StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...

Identification of unknown - Lab Report Example

Comments (0) Cite this document
Summary
The use of diagnostic bacteriology has made it possible to identify unknown bacterial cultures from clinical and environmental materials based on their metabolic or biochemical characteristics. Specific enzyme function and cellular respiration of a bacteria is key in the…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER93.5% of users find it useful
Identification of unknown
Read Text Preview

Extract of sample "Identification of unknown"

Download file to see previous pages We were provided with number coded 24- hour broth culture containing a mixture of any two bacteria (gram +ve or gram –ve), 1 EMB plate, 1 blood agar plate, several inoculating loops and TSA slants. Other materials (mediums and reagents) required were requested from the laboratory technician depending on the type of tests that were to be carried out to facilitate in identifying the unknown bacteria.
There were several tests to be carried out to identify the unknown bacteria. On receiving the less than 24 hours broth culture which contained two types of bacteria (G +ve and G- ve), I prepared a stock culture for culture purification. Labelled the bottom of blood agar plate (nonselective medium) with my identification codes, then chose one of the unknown culture, opened the petri dish carefully without placing the top of dish down on the bench. I used the inoculating loop to gently swipe across an area of the bacteria to transfer from the dish to the loop and gently made three successive streaks of the bacteria onto the agar surface of the labelled plate. Incubated the plate at 35o C for almost a week, I kept checking it regularly until single colonies had grown. Then I made a subculture of a single colony on TSA slant, incubated it at 35o C after good growth, I removed from the incubator and stored at room temperature.
After acquiring the individual colonies, I performed a gram stain test using EMB (eosin Methylene blue) and lactose agar plate. Collected bacterial colony from and streaked on the EMB plate and waited for some days to observe the result. The unknown bacteria was Gram negative. With G –ve result I had to carry more tests: Oxidase test, Lactose fermentation test, Indole test, Bile Esculin test, Motility test, Methyl Red -Voges Proskauer test, and Lysine decarboxylase test.
Oxidase test: Obtained a liquid culture stock of my gram ...Download file to see next pages Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(“Identification of unknown Lab Report Example | Topics and Well Written Essays - 1000 words”, n.d.)
Retrieved from https://studentshare.org/medical-science/1669210-identification-of-unknown
(Identification of Unknown Lab Report Example | Topics and Well Written Essays - 1000 Words)
https://studentshare.org/medical-science/1669210-identification-of-unknown.
“Identification of Unknown Lab Report Example | Topics and Well Written Essays - 1000 Words”, n.d. https://studentshare.org/medical-science/1669210-identification-of-unknown.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Identification of unknown

Isolation and identification of unknown bacteria

...?ISOLATION AND IDENTIFICATION OF UNKNOWN BACTERIA INTRODUCTION Identification of bacteria as a causative agents of diseases, epidemics, contaminated food and other sources of infection and illness is a process that has growing significance in the modern world. Bacteria are a causative agents for many diseases in humans and domestic animals that and can be a cause of significant health impact on the human population (Scallan et al. 2011). Bacteria also can contaminate and impair the process of food production and packagind that can lead to significant economic impact but more importantly can be a source of more widespread morbidity in human population and a mechanism of generation of widespread epidemics. With the appearance...
11 Pages (2750 words) Lab Report

Identification of Unknown Bodies using DNA

...?Running head: IDENTIFICATION OF UNKNOWN BODIES USING DNA Identification of Unknown Bodies using DNA Insert Here Insert Affiliation Here Identification of Unknown Bodies using DNA The identification of an unknown body is perhaps one of the most daunting tasks for forensic detectives. The obscurity of the task further multiplies when the bodies to be identified belong to victims of mass disasters, catastrophes, gruesome murders and accidents, in which they are deformed or decayed to a large extent. Traditionally, fingerprints have been used for the forensic identification of individuals for a long time, yet they have their own limitations. They are easily deformed due to injuries and scratches, and even suffer from the risk of being...
7 Pages (1750 words) Research Paper

Identification

... IDENTIFICATIONS Why there is no socialism in the U.S? Socialism refers to a system wherebythere is cooperation between or among several institutions or individuals towards managing economic activities productive to the nation. The United States, unlike other countries such as Japan and Germany, has not employed this system of running the economy. Though the system has worked very well in the above mentioned countries, the United States continues to thrive well without its enforcement. Politically, socialist parties in the United States began fairing very poorly in the run up to the general elections. For instance, in the year 2008, socialist candidates and their parties received less than two of the three socialist candidates. This is way...
10 Pages (2500 words) Essay

