StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

The Effect of Temperature on Enzymatic Activity of Amylase - Lab Report Example

Comments (0) Cite this document
Summary
This paper determines the enzymatic activity of amylase over a range of temperatures and thus brings a close look at the optimal temperature for amylase activities in humans (human amylases) and fungus, Aspergillus oryzae (fungal amylase). The temperature range was set for 0°C to 95°C. …
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER96.6% of users find it useful
The Effect of Temperature on Enzymatic Activity of Amylase
Read TextPreview

Extract of sample "The Effect of Temperature on Enzymatic Activity of Amylase"

Download file to see previous pages The rate of reaction was directly proportional to the increase in temperature, but there was a decline after 40°C as envisaged in the hypothesis, the enzymes denature with a very high elevation in temperature, concluding that if the temperature is increased beyond the optimal temperature then the enzymatic activities come to a close and enzyme becomes denatured. The study also envisaged that fungal amylase does display the slightly faster hydrolytic action on starch as compared to the human salivary amylase.

Starch is a regular component of our daily diet. It is perhaps an extensive carbohydrate in the plant world and is consumed by humans and other herbivores to meet their energy requirements. It is a polymer of glucose which depicts the linkage of α-1,4 and α-1,6 glucosidic bonding. It is essential to break this bonding to derive carbon and energy from the starch molecules; this task is performed by a group of enzymes called amylases (Lehninger, 2008).

There are various organisms that serve as sources of amylase, human saliva, and pancreas, other organisms encompass plants fungi and bacteria. The present study includes two groups of amylases, human amylase, and fungal amylase. Human amylase was obtained from the saliva while the fungal amylase was procured from Aspergillus species which is highly prevalent and generally acquired from the soil, putrefying foliage and also present in the air.

Any reaction needs some initiation energy for its onset; this is also true for biological processes. Hydrolysis of starch also requires some activation energy but due to the presence of biological catalysts, enzyme amylase this activation energy can be reduced and the reaction is carried out with ease. Enzymes are extremely specific for substrates, pH, temperature.  ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(The Effect of Temperature on Enzymatic Activity of Amylase Lab Report, n.d.)
The Effect of Temperature on Enzymatic Activity of Amylase Lab Report. Retrieved from https://studentshare.org/biology/1558249-biology-lap-report
(The Effect of Temperature on Enzymatic Activity of Amylase Lab Report)
The Effect of Temperature on Enzymatic Activity of Amylase Lab Report. https://studentshare.org/biology/1558249-biology-lap-report.
“The Effect of Temperature on Enzymatic Activity of Amylase Lab Report”, n.d. https://studentshare.org/biology/1558249-biology-lap-report.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF The Effect of Temperature on Enzymatic Activity of Amylase

Enzyme Amylase Lab Report

...?The effects of pH, temperature and enzyme concentration on enzyme activity This experiment aimed to prove that the activity of amylase is affected by parameters such as temperature, pH and concentration. It measured how changing levels of the parameters led to changes in enzyme activity. It proved that high enzyme concentrations and optimum pH levels and temperatures increased enzyme activity. Introduction: Amylase is an enzyme that helps in breaking down starch to form sugars such as maltose and glucose. In human beings it is found in the saliva. An enzyme is a protein-based catalyst that raises the reaction rate of a reaction by lowering the activation energy needed for that reaction. It is not used up in the process. It is hypothesized...
5 Pages(1250 words)Lab Report

Effect of Concentration and Temperature on the Rate of Reaction

...Effect of Concentration and Temperature on the Rate of Reaction Aim To see the reaction between Methanoic acid and Bromine Apparatus Measuring Cylinder, burette, gas syringe, volumetric flask Method Add the acid into the measuring cylinder and measure to the amount needed for the reaction. Add the amount into the burette and now from burette add the acid gradually in the volumetric flask. On the other side, we will compress the bromine gas into liquid and add it to the Methanoic acid through pipette. Introduction Reactions in nature take place at diverse speeds. Some reactions are incredibly slow, like the erosion of rocks, while other reactions can be extremely fast like the burning of wood. The speed of a reaction is at times vital...
5 Pages(1250 words)Essay

