quora
Nobody downloaded yet

Diurnal Rhythm of Cortisol - Essay Example

Comments (0) Cite this document
Summary
An author of the essay "Diurnal Rhythm of Cortisol" discusses that necessary to measure cortisol in a patient’s sample to assess the functioning of the adrenal glands in addition to the pituitary gland that is responsible for the initiation of the production of cortisol. …
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER93.5% of users find it useful
Diurnal Rhythm of Cortisol
Read TextPreview

Extract of sample
"Diurnal Rhythm of Cortisol"

Download file to see previous pages The most reliable way of measuring the amount of cortisol is a technique known as enzyme-linked immunosorbent assay (ELISA). There are three forms of ELISA namely competitive ELISA, sandwich and indirect ELISA. Competitive ELISA is the most dependable way of measuring salivary cortisol because it allows for the detection of active cortisol that can compete with labeled cortisol. In this assay, an antibody specific to the antigen being tested is immobilized on a well or a microtitre plate. A mixture containing a known concentration of the labeled antigen and the test sample is then added to the well. The labeled antigen and unlabeled antigen compete for the binding site on the immobilized antibody. Any unbound antigen is washed after which a substrate is added. The substrate reacts with the enzyme to form a colored substance that is then detected and the intensity of the color is measured using an ELISA reader. The intensity of the color is indirectly related to the concentration of the antigen in the test sample. Competitive ELISA was used to determine the quantity of cortisol in the salivary samples. The wells were coated with anti-cortisol antibody at a certain concentration such that a given quantity of antigen bound to the antibodies. A mixture of a known amount of cortisol antigen labeled with peroxidase and the patient’s saliva was then added to the well. The labeled antigen competed with the unlabelled antigen to bind to the immobilized anti-cortisol antibodies in the well. ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Diurnal Rhythm of Cortisol Essay Example | Topics and Well Written Essays - 1000 words”, n.d.)
Retrieved from https://studentshare.org/biology/1633501-diurnal-rhythm-of-cortisol-write-up-report
(Diurnal Rhythm of Cortisol Essay Example | Topics and Well Written Essays - 1000 Words)
https://studentshare.org/biology/1633501-diurnal-rhythm-of-cortisol-write-up-report.
“Diurnal Rhythm of Cortisol Essay Example | Topics and Well Written Essays - 1000 Words”, n.d. https://studentshare.org/biology/1633501-diurnal-rhythm-of-cortisol-write-up-report.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
CHECK THESE SAMPLES - THEY ALSO FIT YOUR TOPIC
Detection of the Diurnal Rhythm of Cortisol Secretion
Cortisol is also released in reaction to the “fight or flight” response in human beings and must be regulated later for normal body functioning (Nelson, 2011).
4 Pages(1000 words)Essay
Personality and Social Psychology Bulletin
It arose from various studies and arguments on what caused higher HPA Axis stimulation. The background to the research was that human beings value social groups and that the body responds in a certain way in order to protect their social self. While some research argued that both physical and psychological stressors can lead to activation of the HPA axis, others argued that the psychological stressors are more pronounced and give profound results.
4 Pages(1000 words)Essay
Circadian Rhythms and Their Importance to Humanise and other Animals
Kumar, Mohan and Kumar-Sharma (2005) found that "arrhythmic [patterns] from both populations lived significantly shorter than the rhythmic ones, suggesting that circadian dysfunction is deleterious to the physiological well beingeven under controlled laboratory conditions" (p.
3 Pages(750 words)Essay
Clocks in the Vasculature
Hierarchically, the circadian rhythm and timing in humans is governed by a master clock in the suprachiasmatic nuclei of the hypothalamus. They function through oscillatory impulses synchronised with time and in coordination with both central and peripheral tissues, and putatively both neural and humoral outputs mediate and result from these oscillations leading to an organised physiological function.
4 Pages(1000 words)Essay
Critical Review Persostent effects of cognitivebrhavioural stress maagment on cortisol responses to acute stress in healthy subjects
It is evident that body's relaxation response must be activated to combat stress. The condition of the stress cannot be prolonged as it could be devastating and induce chronic stress worsening cognitive performance, suppressed thyroid function, hyperglycemia, reduction in bone density and muscle tissues, increased blood pressure and lowered immunity, increased abdominal fat and hence invites all linked (Scott, E).
7 Pages(1750 words)Essay
Epidemiology Assigment
31, Issue 3, pp 333-339. Aim: Cortisol is also known as the stress hormone because of its secretion at higher levels during body’s fight and flight and is responsible for several stress related changes in the body. It is essential to combat stress by inducing
11 Pages(2750 words)Essay
Cortisol affects on Serotonin, Norepinephrine, & Dopamine
Cortisol is produced by the adrenal cortex in response to a hormone known as ACTH. It is also known as a “stress hormone” since it is part of the body’s response to stress especially when the body is in a “fight or flight mode’( Scott 2008).
3 Pages(750 words)Essay
Does stress induced cortisol contribute to central obesity
Obesity is a multidimensional disease that is increasing in prevalence, especially in the Western countries, including the United States and United Kingdom (Flegal, 2002). Many factors have been observed to be related to its etiopathogenesis. One such factor is stress. Stress is thought to influence human eating behaviour.
18 Pages(4500 words)Essay
Human circadian rhythms
Until the physiology is accurately known, appropriate treatment cannot be designed. This article encompasses an overview of clinical disorders that are caused or affected
6 Pages(1500 words)Essay
Biopsychosocial Outcomes for Adopted Adolescents
It is anticipated that 300 students from grade 9 - 12 will be selected from across a variety of high schools within the city of [.. ] in the U.S.A. Salivary cortisol will be collected for two-weeks, and a narrative activity undertaken as well as an Attachment form and a Relationships with Others form.
12 Pages(3000 words)Essay
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Essay on topic Diurnal Rhythm of Cortisol for FREE!
logo footer
Contact us:
+16312120006
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us