CHECK THESE SAMPLES OF Estimation of Protein Concentration by Spectrophotometry
The study of protein concentration in the body, as well as in substances is therefore fundamental to nutritionists and health care professionals.... This paper applies the Bradford's method to investigate the relationship between protein concentration and absorbance rate.... The study is based on established assumption that observed rate of absorption is proportional to protein concentration and developed relationship between known concentrations and observed absorbance rates can be used to determine unknown concentrations, given their absorbance rates....
3 Pages
(750 words)
Lab Report
The movement of the molecules depends on the size and charge taken up by each type of protein (Kaneko et al, 2008).... Liver function can be appropriately evaluated by measuring the total protein and albumin in the plasma sample and if a low concentration is detected, that means liver functioning is compromised (Gerardo 1998).... Therefore, the total protein and albumin levels have been tested in this particular patient to evaluate his liver function....
6 Pages
(1500 words)
Essay
Type of medium and concentration of the gel determines the gel's pore size and its ability to segregate same sized fragments.... Purification of Genomic DNA from Buccal Cells Name Class Professor Course Date 1.... Purification of Genomic DNA from Buccal Cells 2.... Introduction 2....
9 Pages
(2250 words)
Essay
Bradford protein concentration Assay Name: Institution: Instructor: Course: Date: Abstract The measurement of protein concentrations in aqueous samples is an important assay in biochemistry research and development laboratories for applications such as enzymatic studies and also in providing data for biopharmaceutical lot release.... The following is a report on an experiment conducted to determine protein concentration of unknown samples using this method.... Bradford protein concentration Assay Accurate protein quantitation is paramount to all experiments that are related to proteins in a lot of research topics in molecular biology, developmental biology, cell biology, neuroscience, and biochemistry....
5 Pages
(1250 words)
Lab Report
To each labeled microcentrifuge tube 50 μl of each different protein standard (D,E,F,G and H) or water (for reagent blanks) or unknown samples X, Y and Z are added.... 1 ml of the BCA reagent is added,and mixed well by inverting the capped tubes.... he rack is transferred to a 60oc… After the 30 minutes of incubation the rank is removed from the water bath and the samples allowed to cool....
5 Pages
(1250 words)
Lab Report
The existence of a myriad of protein isolation and purification techniques is a direct consequence of these scientific studies (Jordan and Ogren,… Enzymology entails the use of isolation and purification techniques similar to those used in proteomics.... Ribulose 1, 5-bisphosphate carboxylase/oxygenase (rubsico) is one of protein Purification Protein Purification Introduction Majority of scientific studies today require the isolation, purification and study of micro-molecules in biochemical processes....
2 Pages
(500 words)
Lab Report
Is the process of converting all the phosphates in the form appropriate that reacts with the colorimetric reagent, and also to eliminate interferences which could be caused by other present species (Nutrient 2000) and (Water quality program 2006) g) Calculation of concentration.... In order to calculate the concentration of the analyte, a calibration curve should be constructed, based on the absorbance measurement of at least four standards of known concentration in the appropriate range....
8 Pages
(2000 words)
Term Paper
This work "Measuring Metabolites in Plasma Samples by Flame Photometry and spectrophotometry" focuses on the concentration of plasma urea and creatinine.... Reabsorption also makes the excreted urea an inaccurate reflector of GFR Urea measurement should not be used as a general test of renal function however in certain circumstances related to renal disease it is useful; suspected prerenal renal failure due to fluid depletion or cardiac failure, where plasma urea will tend to rise before plasma creatinine concentration....
6 Pages
(1500 words)
Research Paper