quora
Nobody downloaded yet

Studies on Photosynthesis Using the Hill Reaction - Lab Report Example

Comments (0) Cite this document
Summary
This cellular process known as photosynthesis is carried out within the chloroplasts of these organisms and involves a series of sequential…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER98.2% of users find it useful
Studies on Photosynthesis Using the Hill Reaction Report
Read TextPreview

Extract of sample
"Studies on Photosynthesis Using the Hill Reaction"

Download file to see previous pages The primary ‘light reaction’ stage requires light and involves the excitation of electrons of the chlorophyll. These electrons then proceed along a series of electron carriers embedded in the thylakoid of the chloroplast resulting in synthesis of ATP in the stroma and the reduction of NADP. The dark reactions of photosynthesis do not directly require light involve the use of ATP and NADPH formed during the light reaction (figure 1) (Berg et al., 553).
Hill reaction named after its discoverer Robert Hill (1937) involves the photoreduction of an electron acceptor by the hydrogen atoms of water, along with evolution of oxygen. In laboratory, Hill reaction is demonstrated when isolated and illuminated ‘chloroplasts’ reduce an electron acceptor present with it, and release molecular oxygen:
The electron acceptors used in laboratory conditions are not the natural electron acceptors or NADP but instead are artificial electron acceptors (Walker, 109). These artificial electron acceptors intercept the electrons before they reach PS1700. Further they are generally dyes that change color when reduced e.g. DCIP (2,6-dichlorophenolindophenol). Thus Hill reaction can be written as:
Thus using the above equation, rate of Hill reaction in isolated protoplasts can be experimentally estimated. The change in absorbance of the dye at 600nm measured under varied conditions of experiment gives the rate of Hill reaction occurring under the preselected set of conditions. Several of the detergents are also known to be inhibitors of photosynthesis such as DCMU [3-(3, 4-dichlorophenyl)-1, 1-dimethylurea], DNP [dinitrophenol] and triton-X etc.
Chloroplast was extracted from deveined, washed and blot dried fresh baby spinach leaves. The standard fractionation protocol for extraction of chloroplasts was followed. 50g spinach leaves were homogenized in a pre-chilled blender jar along with buffered isotonic salt solution and antioxidant solution. The homogenate was ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Studies on Photosynthesis Using the Hill Reaction Report Lab”, n.d.)
Studies on Photosynthesis Using the Hill Reaction Report Lab. Retrieved from https://studentshare.org/biology/1582153-studies-on-photosynthesis-using-the-hill-reaction-report
(Studies on Photosynthesis Using the Hill Reaction Report Lab)
Studies on Photosynthesis Using the Hill Reaction Report Lab. https://studentshare.org/biology/1582153-studies-on-photosynthesis-using-the-hill-reaction-report.
“Studies on Photosynthesis Using the Hill Reaction Report Lab”, n.d. https://studentshare.org/biology/1582153-studies-on-photosynthesis-using-the-hill-reaction-report.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Report on photosynthesis lab
Our results indicate that apparent photosynthetic rate increases with temperature. We discuss the possible reasons for the observed pattern and discuss the implications that the relation may have for plant growth and productivity. Among several environmental variables, temperature has been known to strongly influence photosynthetic rate of plants (Blackman, 1905).
8 Pages(2000 words)Lab Report
Religious studies reaction paper
The arabesque is a motif of repeating geometric shapes and patterns. It is used mainly to avoid the engraving of worldly images - a taboo among Muslims. These patterns are seen in places like the Mosque of Cordoba. Although Islamic architecture applies these techniques in the finishing of buildings, the skyline of these structures is marked by the dome and minarets.
2 Pages(500 words)Essay
The Impact of Light Wavelength to Photosynthesis
With the assumption that the rate of photosynthesis as manifested by CO2 consumption would be inversely related to the received light wavelength, drops in CO2 recorded and data calculated. Green wavelength having the lowest rate of photosynthesis was the only data adhering to what we had predicted.
6 Pages(1500 words)Lab Report
Photosynthesis
The rate of photosynthesis is affected by factors like: carbon dioxide concentration, temperature and light intensity. If two of these factors are kept constant while one of
2 Pages(500 words)Lab Report
Photosynthesis
Complex reaction of photosynthesis that takes place in the leaves can be summarized as follows: An experiment was carried out to investigate the effects of color of
4 Pages(1000 words)Lab Report
Complete a lab report using the scientific method
Prior research has reported that the influence of soil variability and the crop performance is extremely pronounces in regions of the tropical (Moorman, 2005). In
1 Pages(250 words)Lab Report
Photosynthesis
oxygen and carbon (IV) oxide (Berg 71). In this experiment, Light as a necessity of photosynthesis is the variable under investigation. According to the research design in this experiment, leaf disks float
2 Pages(500 words)Lab Report
Lab report (Using NMR)
The chemical shift targeting each nucleus can be correlated to the chemical environment with the scalar coupling showing an indirect interaction between the individual nuclear that are mediated by the electrons found in the chemical bond and appropriate
4 Pages(1000 words)Lab Report
Does the Changing the color of light will affect the rate of photosynthesis
The control variable used was white light on the plant while green and blue light were the dependent variables. White light contains seven colors inclusive of blue and green, hence their dependency. White light was expected to
1 Pages(250 words)Lab Report
Identification of GMO foods using PCR Lab Report
The genetically modified reference standards were used as controls and the samples were analyzed using agarose gel electrophoresis. GMOs was not identified in all of our soy beans samples with PCR method, we rejected our null hypothesis that stated GMOs was present in our soy beans samples.
3 Pages(750 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Studies on Photosynthesis Using the Hill Reaction Report for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us