing higher levels of protein than the supernatant. The results of the experiment indicate that the majority of energy converting reactions is centered within the mitochondria, with the MDH reaction causing the highest amount of absorption, suggesting a stronger reaction. The prevalence of protein within this portion of the cell supports this hypothesis. The use of electron acceptors to measure the strength of reactions was an effective approach to determining the prevalence of each reaction, as well as their localization within the cell.

Throughout the human body, different enzymes interact with one another to form comprehensive reactions and chains of reactions that play important roles in the functioning of the human body (Schilling et al., 1999). The exact combination of enzymes and reactions that occur differ depending on the part of the body that is being considered, and the function of the cell. For example, the cells in the brain may perform some of the same functions as cells in the liver, such as cell replication, while other functions are significantly different, resulting in the use of different enzymes.

The liver has a range of roles, including the maintenance of blood glucose during starvation by the activation of glycogen. It is a critical organ for survival (Salway, 2012). As well as variation in function and enzyme composition across organs and areas of the body, this also occurs within organelles present within cells. Determining what enzymes are present in different cellular components and their level of activity can be achieved through the use of cell fractionation techniques (Scnaitman and Greenawalt, 1968). One cellular component of interest is the mitochondria, which are essential organelles, involved in cell death pathways as well as the production of metabolic energy (van Loo et al., 2002). ...Download file to see next pages Read More
Cite this document
  • APA
  • MLA
(Malate Dehydrogenase Isoenzymes Lab Report Example | Topics and Well Written Essays - 1723 words, n.d.)
Malate Dehydrogenase Isoenzymes Lab Report Example | Topics and Well Written Essays - 1723 words. Retrieved from
(Malate Dehydrogenase Isoenzymes Lab Report Example | Topics and Well Written Essays - 1723 Words)
Malate Dehydrogenase Isoenzymes Lab Report Example | Topics and Well Written Essays - 1723 Words.
“Malate Dehydrogenase Isoenzymes Lab Report Example | Topics and Well Written Essays - 1723 Words”, n.d.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Malate Dehydrogenase Isoenzymes

Alcohol and Alcoholic Dietary Patterns

lism; liver function; NAD; fatty liver; oxidative stress; antioxidants; S–adenosylmethionine Introduction Background Information and thesis statement Alcohol provides various functions for people throughout history. It was used for a variety of purposes in the past and even now. Since the past times to the present, alcohol has played a crucial role in religion and worship. Alcoholic beverages have been used as sources of nutrients and also for their antiseptic, medicinal, and analgesic properties. Many people who take alcohol are malnourished, either because they take little of essential nutrients (e.g., proteins, carbohydrates, and vitamins) or because the metabolization of alcohol prevents the body from digesting, absorbing, and...
10 Pages (2500 words) Term Paper

Alcohol, cardiovascular disease and genetic modification

Thus, although the effects were inconclusive among lifetime infrequent drinkers, among former drinkers and among heavy drinkers, alcohol consumption seemed to decrease the risk of CVD among those who drink light to moderate amounts of alcohol. Despite the promise of alcohol consumption in the protection against CVD, future studies are derailed by the difficulty in conducting studies on the effects of alcohol and CVD, since dietary patterns, physical activity and smoking all affect volume and type of alcohol consumed and drinking patterns, aside from causing CVD directly. It must be noted that healthy behaviors decreasing the risk for CVD are associated with low to moderate alcohol consumption. These factors affecting alcohol inta...
9 Pages (2250 words) Research Paper

Errors of metabolism (newborn screening)

...? Topic: Errors Of Metabolism (Newborn Screening) Background of Disease Medium chain acyl Co A dehydrogenase deficiency (MCADD) is one of fatty acid oxidation disorder affecting fatty acid oxidation, thus preventing the conversion of fats to energy. Medium-chain acyl Co A dehrdogenase enzyme is involved in fatty acid metabolism, during times when glycogen reserves in the body have repleted. Mostly, this disease exhibits itself during infancy and childhood ( three months to two years). Considering the epidemiology , it is one of the most common fatty-acid disorder with an incidence of about 1 in 10,000- 20,000. (CPSP, n.d.) (Rinaldo, 1988) Various findings also suggets that this disease is genetically inherited via autosomal recessive...
4 Pages (1000 words) Essay

Clinical biochemistry and advanced assessment in nutrition

...?Other (s) Clinical Biochemistry and Advanced Assessment in Nutrition Similarities and differences between Pyruvate Dehydrogenase Complex and Branched Chain Ketoacid Dehydrogenase Complex There are a number of similarities and differences regarding the functionality and nutrient requirements of Pyruvate Dehydrogenase and Branched Chain Ketoacid Dehydrogenase enzyme complexes. Pyruvate dehydrogenase complex is an important enxyme complex that particularly works by transforming pyruvate into acetyl-CoA through a process known as pyruvate decarboxylation (Smolle 196). The resultant product (acetyl-CoA) is then normally used during the citric acid cycle to enhance cell respiration. Structurally and functionally, pyruvate dehydrogenase complex...
3 Pages (750 words) Assignment

