Photo-Induced Cytotoxicity and Bio-Distribution of Prostate Cancer Cell-Targeted Porphyrin - Article Example

Vitro studies of porphyrin sensitizers conjugated to peptides that contain a cell-penetrating peptide (CPP) show a significant increase in cellular uptake and phototoxicity when compared to unconjugated porphyrin (Inder Sehgal). It results from susceptibility to enzymatic hydrolysis accompanied by a lack of tumor specificity. Measurement of electronic absorption spectra was done using Perkin-Elmer Lambda 35 UV-vis spectroscopy. Mass spectroscopy was performed using Bruker Proflex III MALDI-TOF mass spectrometer.

Synthesis of peptide conjugate 3 was examined from 5-(p-aminophenyl)-10, 15, 20-triphenyl porphyrin by reaction with di-glycolic anhydride. PC-3m cancer cells were put through 10000cell/cm2. The sample of prostate cancer cells was then exposed to porphyrin increasingly for dark cytotoxicity and phototoxicity. Mice were made use of in the experiment on the compounds (Inder Sehgal). Inder Sehgal et al. (2008) examined five porphyrin-peptide conjugates within vitro using PC-3M of the human prostate.

In addition, they compared FDA-approved purified hematoporphyrin derivative performance to the second-generation PDT photosensitizer, mTHPC. The results showed high photo-toxicity and consequently small dark cytotoxicity. The outcome ascertained that porphyrin HIV-1 is highly promising Tat conjugate two which can be employed to treat human prostate cancer. It achieves this by maintaining a favorable vitro cellular uptake. Porphyrin-CPP showed less toxic levels when compared to FDA-approved purified hematoporphyrin derivative.

Moreover, its selectivity is more than hematoporphyrin derivative. Ultimately, they observed that coupling the long-wavelength absorbing porphyrin photosensitizer to HIV-1 tat (48-60) in a sequence would help to enhance PDT of the prostate tumors (Inder Sehgal).