quora

Immunosorbent assay (ELISA) - Essay Example

Comments (0) Cite this document
Summary
The search for, determination and detection of antibodies and antigens in an organism’s body can be enhanced by using a technique known as the enzyme-linked immune-sorbent assay (ELISA). ELISA, as a laboratory procedure, determines the absorption of analyte (antibodies or…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER97.8% of users find it useful
Immunosorbent assay (ELISA)
Read TextPreview

Extract of sample
"Immunosorbent assay (ELISA)"

Download file to see previous pages A comprehensive understanding of how an ELISA works would require the determination of what antibodies are and how they work. The basic definition of an antibody is that it is a protein produced by the immune structure in reaction to the foreign molecules in an organism’s body.
Under circumstances that require the attainment of indirect semi-quantitative results, multiwall polystyrene wells are the most commonly used solid surface. ELISA, being quick and easy to undertake, is preferable given that its makeup also enables it to tackle voluminous samples consecutively during research and diagnostic targets. As a result, ELISA is considered the best choice when evaluating research and diagnostic targets (Killian et al., 2013). The process of performing ELISA depends on the relations between an epitope; it may be a small linear or a three-dimensional succession of amino acids established on an antigen and a corresponding antibody-binding site.
This experiment aimed at determining how the concentration of antibodies in the body would be affected by the use of ELISA under indirect semi-quantitative and anti-human albumin antibody. This is because of the wide utilization or applications to which the enzyme can be put such as in the analytical implements in biomedical research to detect and quantify specific antibodies and antigens in a sample. In this experiment, the antibodies employed were either monoclonal or polyclonal (Rosie, 2013). When performing any ELISA test, there are four formats of performing the laboratory tests: direct ELISA, indirect ELISA, sandwich/capture ELISA, and competitive or inhibitor ELISA. These allows the experiment’s elasticity to fit basing on antibodies available, the needed results and complications of the essential results (Rodland, 2005.p.145-148). In this experiment, the variation employed is the indirect ELISA, which employs the human albumin ELISA test.
Given that ELISA test utilizes the rudimental concept ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Immunosorbent assay (ELISA) Essay Example | Topics and Well Written Essays - 1750 words”, n.d.)
Retrieved from https://studentshare.org/biology/1652131-immunosorbent-assay-elisa
(Immunosorbent Assay (ELISA) Essay Example | Topics and Well Written Essays - 1750 Words)
https://studentshare.org/biology/1652131-immunosorbent-assay-elisa.
“Immunosorbent Assay (ELISA) Essay Example | Topics and Well Written Essays - 1750 Words”, n.d. https://studentshare.org/biology/1652131-immunosorbent-assay-elisa.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Explain the rationale behind antibody usage in common laboratory assays such as ELISA, immunofluorescence, immunohistochemistry,
?.10 Figure4: Principles of immunohistochemistry………………………………………………12 Figure 5: Steps involved in western blotting………………………………………………..14 The Rationale behind Antibody Usage in Common Laboratory Assays Such As ELISA, Immunofluorescence, Immunohistochemistry, and Western Blotting, their Relative Advantages and Disadvantages Introduction The immune system produces antibodies in reaction to an assortment of foreign substances as well as infections in the body.
10 Pages(2500 words)Essay
The Molecular Detection Methods For Plasmodium Falciparum
There are also other plasmodium species which cause malaria. These are plasmodium ovale, plasmodium malariae and plasmodium vivax which are considered as less dangerous species. Malaria is spread by the female anopheles mosquito which while feeding on human blood injects its saliva into the body.
10 Pages(2500 words)Essay
Applied Immunology Essay Question
The diluted serum suspected to be containing specific immunoglobulin antibody for bound antigen is added to the ELISA plate/ well and allowed time to react. Enzyme labeled immunoglobulin is then added, allowed time for
3 Pages(750 words)Essay
Detection of the Diurnal Rhythm of Cortisol Secretion
Cortisol is also released in reaction to the “fight or flight” response in human beings and must be regulated later for normal body functioning (Nelson, 2011).
4 Pages(1000 words)Essay
ELISA ASSAY
It is important to use multichannel pipette to avoid any manual error at a time of filling reagents to microtiter plates. Similarly, to avoid error like at concentration of 2.0 there must be at least 3 replicates which make data more meaning full and realistic in terms of statistic.
2 Pages(500 words)Essay
Immunology and Immune Disorders
The reason for which ELISA is favoured foe biological tests is that its reactions can be measured both qualitatively and quantitatively. Consequent to this advantage, ELISA is used in biological and medical researches and in clinical setting for tests and diagnoses (Icon Group International, 2011).
4 Pages(1000 words)Essay
Enzyme Linked Assays
5 Pages(1250 words)Essay
Chlamydia
common representative of Chlamydia genus is Chlamydia trachomatis which can cause Chlamydia infection – an infectious disease that is transmitted through sex. It is one of the most common sexually transmitted disease. Infection with chlamydia usually transfers through sex,
3 Pages(750 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic Immunosorbent assay (ELISA) for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us