Interferences in the Assay of Serum Albumin Using the Bromcresol Green Indicator - Lab Report Example

Comments (0) Cite this document
Summary
Performing a simple experiment using the Bromcresol-Green(BCG) method for Albumin concentration determination in human serum an observation was made that upon the addition of protein globulins an interference occurs raising the result significantly. This finding concurs with a literature review that was performed…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.9% of users find it useful
Interferences in the Assay of Serum Albumin Using the Bromcresol Green Indicator
Read TextPreview

Extract of sample
"Interferences in the Assay of Serum Albumin Using the Bromcresol Green Indicator"

Download file to see previous pages Albumin's molecular structure was reported from research in the past 30 years. Albumin's clinical importance was discussed, explaining that the test for Albumin is not a marker for any specific disease, while it is an important indicator for the physiological condition of the body. It was explained that an abnormally high result for Albumin needs to resolved and at face-value is considered a spurious result. The cause of elevated albumin levels are usually a result of accompanying serum proteins, acute phase infections, elevated immunoglobulins(found in multiple myeloma), or autoimmune issues. The BCG method was thoroughly researched. It is a valuable test for screening, when performing mass analysis of blood work in large clinical laboratories. It has fallen out of favor with laboratory clinicians for fear that the test can mask hypoalbunemia and low readings. BCG is an acid-base indicator, its acid to base color change is from yellow to blue, with a range in pH from 4.2-5.6. The detection of hypoalbunemia is always important to a physician. Low Albumin readings are indicative of a deteriorating condition in the body. An alternative to the BCG indicator is Bromcresol Purple(BCP), also an acid–base indicator. A comparison studies have been performed of the two methods. When compared to a benchmark analytic method Capillary Zone Electrophoresis(CZE), the positive bias for BCG was 3.77, while BCP faired much better with 0.67. Our experiment produced linear curves needed in detection and quantification of albumin concentrations. We demonstrated the effect on accompanying globulins elevating the result in BCG determinations of albumin. We also showed that time, reagent concentration and wavelength of absorbance significantly affect albumin readings. We bring to consideration for further testing the affect of pH on the test.


















Background and Literature review
In clinical medical laboratories the testing for serum albumin, globulins and total protein are standard procedures, performed daily. Theses assays may also be requested STAT or on an emergency basis for patients suffering a crisis situation. The techniques for determining albumin and total protein are numerous and varied. Most clinical laboratories perform three routine three routine examinations;
1) Total Protein(TP)
2) Serum Albumin(Alb)
3) Serum Globulins
Human blood contains both a cellular and liquid portion. Albumin is the most abundant protein in the human circulatory system1 and contributes 80% to providing colloid osmotic pressure2. Albumin is chiefly responsible for maintenance of the blood's acid-base balance, commonly referred to as pH3. In mammals it is synthesized by the liver as a preproalbumin. It undergoes a two cleavage processes before release into the body's circulation in its final form. It has been determined that the half-life of a albumin molecule is 19 days4..

Figure 1: Molecular Cleavage of albumin from preproalbumin->proalbumin->albumin

Figure 2: The classical perception of the Albumin molecule

Peters, T., Jr. (1985). Serum Albumin. Adv. Protein Chem.37; 161-2455
Figure 3:
Primary, secondary and tertiary structure