The temperature

...The TemperatureTemperature is the heat of an object, measured by its intensity or degree on a defined scale” (Elizabeth, . A gas constitutes molecules that travel in different directions with different velocities. Some molecules move faster as compared to the others (Cool Cosmos, n.d.). Temperature of a substance measures the average thermal energy or heat of its atoms and and/or molecules. Planck temperature is considered to be the maximum attainable temperature (Anissimov, 2011). There are numerous forms of energy including heat energy, gravitational energy, light energy, electrical energy, mechanical energy, nuclear energy, chemical energy and sound energy. Energy can transform from one form to another and it can also be transferred...
2 Pages(500 words)Essay

Effect of Temperature on Peroxidase Activity

... Temperature affects the enzyme activity as it changes the conformation of the enzyme. Enzymes are proteins which are very sensitive to temperature changes. Peroxidase is an enzyme that is present in all animals, higher plants and aerobic cells. Peroxidase is required by the cell to remove the toxic hydrogen peroxide released by the biochemical reactions. Peroxidase is an oxidoreductase enzyme that reduces H2O2 into water and releasing oxygen for the oxidation of the organic compounds. The reaction rates of the enzyme at different temperatures were determined in this experiment. It was found that the enzymatic activity was optimum at 37 degree Celsius and the enzyme activity decreased above that temperature thus nullifying the hypothesis...
8 Pages(2000 words)Essay

An Effect of an Acidic Fluid on Enzymatic Activity

...Aim: To design an experiment in order to test the effect of an acidic fluid on enzymatic activity. (Recall: Enzymes are proteins). Laboratory Materials: Materials in the laboratory kit. Litmus paper to check the pH of the solution. Plastic beakers or test tubes, peptone, sample of fresh meat, about 1 cm cubed in size, (this should be unprocessed and uncooked). For instance pieces of liver, streak, fish or poultry. Sample of 1 cm cubed fresh vegetable. For instance potatoes or other root vegetables. A marker or pen for labelling. A ruler. Theory Enzymes are made up of protein molecules. They catalyze biochemical reactions. The enzyme catalyzes the reaction where the reactants are enzyme and the substrate. Substrates combine at an active...
3 Pages(750 words)Lab Report

Enzymatic Activity

... the greatest absorbance. It was notted that the higher the temperatures, the more the enzymatic activity. In the cofactor procedure, the PTU differed with the EDTA where the previous showed the smallest amount in increase regarding measurements while the latter appeared to have inhibited the enzyme reaction unlike the previous one. Discussion The hypothesis that the cuvette with both enzyme and catechol solution got placed in the 37 degree water. After adding the water, the solution formed requires filtration where after the solution is safely stored in a container. In conclusion, Several things could be done to improve the experiment including keeping the test tubes much further apart from each other for the purposes of preventing any...
2 Pages(500 words)Lab Report

The effect of salt temperature on ice

...The effect of salt on the temperature of ice Introduction Ice is formed when the temperature of water reaches 00c which is the freezing point of water. When salt is added to it, it acts as an impurity thus causing the temperatures to drop. It has been proven through laboratory experiments that a 10 percent salt solution freezes at -60c and a 20 percent solution freezes at -160C (Smith et al). The salt dissolves into the liquid water in the ice and lowers its freezing point. Ice which is in solid form and crystalline in nature formed through weak partial polar forces between the water molecules that are referred to as hydrogen bonds. These bonds hold the molecules together forming the solid ice (Churchill et al). The salt ions dissolved...
3 Pages(750 words)Essay

Temperature Effect

...Effect of Temperature on Amylase Hydrolysis of both Fungial Aspergillus_Oryzae and Bacterial Bacillus_Lichenifornis at Different Time Periods Professor Student Name: February 23, 2015 Group Member: (2) Abstract A laboratory experiment was conducted. The enzyme experiment focused on amylase hydrolysis from fungial Aspergillus_Oryzae and amylase hydrolysis from Bacterial Bacillus_Lichenifornis using starch hydrolysis. The procedures centered on both fungal amylase and bacterial amylase on four different temperatures levels and different time periods. Amylase hydrolysis includes soluble maltose (sugar) procedures. The findings indicate the temperature affects...
9 Pages(2250 words)Essay

