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However, this method is somewhat expensive and a time consuming. There are various methods of protein quantification such as the Ultra violet Absorbance, Lowry Assay and BCA assay (Mathews, 2000). This experiment employed the Bradford protein assay method in determining of protein. The experiments entail a spectroscopic, analytical procedure used in measuring the concentration of proteins in any solution. The procedure in this method relies on the binding of the dye Coomassie BrilliantBlue G-250 to protein in the sample, in which the dye is proportional to the protein concentration. The bradford dye-binding assay is a colorimetric assay method for measuring protein concentration in solution samples. Much of it involves the binding of Coomassie Brilliant blue to protein. In this case, there is no interference from carbohydrates such as sucrose or from cat ions. However, some of the detergents like sodium dodecyl sulfate, as well as triton x-100 are capable of interfering with the assay, and the alkaline solutions. In this particular experiment, blank alongside six other test tubes were provided with a certain volume of Bovine albumin standard and the volume of some distilled water. The 1.5 mL of the unknown protein was constituted in the fourth test tube. The absorbance was then found through measuring it with the use of the Ultraviolet-visible spectrophotometer. The protein concentration was the computed with the help of the dilution equation. The determination of the unknown protein concentration was achieved through the use of two methods. These were linear regression method and the graphical methods.
Quantitative determinations of proteins is the most accurate yet more rapid method for estimating of protein concentration in the field of protein study. The Quantitative determinations of proteins can be titrimetric-elemental, gravimetric or spectroscopic (Stoscheck, 1990). An assay originally described by Bradford has
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There are many ways in which protein concentrations in substances, particularly solutions, can be determined. The most common approach is the application of a regression analysis model to develop a relationship between protein concentration in a solution and absorbance rate that can be experimentally determined.
Others include infiltration of inflammatory cells into the stomach and production of auto- antibodies to various proteins. It also involves the intrinsic factor and proton pump of parietal cells whereby intrinsic factor can be identified as the factor that is involved in the absorption of vitamin B12.
The aPTT is usually the duration it takes from when calcium is added to the formation of a clot. The nature of antibody is an Antiphospholipid antibody.
The absence of positive antibodies in sample C means that lupus anticoagulants were not present in
For the first experiment, the plasmids containing the mutant and wild type LHX3 and LHX3b isoforms genes were constructed and then cultured with human embryonic kidney 293T cells. The controls were set up by culturing the cells with empty DNA vector plasmids. The plasmids were taken up by the cells to express the genes encoded by these plasmids.
Experiment 2 found that Peeps do not dissolve when sulfuric acid is injected directly into their bodies with a 30cc syringe, either. However, Experiment 3 proved that Peeps did dissolve almost entirely in Phenol, except for the eyes, which
Down syndrome can be diagnosed at birth: the doctor can look at the appearance of the baby or listen to the baby’s chest using a stethoscope. A blood test can also be done to confirm the diagnosis by checking the extra chromosome.
I chose a
The conclusion from this study states that establishing the presence of various biomolecules serves as the bedrock of successive analyses which include the way they work in the body. It is therefore important to understand the preliminary tests before understanding the various steps that follow.
Proteomic is generally defined as the direct analysis of proteins in terms of their presence and relative abundance. Gel electrophoresis is a significant methodology employed for extraction of proteins in proteome analysis. The most commonly used technique in gel electrophoresis is Sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
5 Pages(1250 words)Lab Report
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