Nobody downloaded yet

Catabolite Repression and Induction of Beta-galactosidase Synthesis - Lab Report Example

Comments (0) Cite this document
Summary
The paper "Catabolite Repression and Induction of Beta-galactosidase Synthesis" demonstrates both catabolite repression and induction of beta-galactosidase synthesis during growth of E. coli Synthesis of the enzyme beta-galactosidase is induced in wild-type E. coli strains in response to the presence of lactose…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER92.1% of users find it useful
Catabolite Repression and Induction of Beta-galactosidase Synthesis
Read TextPreview

Extract of sample
"Catabolite Repression and Induction of Beta-galactosidase Synthesis"

Download file to see previous pages Apart from induction, synthesis rate is determined by catabolite repression, whereby it slows down the synthesis of beta-galactosidase especially in the presence of a better carbon (and energy) source, such as glucose. In this experiment, Escherichia coli (E. coli) is used as the bacteria to induce synthesis of enzyme β-galactosidase. The results support lactose metabolism by newly synthesised beta-galactosidase and also, quantitatively, IPTG is a more effective inducer of beta-galactosidase synthesis than lactose.
In order to regulate the gene expression levels in a cell, there are certain mechanisms that must be considered in operation. In which case, the regulation is considered at transcription and translation levels or the stability of messenger RNA. The aforementioned can only work in regulation based on the synthesis of a particular protein. Consequently, it comes out as a subject of importance to investigate the regulation of transcription of bacterial genes. For this case, Escherichia coli (E. coli) is used as the bacteria to induce synthesis of enzyme β-galactosidase.
Escherichia coli (E. coli) can produce the enzyme β-galactosidase which breaks lactose into galactose and glucose. Synthesis of the enzyme beta-galactosidase is induced in wild-type E. coli strains in response to the presence of lactose, the enzymes natural substrate (Ring, 1999, 80). The inducer, lactose, is usually the molecule broken down by the enzyme system. Worth noting is the ability of E.coli to solely use lactose as a carbon source regardless of the presence of glucose. A more stable inducer that lactose, IPTG (Isopropyl β-D-1-thiogalactopyranoside) helps in inducing expression of the enzyme without being metabolized in the process.
Apart from induction, synthesis of β-galactosidase is also influenced by catabolite repression. The process involves slow down of the synthesis process, facilitated by lactose, especially when a presence of glucose is detected. Intuitively, glucose acts as a better energy and carbon source than lactose (Wallenfels, 1972, 67). When given both sugars, E.coli will not synthesize beta-galactosidase until all of the glucose is first exhausted from the medium. ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Catabolite Repression and Induction of Beta-galactosidase Synthesis Lab Report”, n.d.)
Catabolite Repression and Induction of Beta-galactosidase Synthesis Lab Report. Retrieved from https://studentshare.org/biology/1685209-writers-choice
(Catabolite Repression and Induction of Beta-Galactosidase Synthesis Lab Report)
Catabolite Repression and Induction of Beta-Galactosidase Synthesis Lab Report. https://studentshare.org/biology/1685209-writers-choice.
“Catabolite Repression and Induction of Beta-Galactosidase Synthesis Lab Report”, n.d. https://studentshare.org/biology/1685209-writers-choice.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Electromagnetic induction
...In electromagnetic induction, a current carrying wire induced a magnetic field around the wire. Cutting the field using a magnet will result to an induced emf, which is proportional to the rate of change of the magnetic field or speed at which the field is cut. This is the Faraday’s law being investigated in this experiment. on the other hand, the experiment portrays that the magnetic flux will always act as to oppose any change in a closed loop, which is the Lenz’s law investigated in this experiment. The results showed that the polarity of the induced voltage opposed the charge producing it, with the speed of the magnet cutting the field being propotional to the emf induced. Moreover, when testing the polarity of a...
9 Pages(2250 words)Assignment
Electromagnetic induction
...? Electromagnetic Induction Electromagnetic induction is a principle, which predicts the behaviour of the electric currentswhenever they interact with the magnetic field in the production of the electromotive force. It is a basic principle of operation for many types of electric devices such as motors. In order to understand the characteristics of induction, an experiment set out to investigate the operation of electromagnetic induction. The prime aim of this experiment was to investigate the magnets and coils behaviour, to test Lenz’s law and Faraday’s law, and to test the transformers behaviour. Introduction Electromagnetic induction involves the...
4 Pages(1000 words)Essay
Beta
...VALUE OF BETA Beta is defined as "a measure of a security's or portfolio's volatility, or systematic risk, in comparison to the market as a whole. Also known as beta coefficient" (Jesse). Beta is related how to manage money, it can be for personal budgeting or to run a company. Money swings in the market, when financial crises arises in company, how financial needs are managed viz., in stock market most probably finance inflation may rise or fall and at the same time company's share market also fluctuates that may effect bank interest rate. Company's (like Wal-Mart, Kmart, etc.,) security returns will also be affected. If oil price are increased in the market...
