Proteins - Lab Report Example

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It is worth noting that there is no single most significant protein assay method known to cause utterly perfect results. This, thus, means that it all depends on the desired…
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Download file to see previous pages However, this method is somewhat expensive and a time consuming. There are various methods of protein quantification such as the Ultra violet Absorbance, Lowry Assay and BCA assay (Mathews, 2000). This experiment employed the Bradford protein assay method in determining of protein. The experiments entail a spectroscopic, analytical procedure used in measuring the concentration of proteins in any solution. The procedure in this method relies on the binding of the dye Coomassie BrilliantBlue G-250 to protein in the sample, in which the dye is proportional to the protein concentration. The bradford dye-binding assay is a colorimetric assay method for measuring protein concentration in solution samples. Much of it involves the binding of Coomassie Brilliant blue to protein. In this case, there is no interference from carbohydrates such as sucrose or from cat ions. However, some of the detergents like sodium dodecyl sulfate, as well as triton x-100 are capable of interfering with the assay, and the alkaline solutions. In this particular experiment, blank alongside six other test tubes were provided with a certain volume of Bovine albumin standard and the volume of some distilled water. The 1.5 mL of the unknown protein was constituted in the fourth test tube. The absorbance was then found through measuring it with the use of the Ultraviolet-visible spectrophotometer. The protein concentration was the computed with the help of the dilution equation. The determination of the unknown protein concentration was achieved through the use of two methods. These were linear regression method and the graphical methods.
Quantitative determinations of proteins is the most accurate yet more rapid method for estimating of protein concentration in the field of protein study. The Quantitative determinations of proteins can be titrimetric-elemental, gravimetric or spectroscopic (Stoscheck, 1990). An assay originally described by Bradford has ...Download file to see next pagesRead More
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