Catecholase, Enzymatic Browning and Temperature

The rate of enzyme reaction is affected by temperature, substrate concentration, pH, and presence of inhibitors and cofactors. The equilibrium model describes the effect of temperature on enzymes. In this model, enzymes lose activity at high temperatures and low and at low temperatures (Peterson, Daniel, Danson, & Eisenthal, 2007).
The main hypothesis is to find out the effect of temperature on the rate of reaction and absorbance of enzyme catechol. To test the hypothesis, a solution of potato extract and deionized water was heated to four different temperatures and the rate of reaction and the absorbance was determined.
It was found out that when the solution of the potato extract and deionized water and catechol was heat...
4 Pages(1000 words)Lab Report

Greenhouse Effect

Approximately a third of it is reflected back into space. Specific atmospheric gases serve in much the same way as does the glass of a greenhouse, thus the terminology. These gases allow sunlight to penetrate then trap some of the solar energy which heats the earth (Breuer, 1980). It is a delicate balance and because these greenhouse gases have been artificially augmented by man-made sources, more build up in the atmosphere has occurred thus trapping more of the sun’s energy and reflecting less back into space. This occurrence is causing the earth to warm.
Carbon Dioxide (CO2) is the most prevalent of the greenhouse gases. Trees absorb CO2 and when they die, CO2 is restored to the atmosphere. The clearing of forests by...
6 Pages(1500 words)Assignment

Vietnam War Effect on Youth of America

The USA got involved in the Vietnam War as an ally of southern Vietnam.
Southern Vietnam was fighting against communist-led northern Vietnam and it was quite like a civil war. When the USA stretched itself into this civil war the soldiers were hard to motivate as they were bereft of patriotism, which they would have shown if it was a war directly against the USA, and fought to save national interest. After reaching Vietnam they felt more uncomfortable due to the tropical climate and the unknown topography. (Herring, 1979)

Their body kept on fighting while their mind got occasionally lost in their motherland in the arms of their beloved. Each war leaves a scar on the mind of a soldier and this scar is much deeper th...
8 Pages(2000 words)Assignment

Critique of Technological Innovations, Activity Based Costing and Satisfaction Article by Davood Askarany

Many of the innovations have been introduced as early as 1980, such as “activity-based costing (ABC); activity management (AM) and activity-based management (ABM); local information system (LS); balanced scorecard (BS); life cycle costing (LCC) and target costing (TC) [and] strategic management accounting (SMA).” (Askarany, Smith and Yazdifar 54). However, few of these have been implemented and utilized to their full potential. The authors propose to find a reason for this.

ABC focuses on costs associated with activities, but also evaluates whether those activities add value, thus providing a means of understanding how to most effectively reduce costs. In addition to cost reduction benefits, applying ABC giv...
7 Pages(1750 words)Term Paper

The Effect of Mass Media on Latinos' Self Image

The willful perversion of facts relating to the representation of Latinos in the U.S. media is disgraceful, inconsiderate and extremely misleading and hence calls for immediate attention to prevent the degradation of a particular section of the society since their depiction and imagery in mass media plays a significant role in shaping their social identity and governs their self-esteem. Television and films both being dominant forms of media persistently depict racial stereotypes which are largely false representations of the
According to U.S. Census (2000), Latinos are the fastest-growing ethnic communities in the United States constituting almost 12.5% of the population of which merely 3 – 4% of the Latino population...
7 Pages(1750 words)Term Paper

The Effect of Democracy on Chinese Development

Any country first needs to go through economic liberalization and political liberalization before finally settling into a stable democratic situation. Democracy should not at any one point be viewed as if it were socialism, but should be seen as a means of promoting economic development through accountability and protection of individual lawful rights and interests...accountability in the sense that the respective leaders are able to fight corruption, enhance social harmony, strengthen public trust and more importantly empower government institutions that are directly in touch with the people (Liu, 2008, p.1-2). Owing to the Chinese well informed and affluent society on top of having a very dynamic economy, the political change wh...
7 Pages(1750 words)Coursework

