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Malate Dehydrogenase Isoenzymes - Lab Report Example

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The lab report "Malate Dehydrogenase Isoenzymes" points out that This experiment examined the localization of three enzymes within liver cells. The enzymes being examined were malate dehydrogenase (MDH), lactate dehydrogenase (LDH) and succinate dehydrogenase (SDH)…
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Malate Dehydrogenase Isoenzymes
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Download file to see previous pages The activity of protein also varied between the fractions, with the liver particulate and lysed liver particulate showing higher levels of protein than the supernatant. The results of the experiment indicate that the majority of energy converting reactions is centered within the mitochondria, with the MDH reaction causing the highest amount of absorption, suggesting a stronger reaction. The prevalence of protein within this portion of the cell supports this hypothesis. The use of electron acceptors to measure the strength of reactions was an effective approach to determining the prevalence of each reaction, as well as their localization within the cell.

Throughout the human body, different enzymes interact with one another to form comprehensive reactions and chains of reactions that play important roles in the functioning of the human body (Schilling et al., 1999). The exact combination of enzymes and reactions that occur differ depending on the part of the body that is being considered, and the function of the cell. For example, the cells in the brain may perform some of the same functions as cells in the liver, such as cell replication, while other functions are significantly different, resulting in the use of different enzymes.

The liver has a range of roles, including the maintenance of blood glucose during starvation by the activation of glycogen. It is a critical organ for survival (Salway, 2012). As well as variation in function and enzyme composition across organs and areas of the body, this also occurs within organelles present within cells. Determining what enzymes are present in different cellular components and their level of activity can be achieved through the use of cell fractionation techniques (Scnaitman and Greenawalt, 1968). One cellular component of interest is the mitochondria, which are essential organelles, involved in cell death pathways as well as the production of metabolic energy (van Loo et al., 2002). ...Download file to see next pages Read More
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