The paper "Catabolite Repression and Induction of Beta-galactosidase Synthesis" demonstrates both catabolite repression and induction of beta-galactosidase synthesis during growth of E. coli Synthesis of the enzyme beta-galactosidase is induced in wild-type E. coli strains in response to the presence of lactose…
Download full paperFile format: .doc, available for editing
Extract of sample "Catabolite Repression and Induction of Beta-galactosidase Synthesis"
Download file to see previous pages
Apart from induction, synthesis rate is determined by catabolite repression, whereby it slows down the synthesis of beta-galactosidase especially in the presence of a better carbon (and energy) source, such as glucose. In this experiment, Escherichia coli (E. coli) is used as the bacteria to induce synthesis of enzyme β-galactosidase. The results support lactose metabolism by newly synthesised beta-galactosidase and also, quantitatively, IPTG is a more effective inducer of beta-galactosidase synthesis than lactose.
In order to regulate the gene expression levels in a cell, there are certain mechanisms that must be considered in operation. In which case, the regulation is considered at transcription and translation levels or the stability of messenger RNA. The aforementioned can only work in regulation based on the synthesis of a particular protein. Consequently, it comes out as a subject of importance to investigate the regulation of transcription of bacterial genes. For this case, Escherichia coli (E. coli) is used as the bacteria to induce synthesis of enzyme β-galactosidase.
Escherichia coli (E. coli) can produce the enzyme β-galactosidase which breaks lactose into galactose and glucose. Synthesis of the enzyme beta-galactosidase is induced in wild-type E. coli strains in response to the presence of lactose, the enzymes natural substrate (Ring, 1999, 80). The inducer, lactose, is usually the molecule broken down by the enzyme system. Worth noting is the ability of E.coli to solely use lactose as a carbon source regardless of the presence of glucose. A more stable inducer that lactose, IPTG (Isopropyl β-D-1-thiogalactopyranoside) helps in inducing expression of the enzyme without being metabolized in the process.
Apart from induction, synthesis of β-galactosidase is also influenced by catabolite repression. The process involves slow down of the synthesis process, facilitated by lactose, especially when a presence of glucose is detected. Intuitively, glucose acts as a better energy and carbon source than lactose (Wallenfels, 1972, 67). When given both sugars, E.coli will not synthesize beta-galactosidase until all of the glucose is first exhausted from the medium.
...Download file to see next pagesRead More
Cite this document
(“Catabolite Repression and Induction of Beta-galactosidase Synthesis Lab Report”, n.d.)
Catabolite Repression and Induction of Beta-galactosidase Synthesis Lab Report. Retrieved from https://studentshare.org/biology/1685209-writers-choice
(Catabolite Repression and Induction of Beta-Galactosidase Synthesis Lab Report)
Catabolite Repression and Induction of Beta-Galactosidase Synthesis Lab Report. https://studentshare.org/biology/1685209-writers-choice.
“Catabolite Repression and Induction of Beta-Galactosidase Synthesis Lab Report”, n.d. https://studentshare.org/biology/1685209-writers-choice.
(Gropper P 333). Vitamin A can be derived from plant and animal sources. However, plant sources (cartenoids) must go through additional enzymatic activity in order to be converted to retinol. The focus of this paper will be to describe what was known of the conversion of -carotene to vitamin A in the 1920's and what is known today.
This research will begin with the statement that in electromagnetic induction, a current carrying wire induced a magnetic field around the wire. Cutting the field using a magnet will result to an induced emf, which is proportional to the rate of change of the magnetic field or speed at which the field is cut.
al product promotion is not something that started in recent times, many of the earliest entrepreneurs knew how to produce goods and make communicational strategies that would increased people’s awareness of their products to influence their purchase patterns.
In the United
Money swings in the market, when financial crises arises in company, how financial needs are managed viz., in stock market most probably finance inflation may rise or fall and at the same time company's share market also fluctuates that may effect bank interest rate.
On the other hand, Naomi Klein, who is a Canadian author as well as social activist known for her criticism for corporate globalization explores the evolution of branding and advertising in her article titled New Branded World. Further, New York Based
The ideas on the regulation of protein synthesis in bacteria have mainly arisen from the studies of Perse Monod on the induced synthesis of B-galactosidase in E.coli. It resulted in the discovery of regulatory events to the transcription of DNA and the assemblage of functionally related genes into clusters called operons.
Sue Holtand John Miller, and the MIT Open-source website (http://ocw.mit.edu/high-school/physics/demonstrations-on-video/conductors-capacitors-dielectrics/).
According to Advanced Physics for You by Keith Johnson, Simmone Hewett, Sue Holt and John Miller, the approach of
5 Pages(1250 words)Essay
GOT A TRICKY QUESTION? RECEIVE AN ANSWER FROM STUDENTS LIKE YOU!
Save Your Time for More Important Things
Let us write or edit the lab report on your topic
"Catabolite Repression and Induction of Beta-galactosidase Synthesis"
with a personal 20% discount.