quora
Nobody downloaded yet

Measuring the reactions of the enzymes catecholase in different levels of pH in different tempereture - Lab Report Example

Comments (0) Cite this document
Summary
These enzymes have active sites which bind to the substrates thereby converting the substrate into the final products at a faster rate due to the lowering of the energy of activation. Enzymes are however…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.7% of users find it useful
Measuring the reactions of the enzymes catecholase in different levels of pH in different tempereture
Read TextPreview

Extract of sample
"Measuring the reactions of the enzymes catecholase in different levels of pH in different tempereture"

Download file to see previous pages Temperature rise of about 10oC normally doubles or triples the rate of enzymatic reactions. However beyond the optimum temperature the enzyme activity decreases. If catecholase activity is minimally affected at different temperatures and pH then it can be hypothesized that it has a large range of optimal activity. The aim of this experiment was to test the effect of temperature and pH on the enzymatic activity of catecholase.
The effect of catecholase enyme at different temperatures, 0 and 37oC were tested at different levels of pH 2, 4, 7 and 12. Three tubes for each pH were set up and labelled A, B and C. To three tubes in each pH 3ml of the pH solution was added and 2ml of banana extract (substrate). To two tubes 1ml of the enzyme (potato extract) was added while the third tube the enzyme was not added and acted as the control. The test tubes were then shaken and put in the appropriate temperature and the optical density measured after three minutes.
The reaction rate was the highest at all temperatures at pH 7. At room temperature the reaction rate was the highest in the tube without the enzyme. pH 4 and 12 had the lowest reaction rates at all temperatures. The reaction rates were highest between 0oC and room temperature in the presence of catecholase and lowest at 0oC without the enzyme.
Catechol oxidase (catecholase) is a polyphenol oxidase enzyme present in many plants (Aniszewski et al. 2008). It is responsible for catalysing the reaction between oxygen and hydroxylated benzenes leading to the production of quinines and water. The enzyme mediated reaction rate was the highest at physiological pH and at temperatures between zero and room temperature. This shows that the enzyme has a large range of optimal temperatures. However, it has a narrow range of optimal pH. The narrow pH range during which catecholase activity was the highest is due to the effect of pH on the redox ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Measuring the reactions of the enzymes catecholase in different levels Lab Report”, n.d.)
Retrieved from https://studentshare.org/biology/1599557-measuring-the-reactions-of-the-enzymes-catecholase-in-different-levels-of-ph-in-different-tempereture
(Measuring the Reactions of the Enzymes Catecholase in Different Levels Lab Report)
https://studentshare.org/biology/1599557-measuring-the-reactions-of-the-enzymes-catecholase-in-different-levels-of-ph-in-different-tempereture.
“Measuring the Reactions of the Enzymes Catecholase in Different Levels Lab Report”, n.d. https://studentshare.org/biology/1599557-measuring-the-reactions-of-the-enzymes-catecholase-in-different-levels-of-ph-in-different-tempereture.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
The Effects of Temp & PH on Enzymes
This is because they have unique active sites into which only specific substrates can fit. Enzymes bind temporarily to one or more of the reactants of the reaction they catalyze and in doing so; they lower the amount of activation energy needed and thus speed up the reaction (Groves, 1997).
6 Pages(1500 words)Lab Report
PH Titration
Acid-base titrations are applied in order to estimate the molarity either of an acid or a base used through a setup that consists of a burette that holds the ‘titrant’ and flask filled with ‘analyte’ or the solution with unknown concentration.
6 Pages(1500 words)Lab Report
Enzymes activity
Inhibitors also affect working of enzyme by competitively, non-competitively and mixed reaction. Method: a calibration graph that had been previously prepared was used. A Line-weaver Burk was plotted with reciprocal of substrate plotted against reciprocal of
7 Pages(1750 words)Lab Report
PH Indicators
5 Pages(1250 words)Lab Report
MEASURING pH
Most measured substances were already in the liquid state. However, lemon juice was diluted prior to the measurement while the Rolaid antacid tablet was crushed and mixed in water prior to measurement. For the first titration
2 Pages(500 words)Lab Report
Ph conductivity lab
Using this approach, the concentration of the NaCl solutions could be directly determined. The PH of various solutions was also measured using an electronic PH probe, red
4 Pages(1000 words)Lab Report
Enzymes
The composition of enzyme is primarily a complex protein molecule and function by catalyzing specific biochemical changes within the bodies of animals and plants such as digestion of food in animals and photosynthesis in plants. Enzymes acts on substrates and
8 Pages(2000 words)Lab Report
Catecholase, Enzymatic Browning and Temperature
Enzymes are biochemical catalysts for chemical processes in the cell by increasing the rate of reaction and lowering the activation energy. Substrates bind to the active sites of the
4 Pages(1000 words)Lab Report
Enzymes lab report
The results show that lactase activity is low when temperature is low at 00C but improves with increasing temperature up to 370C after which activity drops sharply. Low activity at low temperature is due to low activation energy exhibited by
5 Pages(1250 words)Lab Report
PH Titrations
A standard solution is defined as a reagent. In most cases, a concentration that is known reacts with the titrant volume to establish the concentration. Titration involves a group of experiments in which the known solution property is utilized in inferring to the unknown property of a different solution.
4 Pages(1000 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Measuring the reactions of the enzymes catecholase in different levels of pH in different tempereture for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us