(Carter and Ho, 1994) . 6
This picture shows the bovine albumin amino acid sequence. The BSA molecule is made up of three homologous domains (I, II, III) which are divided into nine loops (L1-L9) by 17 disulphide bonds. The loops in each domain are made up of a sequence of large-small-large loops forming a triplet. Each domain in turn is the product of two subdomains (IA, IB, etc.). The primary structure of albumin is unusual among extracellular proteins in possessing a single ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Interferences in the Assay of Serum Albumin Using the Bromcresol Green Lab Report”, n.d.)
Retrieved from https://studentshare.org/science/1528526-interferences-in-the-assay-of-serum-albumin-using-the-bromcresol-green-indicator
(Interferences in the Assay of Serum Albumin Using the Bromcresol Green Lab Report)
https://studentshare.org/science/1528526-interferences-in-the-assay-of-serum-albumin-using-the-bromcresol-green-indicator.
“Interferences in the Assay of Serum Albumin Using the Bromcresol Green Lab Report”, n.d. https://studentshare.org/science/1528526-interferences-in-the-assay-of-serum-albumin-using-the-bromcresol-green-indicator.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
DETECTION OF ANTIVIRAL ANTIBODIES IN SERUM USING AN ELISA TECHNIQUE
Antibodies are the fundamental reagents in ELISA. The determination of the presence or absence of the antibody concentration in the blood enables us to identify the disease and understand the level of the disease. Antibodies form a type of glycoproteins in the serum of the blood and they are called as immunoglobulins.
5 Pages(1250 words)Essay
Effects of Green and Blue Light on the Rate of Photosynthesis
Photosynthesis is the process through which green plants, algae, and cyanobacteria convert the sun’s energy into chemical bond energy of organic molecules that it consumes as food. This paper covers two experiments on photosynthesis; the first one investigates whether a positive rate of net photosynthesis depends on light levels.
5 Pages(1250 words)Lab Report
The Green Oxidation of Cyclohexanol to Cyclohexanone
In the second step, cyclohexanone was isolated from the cyclohexanone-water mixture in a salting out procedure using sodium chloride and ether. The extracted cyclohexanone had a refractive index (n20/D) of 1.467, which is close to the literature value of n20/D 1.450, indicating that the isolated cyclohexanone was of good purity.
6 Pages(1500 words)Lab Report
Electrophoretic mobility shift assay
The SP members are essential for metastasis and regulate the expression of more than one gene in cells. Experiment was conducted to perform the EMSA and used P21 promoter that contained 6 sp sites. SP1 was used as an antibody. Sample of nuclear extract was nuclear lystae produced from the colorectal cancer cells with multiple nuclear proteins.
8 Pages(2000 words)Lab Report
Determination of Protein using Biuret Reagent
In case the bond is detected, copper (II) ions turn violet in an alkaline solution. In performing this test, several variants can be considered. Despite the name, the chemical does not contain burette ((H2N-CO-)2NH). This reaction is named using that name because it
3 Pages(750 words)Lab Report
ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) OF PLASMA IgM
In this experiment we have conducted the solid-phase sandwich ELISA which allows for detection and quantification of the antigen (Stryer et al, 2002). Here, the antibody is immobilized in wells and antigens are allowed to react with it. After
3 Pages(750 words)Lab Report
Horseradish peroxidase assay
They only change the rate of reaction (Kovarik & Allbritton, 2011). The structures of enzymes comprise of a globular protein and a cofactor. The bonds holding the proteins in their structures are affected by temperature and pH changes. Such changes affect the
6 Pages(1500 words)Lab Report
Flow Measurement Using Venturi Meter
This means that it encompasses the actual experimental set-up, the used apparatus, the achieved results, the theoretical framework of this concept, the analysis and discussion of the attained
9 Pages(2250 words)Lab Report
Lab report (Using NMR)
The chemical shift targeting each nucleus can be correlated to the chemical environment with the scalar coupling showing an indirect interaction between the individual nuclear that are mediated by the electrons found in the chemical bond and appropriate
4 Pages(1000 words)Lab Report
Displacement Using LVDT
LVTD (Linear Variable Differential Transformer) is a position sensitive device that is mainly used in the provision of AC output which is proportional to the displacement that occurs in its core passing through its wings. It works in the principle of mutual induction. It consist three systematically spaced coil bound out and illustrated bobbin.
3 Pages(750 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Interferences in the Assay of Serum Albumin Using the Bromcresol Green Indicator for FREE!
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us