quora

Proteins - Lab Report Example

Comments (0) Cite this document
Summary
It is worth noting that there is no single most significant protein assay method known to cause utterly perfect results. This, thus, means that it all depends on the desired…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER97.8% of users find it useful
Proteins
Read TextPreview

Extract of sample
"Proteins"

Download file to see previous pages However, this method is somewhat expensive and a time consuming. There are various methods of protein quantification such as the Ultra violet Absorbance, Lowry Assay and BCA assay (Mathews, 2000). This experiment employed the Bradford protein assay method in determining of protein. The experiments entail a spectroscopic, analytical procedure used in measuring the concentration of proteins in any solution. The procedure in this method relies on the binding of the dye Coomassie BrilliantBlue G-250 to protein in the sample, in which the dye is proportional to the protein concentration. The bradford dye-binding assay is a colorimetric assay method for measuring protein concentration in solution samples. Much of it involves the binding of Coomassie Brilliant blue to protein. In this case, there is no interference from carbohydrates such as sucrose or from cat ions. However, some of the detergents like sodium dodecyl sulfate, as well as triton x-100 are capable of interfering with the assay, and the alkaline solutions. In this particular experiment, blank alongside six other test tubes were provided with a certain volume of Bovine albumin standard and the volume of some distilled water. The 1.5 mL of the unknown protein was constituted in the fourth test tube. The absorbance was then found through measuring it with the use of the Ultraviolet-visible spectrophotometer. The protein concentration was the computed with the help of the dilution equation. The determination of the unknown protein concentration was achieved through the use of two methods. These were linear regression method and the graphical methods.
Quantitative determinations of proteins is the most accurate yet more rapid method for estimating of protein concentration in the field of protein study. The Quantitative determinations of proteins can be titrimetric-elemental, gravimetric or spectroscopic (Stoscheck, 1990). An assay originally described by Bradford has ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Proteins Lab Report Example | Topics and Well Written Essays - 1250 words - 1”, n.d.)
Proteins Lab Report Example | Topics and Well Written Essays - 1250 words - 1. Retrieved from https://studentshare.org/biology/1605946-proteins
(Proteins Lab Report Example | Topics and Well Written Essays - 1250 Words - 1)
Proteins Lab Report Example | Topics and Well Written Essays - 1250 Words - 1. https://studentshare.org/biology/1605946-proteins.
“Proteins Lab Report Example | Topics and Well Written Essays - 1250 Words - 1”, n.d. https://studentshare.org/biology/1605946-proteins.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Proteins
There are many ways in which protein concentrations in substances, particularly solutions, can be determined. The most common approach is the application of a regression analysis model to develop a relationship between protein concentration in a solution and absorbance rate that can be experimentally determined.
5 Pages(1250 words)Lab Report
Molecular Basis of Pernicious Anaemia
Others include infiltration of inflammatory cells into the stomach and production of auto- antibodies to various proteins. It also involves the intrinsic factor and proton pump of parietal cells whereby intrinsic factor can be identified as the factor that is involved in the absorption of vitamin B12.
6 Pages(1500 words)Lab Report
DRVVT test
The aPTT is usually the duration it takes from when calcium is added to the formation of a clot. The nature of antibody is an Antiphospholipid antibody. The absence of positive antibodies in sample C means that lupus anticoagulants were not present in
2 Pages(500 words)Lab Report
HIV-1 Detection by Western Blot
The reaction is done when the viral protein are transferred to the nitrocellulose paper. The detection of the HIV virus through the process is detected through the antihuman
6 Pages(1500 words)Lab Report
Regulatory Genes
For the first experiment, the plasmids containing the mutant and wild type LHX3 and LHX3b isoforms genes were constructed and then cultured with human embryonic kidney 293T cells. The controls were set up by culturing the cells with empty DNA vector plasmids. The plasmids were taken up by the cells to express the genes encoded by these plasmids.
4 Pages(1000 words)Lab Report
The scientific method
Experiment 2 found that Peeps do not dissolve when sulfuric acid is injected directly into their bodies with a 30cc syringe, either. However, Experiment 3 proved that Peeps did dissolve almost entirely in Phenol, except for the eyes, which
1 Pages(250 words)Lab Report
Bioinformatics
Down syndrome can be diagnosed at birth: the doctor can look at the appearance of the baby or listen to the baby’s chest using a stethoscope. A blood test can also be done to confirm the diagnosis by checking the extra chromosome. I chose a
1 Pages(250 words)Lab Report
Molecular Fluorescence Analysis of Proteins
Fluorescence occurs when an excited molecule goes back to the ground state by photon emanation. Conversely, phosphorescence occurs when the excited electron
4 Pages(1000 words)Lab Report
Testing of biomolecules

The conclusion from this study states that establishing the presence of various biomolecules serves as the bedrock of successive analyses which include the way they work in the body. It is therefore important to understand the preliminary tests before understanding the various steps that follow.

5 Pages(1250 words)Lab Report
Protein Extraction and Gel Electrophoresis
Proteomic is generally defined as the direct analysis of proteins in terms of their presence and relative abundance. Gel electrophoresis is a significant methodology employed for extraction of proteins in proteome analysis. The most commonly used technique in gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
5 Pages(1250 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Proteins for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us