Identification

... Supervisor: History and Political Science identification Die Welle According to Weber, the real politics is far much different from what is taught in the lecture room. In classroom, there is no one present to oppose the imprints of a teacher on the student like the case is in a political arena. In the political world, one does not hide his or her standpoint like it happens in lecture rooms. It is therefore inappropriate to state that democracy is absolutely perfect. As matter of fact, it has been argued that democracy can also be a bad form of government (Ingram 2). This has been reinforced by the fact that some nations that are non-democratic have managed to achieve impressive economic production. Fascism has been successful in a number...
10 Pages (2500 words) Essay

Identification

... History Dixiecrats The Rights Democratic Party also known as the Dixiecrats played a significant role in the United States’ history. Dixiecrats was a movement party was organized in 1948 by the Conservative White Southern Democrats organized the Dixiecrats, which was committed to enhance the change to states’ rights and preservation of segregation. Still, it aimed to deter racism and discrimination that had affected so many people. The roots of this party dates back to the introduction of the pro-labor reforms, which were enacted by the Fair Labor Standards and the Wagner act. The Dixiecrats was the strongest movement in the southern states with the largest African American populations. Most members establish the need to be part... History...
5 Pages (1250 words) Essay

Biochemical identification of unknown bacteria

...LAB REPORT Biochemical identification of unknown bacteria Unknown ID 001 Nerjes Alsalman 3/18/09 Biochemical identification of unknown bacteria RESULTS: Morphological characteristics: Nutrient Agar Slant: The tube in 37oC: The amount of growth was slight, white in color, and had effuse form of growth. The tube in 25oC: The amount of growth was slight, white in color, and had Echinulate form of growth. Nutrient Agar plate: The colony characteristics on the plate looked like convex and round. Nutrient Broth: The surface and subsurface growth was flocculent, and the amount of sediments was granular. Fluid thioglycollate medium: The bacterium was facultative. The growth of bacteria was almost everywhere which means...
4 Pages (1000 words) Lab Report

Identification

...Ghaleb Saad Identification Task My is Ghaleb Saad and I was born in Wadi Aldawasir, Saudi Arabia. I grew up in Jeddah, Saudi Arabia. I graduated from King Abdulaziz University in 2009 with the course in Industrial Engineering. I took some courses to improve the following skills: Course in Leadership & Communication Skills during the period February 16, 2008 to June 3, 2008. Course in The PIC Microcontroller by the ECE Department from November 2008 to November 29, 2008 Training course on LabVIEW on March 5, 2009. Training course on : How to connect with yourself and with others in the work environment on December 31, 2008. I have had some work experiences in two positions which are at Al-Adwa’a Institute as a training and development...
1 Pages (250 words) Essay

Unknown

...Research Proposal Salem Witchcraft Trial of 1692 To make the research paper about America’s history relevant, it would make an interesting writeto delve into one of America’s darkest history. America too underwent a stage of religious fanaticism and fatalism just like her rabid critic today. This proposed research is relevant in today’s socio-political environment because her practice of freedom both in religious practice and speech offends many of its critic in today’s contemporary world yet she herself at some point were also as fatalistic as they. The proposed research is about the Salem Witchcraft of 1692. This interesting because in 1692, witchcraft was considered a crime in the United States and anybody who is tried... Proposal...
1 Pages (250 words) Thesis

Identification of unknown Bacteria

...Identification of unknown Bacteria The purpose of the experiment was to identify unknown bacteria from mixed culture #18 using biochemical and morphological test: The tests done include: Culturing and Gram staining for Morphology. The biochemical tests were: Lactose fermentation, Tryptophan digestion (Indole), Orinithine, methyl red and mannitol test. Basing on the results the most likely bacteria were: for the unknown A, was Klebsiella pneumoniae which is a typical rod shaped bacterium, a Gram-negative, lactose fermenting, facultative anaerobic, encapsulated and non-motile. For the unknown B: It was likely to be Salmonella Typhimurium bacteria which is a typical nonspore-forming rods, facultative anaerobic, motile and negative...
6 Pages (1500 words) Research Paper

Unknown compound

...Identification of Cations and Anions in a Solution Chemistry Lab Report March 4, Qualitative analysis and conductometry, which is a measurement of electrolytic conductivity, are the commonly used methods for identifying cations and anions present in unknown solutions. The two methods were employed to achieve the main objectives of this experiment, which included identification of cations, and anions present in an unknown solution. When employing qualitative analysis in determination of identity of unknown solution, similar tests are performed on a known and unknown sample and their characteristics are matched in order to determine the identity of the unknown. In this experiment, the flame test of the unknown solution gave a pink color...
10 Pages (2500 words) Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic Identification of unknown for FREE!

Contact Us