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Human oocyte cryopreservation can be particularly helpful to women at the risk of losing their ovarian function to premature menopause, surgery or cancer treatment (Trad et al. 1570). However, the survival rates of the oocytes have not been adequate, and this method has not been…
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Download file to see previous pages In order to attain the primary aim of the research, questions such as “what is the median temperature at which intracellular ice formation occurs in human oocytes?” “How readily does intracellular ice formation (IIF) occur in human oocytes?” and “what is the effect of extracellular seeding on human oocytes?” (Trad et al. 1572-73).
These questions were answered through an experimental design that used failed-to-fertilize and fresh oocytes with a germinal vesicle and polyspermic eggs. This design included visualization of how IFF first occurred at a cooling rate of 120OC/min using a programmable thermal microscope stage attached to a video microscope (Trad et al. 1574). It also involved execution of extracellular seeding at a cooling rate of 0.2 OC/min in order to decrease the occurrence of IIF and in turn increase survival rates of the freeze-thawed human oocytes. The design also used distinct cryoprotectants and decreased the median temperature in both mouse and human oocytes (Trad et al. 1575).
The study portrayed that IIF occurs enthusiastically in human oocytes, and detrimental IIF can be avoided, and survival rates maximized. The results portrayed a 78%, 33% and 0% occurrence of IIF, and the 24 h post-thaw survival rate was 32%, 56%, and 93% respectively (Trad et al. 1576).
Trad, Fouad S., Mehmet Toner, and John D. Biggers. "Effects of cryoprotectants and ice-seeding temperature on intracellular freezing and survival of human oocytes." Human Reproduction 14.6 (1998): 1569-1577. ...Download file to see next pagesRead More
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