The Effect of Truth on the Protagonist Survival

Solzhenitsyn, a Nobel Prize winner, had been a rather obscure and unpublished 43-year-old teacher in a high school, situated in a Russian town, Ryazan, but suddenly in 1962, burst into the literary scene with his groundbreaking novel “One Day in the Life of Ivan Denisovich” which made him an overnight literary sensation. Besides being both compelling as well as authoritative, the truth of Stalin’s barbaric camps forms the mainstay of this novel. Solzhenitsyn’s simple but yet blatantly truthful messages about the prevalent Russian politics are elegantly woven into the story that erupted a plethora of protest from all quarters of society. Many of Solzhenitsyn stories were written in the labor and prison...
7 Pages(1750 words)Case Study

Effect of Corporations Moving Jobs Overseas on the Economy

The issue comes into focus, whenever there are political, economic debates or talks about H1B visa limits. The economic slowdown has further led to the intensification of these debates. But, despite apprehensions from different quarters, neither the companies nor the government has said a final word on the trend. Increasing levels of competition amongst the corporate houses have been one of the key highlights of the globalization era. Be it the retail sector, the banking, the automobile, the IT-enabled services, the white goods space, etc. the companies have always been on the lookout for reaching out to more number of customers while doing everything possible to please the existing lot. Leveraging the economies of scale has prove...
8 Pages(2000 words)Coursework

Entrepreneurship & Enterprise Activity

Onе of thе kеy problеms in еntrеprеnеurship rеsеаrсh rеlаtеs to thе lасk of сonsеnsus rеgаrding а dеfinition for еntrеprеnеurship. In fасt, mаny аuthors, suсh аs Spinosа (1997), Knight (2000), аnd Hjorth (2004) hаvе еmphаsisеd thе lасk of аn аgrееd dеfinition of еntrеprеnеurship аnd whаt еntrеprеnеurship сonstitutеs аs а fiеld of rеsеаrсh. Thе pаpеr disсussеs thе stаtеmеnt by Bridgеs (2003: p51) stаting thаt “Еntеrprisе асtivity is dеpеndеnt on еntеrprising individuаls” аnd аnаlysеs thе kеy thеoriеs thаt might hаvе rеlеvаnсе with this stаtеmеnt аnd to еvаluаtе whаt mаkеs onе pеrson morе еntеrprising thаn othеr.

Within thе fiеld of еntrеprеnеurship studiеs, it is dеbаtеd whеthеr thе сrеаtion of nеw сombinаtio...
7 Pages(1750 words)Case Study

Business Finance: Activity Based Costing

At that time, the western manufacturing companies were facing serious competition from their Japanese counterparts. The Japanese automotive and electrical products were capturing the market by their low costs. That prompted the Western companies to innovate new costing techniques that would point out their problems and lower the costs. This reason was the main catalyst in the acceptance of ABC. There was resistance by the management of companies who viewed ABC as detrimental to their interests. In addition, the cost of the impletion of ABC was huge which further disinterested the companies. But what the industry was going through in terms of innovation and competition was enough to pave the way for ABC’s smooth transformatio...
7 Pages(1750 words)Case Study

Economy and Social Activity of The Broken Hill Community

Soon a retail industry was built up and the community began to grow and is considered to be a society with a civic nature (Learning Communities in Education and Training, 2009). The community of Broken Hill can be considered to be highly diverse with a census that was conducted in the area in 1996 showed that out of the then population there were 961 people who came from overseas and a large amount of those was not from an English speaking nation. The large composition of the population in the community at the time was aboriginals or Torres Strait Islanders. Broken Hill thus shows a varying ethnic community and a diverse mindset considering a large proportion of the people are from nations such as Greece, Malta and even Italy (Lea...
6 Pages(1500 words)Case Study

The Effect of Incubation Temperature on Incubation

...The Effect of Incubation Temperature on Incubation Experimental Question Cellular respiration refers to a set of metabolic reactions and processes which occurs in the cells of plants and animals leading to the conversion of biochemical energy in the nutrients into adenosine triphosphate (ATP). It is also accompanied by the release of waste products. According to Rich, the reactions which take place in cellular respiration are catabolic and they lead to the breaking of large molecule into small molecules with energy being released (1089). Respiration is one of the key ways though which cells get energy used in fuelling of cellular activities. Cellular respiration is also considered to be an exothermic redox reaction due to the heat...
10 Pages(2500 words)Research Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic The Effect of Temperature on Enzymatic Activity of Amylase for FREE!

Contact Us