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Results chapter (MICROBIOLOGY specialists only) - Essay Example

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They are Wild Type Mice (WT), EGR 2 –transgenic Mice and EGR2 Knock out Mice. The Knock out mice and the spleen was used for Micro array analysis technique, Thymus for RT-PCR analysis and…
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Results chapter (MICROBIOLOGY specialists only)
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Download file to see previous pages Each T-cell receptor has a specific unique peptide: Major Histocompatibility Complex with them. The Spleen contains lymphocytes and another type of white blood cells called Macrophages. Thus spleen is also the best source of lymphocytes. The T-cells are usually divided into two major Subsets- T Helper cells and T-Killer cells. The T-helper cells are called as CD4+ cells and T-killer cells are called as CD8+ cells. Early growth response gene – 2, a zinc finger transcription factor is expressed in the CD44 cells. It also controls their activation and proliferation. In the absence of this EGR gene -2 at CD44high + cells, and not CD44low + cells, the T cells were found to be hyper reactive and hyperproliferative in vivo. (Zhu et. al, 2008)
In this experiment, two types of cells were used for FACS research, Wild type and EGR-2 Transgenic mice. The cells extracted from the spleen are separated for the lymphocytes and then mixed with 1 μl of the antibodies. The antibodies used are CD3 P (Phycoerythrin)+CD4 F(Fluorescein), CD3 P+CD8 F, CD4 F+CD8 P. (, 2009). The number of the positive cells were calculated for the two types and compared with each other.
The wild type and the EGR-transgenic mice spleenocytes were stained with CD4 (FITC SL) + CD8 (PE SL). The results have shown that the wild type mice has the normal count. The CD4 positive cells are greater than the CD8 cells normally. In transgenic mice, the CD4 and CD8 cells counts are nearly the same. The EGR-Transgenic mice are found to have greater CD8 cells than the wild type.
The wild type and the EGR-transgenic mice spleenocytes were stained with CD3 (PE SL) + CD4 (FITC SL). The results indicate that There was no characteristic difference in the CD3 positive cells in both wild type and EGR2. Whereas the CD4 positive cell count was nearly double in the wild type when compared to the EGR2 Tr.
The staining of all the three type spleenocytes with CD4 FITC +CD3 PE, has showed that ...Download file to see next pagesRead More
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