Cellular Respiration

ATP is called as a "Universal energy currency" since all the energy transactions of the cell use this molecule.
In terms of efficiency, aerobic respiration is more efficient than the anaerobic respiration which yields only two molecules of ATP. Both these modes of respiration share the initial Glycolysis step, which occurs in cytoplasm. After, the glycolysis, other reactions of the aerobic respiration, Krebs (Citric acid cycle) and oxidative phosphorylation takes place in mitochondria.
Of the four different isozymes, type IV enzyme is not amenable to feedback inhibitory mechanism and is specific to liver and pancreas. During elevated levels of blood glucose, excess amount of Glucose-6-phosphate is made for conversion to g...
9 Pages (2250 words) Essay

Medical Biochemistry

... and research settings. Lactate dehydrogenase (LDH) is present in many human tissues, enabling cells to undergo anaerobic glycolysis; its main cellular function, conversion of pyruvate into lactate, provides the energy production cycle with more substrates. Normal and abnormal levels are now standardized, and measuring its activity in serum/plasma will help elucidate the origins of damage or disease. There are different isoenzymes of LDH, which differ in their structure and certain other properties. Because these different isoforms are found in distinct locations, its measurement is key when establishing the site of damage. In this practical work, which is divided into 3 weeks, we will first make a comparison of the absorption...
10 Pages (2500 words) Essay

Determination of Serum Lactic Dehydrogenase Activity

... different. In the case of haemolysed red blood cells, the levels of LDH would be higher as all the LDH would have leaked out of the red blood cells. 5) Human’s have 5 different types of isoenzymes of LDH. LDH-1 has 4 H subunits. LDH-2 has 3 H subunits and 1 M subunit. LDH-3 has 2 H subunits and 2 M subunits. LDH-4 has 1 H subunit and 3 M subunits. LDH-5 has 4 M subunit. The difference in the combination of sub units affects the stability of the enzymes. 6a) The different LDH enzymes can be separated by electrophoresis because each LDH isoenzyme is made up of different combinations of H and M subunits or only H or M subunits. And each combination produces a LDH with a different molecular weight. Due to this we can separate the different...
3 Pages (750 words) Essay

Alkaline phosphatase enzyme

...ALKALINE PHOSPHATASE ENZYME Introduction Alkaline Phosphatase is a group of enzymes that is primarily found in the liver (isoenzyme ALP and bone (isoenzyme ALP-2). These enzymes are also produced by cells coating the intestines (isoenzyme ALP-3), the placenta, and also the kidney (in the proximal convoluted tubules). The test of Alkaline Phosphatase in the body measures the total amount of the enzymes that are released from the tissues of the above mentioned parts in to the blood. Due to this, the alkaline phosphatase enzyme works best at an alkaline pH of 10. As a result, the enzyme is inactive in the blood. The enzyme acts by splitting off phosphorous creating an alkaline pH. Function Alkaline phosphatase enzymes are phosphate...
5 Pages (1250 words) Essay

The genetic basis of glucose-6-phosphate dehydrogenase (G6PD) deficiency

...The Genetic Basis of Glucose-6-Phosphate Dehydrogenase (G6PD) Deficiency Introduction Among human enzyme defects glucose-6-phosphate dehydrogenase (G6PD) deficiency is the most frequently seen. G6PD deficiency affects more than four hundred million people around the world. Its prevalence around the world has marked similarity to that of malaria, which has led to the malaria protection hypothesis with regard to its prevalence. The malaria parasite is found to have a bias in its preference for the G6PD normal red blood cells. This preference gives rise to the malaria protection hypothesis (Capellini & Fiorelli, 2008). Capellini & Fiorelli 2008, pp.64, define G6PD deficiency as “an X-linked, hereditary genetic defect due to mutations...
4 Pages (1000 words) Essay

Strategic Planning of Any Company

...CONTENTS Introduction Page Macro Analysis Page 2 PESTLE Analysis Page 3 Five Factors Page 7 Strategic Choice Page 7 Strategic Implementation Page 8 STRATEGIC ANALYSIS INTRODUCTION Planning is an inevitable part of every corporate firm on which all its functioning stands. All the organizations either large or small devise methods and apply techniques... Introduction Page ...
11 Pages (2750 words) Coursework

The Experiences of Boys and Girls in the Context of a Romantic Relationship

...Relationships and Connections of Variables in the Research Study The literature offers a myriad of information yielded by various researches on humanrelationships. Concepts on a wide spectrum from physiological responses to romantic relationships, emotions that are manifested during romances, social implications or consequences of engaging in romantic relationships, and perceptions and viewpoints that are attempted to explain some phenomena related to romance. Studies on romance include the studies of social scientists who unearthed evidence of physiological responses manifested by heightened hormones in new romances (ex. the researches of Dr. Donatella Marazitti (Kahn, 2004) and Helen Fisher (Fisher, 2004). Adolescents, who... and...
17 Pages (4250 words) Case Study

