Aims of the Practical - Lab Report Example

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Aims of the Practical The aim of these practical is clearly stated in the introduction of the practical lessons. It is clearly indicted that genes from a donor can be used to litigate some other genes in a recipient. This will assist in developing strong bonds in the recipient…
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Download file to see previous pages In the end, the recipient would have an enhanced combination of genes, as they have been donated from the donor to the recipient (Carson, Miller and Witherow, 2011, 66). Therefore, research and pharmaceuticals have an aim of quantifying the appropriate actions that should be used in strengthening a gene by fusing some genes from a recipient to a donor. The aim of this practical is to associate the transportation of genes from one form of DNA to another combination. The recombination of the DNA is basically used to ensure the recipient is at a better state than the previous state. Therefore, finding an appropriate approach towards making a better solution in strengthening gene combination and DNA is a priority in this practical. For instance, when two genes are fixed together, the bonding creates a strong combination. With such a combination, it is evident that the recipient will be at an enhanced position in the daily functionality. It is considered that fusing two genes leads to enhanced activities in the DNA, which is appropriate in keeping a highly functioning gene combination. Materials and methods There is a wide variety of materials that should be used in this process. The materials are to be readily available, to show the importance of different combinations especially in the DNA field. The initial material that will be used in this practical is the FtsZ gene. This is a single celled alga that is indicated by the Pleurochrysis carterae (Pc-ftsZ). This is a highly active protein that is believed to be the vital structure being the division of mitochondrion in the body. The protein is solely responsible for highly initiating the reaction of the mitochondria to multiply at a higher rate. Therefore, using this material in the reaction will be dominant in making an increase in the mitochondria division. The second material to be used in this experiment will be a low-copy of plasmid pProEX. The low copy plasmid will be used in comparison to the second copy of plasmid. The third material is the high copy plasmid, which will be used with the pProEX (Carson, Miller and Witherow, 2011, 31). Inserting the pProEX into the high copy of the Plasmid will be the practical of showing the reaction of the two. The other materials to be used in this experiment are gathered pBluescript KS II which is positive (+). This is a material that is gathered from E. Coli. These will be followed by a double restriction of two enzymes, namely BamHI and HindIII. The reaction will lead to a release of FtsZ, which is also vital in the experiment. After insertion of FtsZ, it will be followed by isolating pBKS II DNA, which is done by inserting a restriction enzyme. As part of the reaction, a catalyst will be used. The catalyst in this experiment will be alkaline-phosphate (AP), which is also a treatment for the lenealised plasmid. Another function of the AP will be preventing any partially digested plasmid from re-circulating. Results Plasmids are considered to be vital in helping bacterial hosts, in that they are useful in preventing bacterial infections. They are useful in resistance to antibiotics and degradation of organic complexes. Therefore, it is a positive maneuver for the plasmids to be increased in their production. Reproduction of the genes in the combination will obviously come out with positive results. First, the body will be well prepared to deal with organic complexes. ...Download file to see next pagesRead More
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