CHECK THESE SAMPLES OF Determination of Proteins Using Biuret and Lowry Assay Techniques
In the paper “Determination of Protein using biuret Reagent,” the author describes protein as an indispensable or principal class of cellular macromolecules.... The blue color appears as a result of the presence of proteins, pink and blue may also arise in the presence of short-chained polypeptides.... However, the analysis show assay below 0.... In many experiments, biuret can be scaled using smaller sized cuvette, which consumes less protein....
3 Pages
(750 words)
Assignment
There are various methods of protein quantification such as the Ultra violet Absorbance, lowry assay and BCA assay (Mathews, 2000).... The experiments entail a spectroscopic, analytical procedure used in measuring the concentration of proteins in any solution.... Quantitative determinations of proteins is the most accurate yet more rapid method for estimating of protein concentration in the field of protein study.... The Quantitative determinations of proteins can be titrimetric-elemental, gravimetric or spectroscopic (Stoscheck, 1990)....
9 Pages
(2250 words)
Lab Report
he spectrophotometer zero is set to using one of the reagent blanks (reaction set up with water).... The spectrophotometer zero is set to using one of the reagent blanks (reaction set up with... To each labeled microcentrifuge tube 50 μl of each different protein standard (D,E,F,G and H) or water (for reagent blanks) or unknown samples X, Y and Z are added....
5 Pages
(1250 words)
Lab Report
There has been many methodologies produced for protein analysis that proves to be accurate and produce good results which include the Bradford Assay and lowry assay considered as the most common method (Knight and Chambers, 2003), Biuret Assay, Bichinchoninic Acid Assay.... Other modern techniques are also developed such as DNA Pull down Assay, Microplate Capture and DetectionAssay, Electrophoretic Mobility Shift Assay, and more (Pierce Protein Biology Products, 2014)....
4 Pages
(1000 words)
Essay
Thus, the biuret protein assay test is a concrete test that can be used in the determination of the peptide bonds and the amount of proteins in a solution (Lipscomb et al.... The Biuret test in this experiment is used in the determination of the concentration since peptide bonds occur with the same frequency in each amino acid in the peptide.... Folin protein assay is a biochemical assay for determination of the total level of protein in a solution....
5 Pages
(1250 words)
Lab Report
Our muscles are made of proteins, and we derive our ability to actuate by our forces only.... Therefore, it is not surprising that the concentration of proteins is one of the essential biochemical parameters while studying biochemistry or biomedical sciences.... Spectrophotometry is a technique widely used for concentration determination of many other species in chemistry as well.... Three different colorimetric methods used for the determination of protein assays will be used in this experiment to decide the assays of mitochondrial protein....
7 Pages
(1750 words)
Lab Report
hellip; In the case of proteins, a solution is allowed to react with the Biuret reagent (a combination of a base such as potassium hydroxide and copper II sulfate); the copper forms a complex with the peptide bonds of the protein and the resulting solution has a strong purple color, with maximum light absorption at a wavelength of 540 nm.... The concentration of such proteins in the bloodstream is an important biomarker for these and other medical problems....
7 Pages
(1750 words)
Lab Report
This can be done by the 'modified lowry method': a Biuret reagent consisting of a strong base and a source of copper ions such as copper II sulfate is combined with the sample to be tested.... hellip; One of the best ways of doing so is to expose a sample of protein in solution to the biuret reagent, which combines with a protein's peptide bonds to form a complex with a pronounced purple color, and then use a spectrophotometer to measure the resulting solution's absorption of light of 540-nm wavelength....
8 Pages
(2000 words)
Lab Report