2 Pages(500 words)Essay
Set Induction
...Set Induction Set induction is the preparation that a teacher or makes for a formal lesson. The preparation is normally to help students to get ready to learn a new concept (Schuck, 227). In other words, set induction is about inducing students or learners into a mind-set that will help them develop interest for the new subject. In all set inductions, there are some sets that are usually used just before any new activity is introduced to students. An effective set should have a level of clarity so that students are aware of what to expect from a new activity. The set should be clear enough for the students to know what is expected of them. It should also help them have...
3 Pages(750 words)Essay
Beta golf
...Beta Group A. Beta Group Founding and Business Model Beta Group was started by Bob Zider after a long career with one of the major consulting firms. He saw a disconnect between technological development and the way that large companies spent their research and development budgets. They spent most of their funds to support and develop their existing product lines rather than innovating or applying new technology to available markets. He also saw a lack of funding for research and development in initial investment organizations like venture capital firms. The lack of funding from venture firms and the inefficient application of research capital led to the founding of Beta...
3 Pages(750 words)Assignment
Synthesis
... Attributes Of Fear in Achievement Research question: What damage can fear cause in one’s ability? A dispute can clearly be seen in the two texts on the impact of the word broken. According to Franklin, ‘broken’ can be used in two ways; first she talks about it being used positively, that is, a contestant was told by the judges that she looked good broken. This is a positive comment that used the word ‘broken’, as the judges praised the picture. It is also seen that the judges use ‘broken’ in a negative way, whereby, they say “You look like the broken Tin Man”. Franklin concludes, “sometimes looking broken is good; sometimes it’s not”. However, she dislikes the criticisms from the judges. On the other hand, Amy Tan dislikes... Attributes Of...
5 Pages(1250 words)Essay
Synthesis
...Synthesis Scenario Auditory James should use Socratic learning method, where he should ask his what they know about symbolism, metaphor and simile. James must draw as much information from them as possible, filling gaps with his own expertise. Visual Visual tools can enhance and aid James in teaching, as then he can invite the students to participate in the lecture, which would keep them alert to the auditory environment as well. Kinesthetic James can adapt Kinesthetic learning strategy by asking students to use similes, metaphors and symbols in their daily conversations. He can initiate it by forming two teams, dividing up the class, and initiating a competition among them. This would make students active participants,...
2 Pages(500 words)Essay
Forced induction
...Forced Induction Forced induction is a critical process in automobiles; it refers to the process whereby compressed air is delivered to the internal combustion engine intake. It is such an important process to an extent that an engine that does not have it is considered to be naturally aspirated (Nag 16). This process increases the density, temperature, and pressure of the air using a gas compressor. It is used in the aviation, as well as automobile industry in increasing the efficiency and power of the engine (Rathakrishnan 41). This paper will discuss the process of forced induction and its various aspects. It is worth noting that the gas compressors are two in series. The first one...
3 Pages(750 words)Research Paper
Synthesis
...CHARGING AND DISCHARGING CAPACITORS AND CAPACITANCE + Submitted Charging and Discharging Capacitorsand Capacitance Capacitance is an aspect of Physics that is essential in our daily lives. The essay intends to exemplify the critique of two websites in terms of the way they each approach capacitance and capacitors. The two sources,are Advanced Physics for You by Keith Johnson, Simmone Hewett, Sue Holtand John Miller, and the MIT Open-source website (http://ocw.mit.edu/high-school/physics/demonstrations-on-video/conductors-capacitors-dielectrics/). According to Advanced Physics for You by Keith Johnson, Simmone Hewett, Sue Holt and John Miller, the approach of capacitance is from a simplified version. It states that a capacitor... AND...
5 Pages(1250 words)Essay
B-Galactosidase Enzyme Activities
...from the studies of Perse Monod on the induced synthesis of B-galactosidase in E.coli. It resulted in the discovery of regulatory events to the transcription of DNA and the assemblage of functionally related genes into clusters called operons. The specific mechanisms exist to regulate the levels of expression in the cell. The regulation may be as a result of transcription, translation or messenger RNA stability. In this experiment, there is the regulation of transcription of bacterial genes that are either inducible or repressible enzyme systems. In induced enzymes, there is the breakdown of complex molecules for example lactose induces synthesis of the proteins...
6 Pages(1500 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Catabolite Repression and Induction of Beta-galactosidase Synthesis for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us