Microbiology: Unknown Organism - Lab Report Example

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The paper tells us about the research which purpose is to identify an unknown microorganism by performing a series of biochemical tests on a pure bacterial culture.Two culture tubes containing sucrose broth and lactose broth were inoculated with a pure culture of the unknown microorganism and incubated at 37oC.
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Microbiology: Unknown Organism
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"Microbiology: Unknown Organism"

In the paper we can see the test results did not perfectly match those of Proteus vulgaris on the chart. The differences in the casein hydrolysis test were probably due to due to faults in culture preparation, skim milk agar preparation or in the course of the experimental process. The differences between unknown and all other bacteria were greater than the differences between the unknown microorganism and Proteus vulgaris. In addition, the observed traits match those of documented traits of Proteus vulgaris. Urea hydrolysis: The assigned microorganism was inoculated into a tube containing urea broth. A color change of the PR indicator from orange to pink indicated the presence of urea.
We have every reason to believe that the explorer has maked a good and not easy work to understend the life of the substance and to do all the experiments.
At the and of the research the paper says that the area around the unknown bacteria in the starch agar medium was clear after the addition of iodine. This revealed that the unknown microorganism produced the enzyme amylase, which was responsible for the hydrolysis of starch into single units of glucose. A red ring was formed on top of the reagent tube containing tryptone medium. This was an indication of the production of the enzyme tryptophanase by the unknown microorganism. Tryptophanase was responsible for the breakdown of tryptophan into indole and pyruvic acid. The urea broth changed to pink because the unknown bacteria produced urease that decomposed urea into ammonia and carbon dioxide. Read More
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