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Lab biochemistry - Coursework Example

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There are four subunits within NAD which consist of Zn2+ ions, the purpose of which is to aid in transfereing a hydride ion from the alcohol to NAD+ .
The graphs depicted above have…
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Lab biochemistry
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Download file to see previous pages The final set of graphs that needed to be plotted were the Eadie Hofstee plots. These are plots of V/[S] against velocity. All graphs for each alcohol had a negative gradient because of the inverse relationship between the V/S and velocity.
Only the first 4 plots were used in making the Absorbance vs. Time because using all the absorbance values would give a curvy orientation to the graph. The reason for this is that the enzyme sites were occupied by the substrates because of which there were no more enzymes sites where the substrate could bind. This is an indication of the Vmax meaning the all the catalytic sites have been occupied and the enzyme has become saturated with the ...Download file to see next pagesRead More
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