The purpose of this lab report is to investigate the effect of temperature and pH on the activity of catalase. It is hypothesized that various temperatures would influence the activity of catalase. It is also hypothesized that various pH values would affect the activity of catalase…
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The researcher states that enzymes are protein molecules with a three-dimensional shape that includes an active site whose shape is complementary to the shape of the substrate. For this reason, each enzyme is specific to the type of substrate and reaction that it can catalyze. Several factors affect the rate of enzyme activity. They include temperature, enzyme concentration, substrate concentration, and pH. Enzymes, being made of proteins, are sensitive to factors that affect the structure and function of proteins. Therefore, each type of enzyme has an optimum range of temperature and pH at which it exhibits maximum activity. Any deviation from the optimum pH of an enzyme leads to interactions of the charged groups of the amino acids in the active site of the enzyme, which leads to a distortion of the enzyme active site that consequently lowers catalysis. For example, the enzyme catalase is responsible for the breakdown of hydrogen peroxide into oxygen and water. Therefore, the amount of oxygen produced is proportional to the activity of catalase. Hence, it is important to measure the amount of oxygen produced to measure the activity of catalase. Three test tubes were filled with 4ml of catalase and 2ml of distilled water while three other test tubes were filled with 5ml of hydrogen peroxide. The tubes containing peroxide and catalase were labeled as 2oC, 37oC, and 80oC. A plastic tube was then filled with cold tap water to an approximate depth of 3 to 4 inches after which the temperature of the water was adjusted to between 37 and 40oC using warm water. 50ml graduated cylinders were then prepared by submerging them in the water tubs and allowing them to fill without any air bubbles. The cylinder was then put upside down with its mouth submerged. The cylinder was then put on a ring stand and secured in position with the mouth just below the surface of the water.
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The researcher utilized Lactaid which is an over the counter tablet as a source of enzymes, known to particularly digest protein and pectin. Obviously, the research adopted the experimental method. So, a control and an experimental set-up were installed for observations.
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The author states that combination of inorganic fertilizer and organic fertilizer provides efficient environment for the growth of soil bacteria hence production of more alkaline phosphatase enzymes. These findings shows how an integrated fertilizer regime play an important role in altering the structure of the soil microbial composition.
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The extent of hydrolysis was based on the time of reaction. The product is glucose if starch was completely hydrolyzed. The absence or presence of starch in the solutions was tested using iodine. Iodine never reacts with glucose, instead, forms a blue to black complex with starch.
5 Pages(1250 words)Lab Report
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