quora
Nobody downloaded yet

Catecholase, Enzymatic Browning and Temperature - Lab Report Example

Comments (0) Cite this document
Summary
This leads to formation of brown pigments that cause loss of quality and deterioration of crops after harvesting (Chi, et al., 2014). The enzymatic activity causes…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER96.1% of users find it useful
Catecholase, Enzymatic Browning and Temperature
Read TextPreview

Extract of sample
"Catecholase, Enzymatic Browning and Temperature"

Download file to see previous pages The rate of enzyme reaction is affected by temperature, substrate concentration, pH, and presence of inhibitors and cofactors. The equilibrium model describes the effect of temperature on enzymes. In this model, enzymes lose activity at high temperatures and low and at low temperatures (Peterson, Daniel, Danson, & Eisenthal, 2007).
The main hypothesis is to find out the effect of temperature on the rate of reaction and absorbance of enzyme catechol. To test the hypothesis, a solution of potato extract and deionized water was heated to four different temperatures and the rate of reaction and the absorbance was determined.
It was found out that when the solution of the potato extract and deionized water and catechol was heated at room temperature, the measure of absorbance increased until at 40 °C. The absorbance value then reduced when the solution was heated at 60 °C to 100 °C.
In enzymatic reactions, increase in temperature leads to increase in the rate of reaction due to the additional heat that increases random molecular movement. The activation energy of the reaction is thereby affected due to stress in the molecular bonds caused by the movement. From the findings, absorbance increased from room temperature to 40°C –the optimum temperature. Most enzymes have an optimum temperature between 35 °C and 40 °C. At room temperature, the hydrophobic interactions and the hydrogen bonds were not flexible enough to induce fit that was optimum for catalysis. At 60 °C, the forces are too weak to maintain the enzymes shape against the increased random movement of the atoms in the enzyme. At boiling temperatures the enzyme denatures and does not take part in chemical reactions effectively. This is in consistent with findings by (Daniel, et al., 2009)
The main limitation for this experiment is that there was a delay of 10 seconds in pressing the button on the calorimeter. Therefore the total time used ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Catecholase, Enzymatic Browning and Temperature Lab Report”, n.d.)
Retrieved from https://studentshare.org/biology/1694666-catecholase-enzymatic-browning-and-temperature
(Catecholase, Enzymatic Browning and Temperature Lab Report)
https://studentshare.org/biology/1694666-catecholase-enzymatic-browning-and-temperature.
“Catecholase, Enzymatic Browning and Temperature Lab Report”, n.d. https://studentshare.org/biology/1694666-catecholase-enzymatic-browning-and-temperature.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Temperature Effect
According to the author of the text, the temperature input significantly affects the mean output of the amylase hydrolysis laboratory experiments because temperature affects the number of enzyme collisions. In addition, trend analysis indicates that as the temperature increases at zero time period, the mean output increases.
9 Pages(2250 words)Essay
Biology lap report
Hydrolysis of starch is executed by amylase, an enzyme that acts on starch to break it down into maltose, maltriose, short oligosaccharides. This paper determines the enzymatic activity of amylase over a range of temperature and thus
4 Pages(1000 words)Lab Report
Temperature Monitor Simulation Using LabVIEW
As can be seen in the block diagram created using LabVIEW (Fig. 1), a Dial control was used as the source of the temperature signal. The signal was passed through Multiply and Add functions to convert it from a Celsius value to a Farenheit equivalent. A Waveform Chart was used to display temperature variation with time.
20 Pages(5000 words)Lab Report
The effects of enviromental temperature on human respiration
This also raises the risk of heart attack. According to Sun, a professor at Florida University, “Everyone should bear in mind that cold
3 Pages(750 words)Lab Report
Enzymatic Analysis of Yeast Alcohol Dehydrogenase
The first experiment measured the effect of time on the recovery by stopping the reaction at intervals of 5 minutes. Maximal values of MTT formazan, at an absorbance of 570 nm were obtained at 15-30
3 Pages(750 words)Lab Report
Measuring the reactions of the enzymes catecholase in different levels of pH in different tempereture
Many enzymes work well in a narrow range of pH. A change in pH may alter the three dimensional structure of the enzyme due to formation or breakage of covalent bonds within the enzyme. A change in the structure
1 Pages(250 words)Lab Report
An Effect of an Acidic Fluid on Enzymatic Activity

According to the research, enzymes are the chemical molecules made of proteins and catalyze various biological reactions for the survival of all living organisms on the planet. They are highly specific to the conditions in their vicinity. Any alteration in the conditions, make them inactive as modified conditions alter the shape of the protein molecules.

3 Pages(750 words)Lab Report
As temperature increases the level of turbidity increases
Nutrients dissolved in it determine survival of aquatic organisms not to exclude turbidity, these mentioned factors greatly determine the survival of aquatic organisms. Millan, Ashly Suggests that turbidity is the measure of the extent of
1 Pages(250 words)Lab Report
Rate of product formation in an enzyme - catalyzed reaction
The optimum pH range was found to be 5-6 and the given enzyme does not work at all in the pH 3. Enzymes are protein molecules present in all the living cells. They act as biological catalyst for speeding up the reactions by lowering the energy barrier. Enzymes are made
5 Pages(1250 words)Lab Report
Enzymatic Activity
It is good to keep in mind that a catalyst is a substance that lowers the activation energy needed for a chemical reaction to take place. Above are the main materials used in this experiment where the
2 Pages(500 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Catecholase, Enzymatic Browning and Temperature for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us