Characteristic of Murder Scenes in Literature

...The following work analyzes two murder scenes, one of which is extracted from chapter forty-five of Perfume, a novel written by Patrick Süskind, and the other from chapter eleven of Thérèse Raquin, one of Emile Zola’s most famous literary works. In Perfume, Grenouille, the main character, murders a young girl in order to extract her scent which forms the ultimate ingredient required to successfully create his exquisite perfume. The enchanting odour of this perfume would serve Grenouille’s goals as it would make him hypnotize people.On the other hand, in Thérèse Raquin,Thérèse ,an adulterous wife, gives her consent to Laurent, her lover, to kill her own husband,Camille,as he’s the osbtacle standing against their mariage... following work...
6 Pages (1500 words) Case Study

The Existence of Plate Tectonics on Mars

It is suspected that Mars may have had an atmosphere, water system, and crust similar to that of Earth. However, observations of the planet’s current state show Mars at a much higher level of deterioration that that of Earth. NASA’s latest Mars project, Phoenix, made a successful landing on Mars and spent five months collecting data that has yet to be fully analyzed on the planet’s climate, soil, and atmosphere. This mission yielded further proof that there was a type of water on or near the surface of Mars. Soil samples collected prove that there could have once been a sustainable life on the planet (Cowing). There have also been findings in recent years suggesting that Mars once had a molten inner core, much li...
9 Pages (2250 words) Research Paper

Learning and Memory Process

...Profile Report This report compares and contrasts the answers provided by the interviewees X and Jason. Learning and memory process Interviewee X prefers to learn by observation and has the ability to give directions to a place that X has earlier visited. Jason also prefers to learn by observation. Interviewee X prefers to work in silence to avoid distraction whereas Jason prefers distractions as they force him to concentrate. Quietude makes his mind wander. Interviewee X’s preference to work in silence points to the use of committing work to the long term memory. Jason displays a preference to utilize short term memory for his work since it has the capacity to focus on the work at hand despite distractions around. The use... Report This...
6 Pages (1500 words) Assignment

Why and How the Negative Opinions on Boxing Are Justified

...Research paper on why and how the negative opinions on boxing are justified Introduction: Sporting activity is essentially an offshoot of the primordial instinct of survival and growth of a society. Over the years it has evolved into many forms ranging from direct physical confrontation to indirect acts like using balls, racquets, and balls. But one sporting activity that is still reminiscent of the primordial instinct is boxing. Even though it has developed a set of rules and regulations that sets it apart from Plain Street fighting, there are concerns that it is too violent, and also that it promotes violence and is detrimental to long term health of the participants. This paper reviews the relevance of boxing in today’s world... paper on...
7 Pages (1750 words) Assignment

Cultural Communication

...CULTURAL COMMUNICATION SELF-ASSESSMENT PAPER Cultural communication is a very interesting to analyze in light of globalization and the interconnectedness of different cultural communities throughout the world. Evaluating one’s own cultural background is difficult to do from an objective standpoint. It is also even more difficult to understand how one’s cultural background affects our communication styles with people from our own culture as well as with people from other cultures. With the aim of understanding cultural communication fully, the following will provide a nice overview of my own cultural background. Following this, I will explain the communication style of my culture and then look at how my cultural background... COMMUNICATION...
7 Pages (1750 words) Essay

Validating Late Mover Advantages in e-commerce: Evidence from Selected Companies

...Research Proposal: Validating Late Mover Advantages in e-commerce: Evidence from Selected Companies Table of Contents Table of Contents 1 Title 3 Background 3 Objective 4 Project Aim 4 Research Hypothesis 5 Research Questions 5 Ethical Considerations 6 Literature Review 6 Research Methodology 9 Phenomenological Paradigm 9 Positive Paradigm 9 Deductive vs. Inductive Research 10 Data Collection Techniques 10 Proposed Research Methodology 11 Primary Data Collection 11 Survey Questionnaire 11 Research Methodology Limitations 12 Proposed Structure of Report 13 Time Schedule 16 References 17 Title Validating Late Mover Advantages in e-commerce: Evidence from Selected Companies Background E-commerce is a global market place... Proposal: Validating...
12 Pages (3000 words) Research Proposal

Enzymes Involvement in Microbial Technology: Biotechnological Operations

Elevated Serum amylase was first associated with pancreatitis in 1908. Since then diagnosis enzymology has grown rapidly (Wohlgemuth, 1908). Yet several considerations are needed to make a sensible interpretation of serum enzyme determination. Increased serum enzyme activity is mostly related to enzymes released from injured cells. The elevated activity occurs to the same enzyme which is abundantly present in the injured organ (Karmen et al., 1954). The time course of the increase in activity of the enzyme in serum is analogous to the time course of decrease in enzyme activity from a damaged organ (Jennings et al., 1957). However, the highest elevation of serum enzyme activity does not have to be proportional to the magnitude of c...
11 Pages (2750 words) Literature review
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic Malate Dehydrogenase Isoenzymes for FREE!

Contact Us