Catecholase is present in fruits like apple, eggplant, and potato. When the potato is cut, catecholase convert the catechol present in potato into benziquinones, an antiseptic in plants. This reaction occurs at a…
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Each and every protein has a specific site or substrate binding and this site remains active at specific pH, temperature and concentration of the substrate. The enzyme active site are specific for the substrates, if some other substrate comes in contact with the enzyme, there will be no ligand – receptor complex (Gurung et al., 2013).This is carried out by the ligand – receptor complex. The enzyme bound to its substrate, forming enzyme- substrate complex, which became activated. At the end of the reaction, the product is released and the same enzyme is ready to react with other substrate. The enzymes are affected by many external parameters such as pH and Temperature. The alteration of the active site occurs due to the change in pH and temperature. The protein gets denatured and loses its active site. Finally the enzyme activity is lost (Gurung et al., 2013).
Catecholase is an enzyme present in plants. Catecholase catalyses oxidation of catechol. Catechol is found behind the skin of many fruits such as apples, potatoes. Catechol molecules on exposure to air, gets oxidized into benzoquinone. Benzoquinone is a brown color substance and changes the solution color to brown. Benzoquinone thus formed inhibits the growth of the micro organisms in the fruits. Catecholase oxidase catalyzes the oxidation of the aromatic group with adjacent phenol groups. This enzyme requires copper ions for the activation. The activity of the enzyme depends on temperature, pH and substrate concentration. The enzyme structure and activity is dependent on the active site and variable groups.
The catecholase catalyzed reactions were tested with the given sample. Two test tubes were taken and to each of them 1 ml of potato extract was added. To the test tube named B 2ml of deionized water was added and to the test tube C 1 ml of deionized water was added. To B test
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The researcher utilized Lactaid which is an over the counter tablet as a source of enzymes, known to particularly digest protein and pectin. Obviously, the research adopted the experimental method. So, a control and an experimental set-up were installed for observations.
It proved that high enzyme concentrations and optimum pH levels and temperatures increased enzyme activity. Introduction: Amylase is an enzyme that helps in breaking down starch to form sugars such as maltose and glucose. In human beings it is found in the saliva.
In general terms this was to introduce concepts related to enzyme kinetics. Introduction Enzymes are among several proteins generated in living cells to catalyze or accelerate the organism’s metabolic processes. Normally, enzymes are so selective and specific on the molecules they catalyze, referred to as substrate (Dixon, 1979).They normally do react specifically with a single substrate.
The author provides an experiment, which has 1 independent variable, 1 dependent variable and 1 control variable. The independent variable is the various concentrations of hydrogen peroxide, while the dependent variable is the rate of reaction in terms of oxygen production. The control variable is the temperature of the room and standard pressure.
There are certain factors that influence the activity of catalase:
• Substrate Concentration
• Salt Concentration
• Presence of Inhibitors
• Presence of Activators
Hence even if the same experiment is performed several times different results may lead to different results.
As an anion, thiosulfate is tetrahedral in shape and is depicted by replacing one of the oxygen atoms by a sulfur atom in a sulfate anion. The S-S distance suggests a single bond, an indication of a significant negative charge borne by the sulphur
hrough the method of extracting the enzyme by blending it with potato tissue in phosphate buffer, the effects were analyzed on the effect of the dye guaiacol and the activity measured under different temperatures. The optimum temperature was obtained at 22.20C and above this
According to the author of the report, enzymes are capable of regulating the rate at which chemical reactions occur without being altered in the process. To be precise, enzymes achieve this function by lowering the activation energy necessary for the reaction to occur in cells.