Staphylococcus and streptococcus - Lab Report Example

Comments (0) Cite this document
Name Lab Instructor Title Date Identification of unknown Bacteria Introduction The main objective of the experiment was to Identify the Unknown Isolated bacteria in the sample using morphological and biochemical test: The following tests were done: For Morphology, Culturing and Gram staining was done…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER95.5% of users find it useful
Staphylococcus and streptococcus
Read TextPreview

Extract of sample
"Staphylococcus and streptococcus"

Download file to see previous pages Botulinum, C.Perfringen among others and Staphylococcus spp and streptococcus spp. Bacillus spp are typical endospore, competent naturally, gram-positive, aerobic or facultative aerobic bacilli .Rode shaped. These tests have been explored in the next section. Results Gram staining Commonly, Gram staining is technique employed in the two broad groups of bacteria differentiation and it’s based on the different constituents of the bacteria cell wall. The technique is able to distinguish between Gram negative and Gram positive bacteria groups by red or violet coloring of the cells. Violet staining indicate Gram positive bacteria because of a thick layer of peptidoglycan presence in the bacteria cell walls, hence the crystal violet is retained by these stained cells. On the other hand, red staining, indicate Gram negative bacteria and this is due to a thinner peptidoglycan wall that never retains the crystal violet during the process of discoloring. In the test the bacteria were Gram positive, the primary stain was retained and the secondary stain was lost resulting to violate appearance after viewing under a microscope. Culturing The incubation was both with incubation on agar and broth media at a temperature of 37 oc and 25 oc Techniques A spec­i­men loop was got then streaked on one sec­tion, flamed and cool, later turned at 45o then streaked for a 2nd time, that over­lap­ped the first streak.  It was repeated for third and fourth time.  Iso­lated regions were got in the third region with large growth amount, irregular growth form and with elevated colony. Incubation temperature was at 37 oc. When this isolate was grown on agar plate at 37 oc, there was a lot of growth with dark colored colonies; growth form was circular, with flat, filamentous margin. Alternatively when it was grown on agar at 25 oc, the amount of growth was spread out, growth form was irregular and flat, and margin was flat. Growth in broth media at 37 oc the amount of growth was that there was a membrane on top of the broth; growth pattern was aerobe with oxygen on top. But in broth media at 25 oc there was no growth seen. Biochemical tests: Carbohydrate Fermentation    Some bacteria identification is aided by identifying the type of nutrients it can utilize as well as the type of end products in the process that will be produced. These characteristics are regulated by the bacteria enzymes produced. Furthermore, this enzymes produced are genetically regulated, the sugars pattern fermented may be unique to a specific strain or species. The products of fermentation are normally acid such as acetic acid, lactic acid, etc, neutral such as ethyl alcohol etc, or gases like hydrogen, carbon dioxide, etc. In the experiment, the determination of the sugar fermentation products of the unknown organism given was by first preparation of carbohydrate fermentation broth at pH 7.4. The broth contained any of the following essential ingredients: 0.5% to 1.0% of the carbohydrate to be tested that included lactose, glucose, Sucrose maltose and starch, nutrient broth and the phenol red pH indicator. The light red color nutrient broth is able to support most organisms’ growth regardless of being able to ferment sugar or not. The unknown test organism was inoculated into a broth having the mentioned above test sugar then later incubated. Production of enough acid products was indicated by a bright ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“Staphylococcus and streptococcus Lab Report Example | Topics and Well Written Essays - 1000 words”, n.d.)
Retrieved from https://studentshare.org/biology/1475918-staphylococcus-and-streptococcus
(Staphylococcus and Streptococcus Lab Report Example | Topics and Well Written Essays - 1000 Words)
“Staphylococcus and Streptococcus Lab Report Example | Topics and Well Written Essays - 1000 Words”, n.d. https://studentshare.org/biology/1475918-staphylococcus-and-streptococcus.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document
Staphylococcus and Streptococcus

The author states that microbes can be classified into various groups depending on their morphological and biochemical features. The Lancefield grouping is a method of clustering catalase-negative, coagulase-negative bacteria according to the carbohydrate structure of bacterial antigens present on their cell walls.

4 Pages(1000 words)Lab Report
Selective Media
Media has essential nutritive components that support bacterial growth. Microorganisms can acquire nutrients from the media to sustain their growth. Some media have substances that may inhibit or enhance the growth of some bacteria; hence such media is
4 Pages(1000 words)Lab Report
Simple staining and Gram staining
It was done through gram staining and simple staining procedures. These two procedures branded us with the ability to comprehensively distinguish the distinct nature of bacteria based on the gram
3 Pages(750 words)Lab Report
Using this model, we aim to understand the extent of change in the GI tract microbial count of streptozotocin injected diabetic rats compared to in normal rats based on a comparative assay of their glucose content, lactate content and the ratio of the GI tract to the body weights.
30 Pages(7500 words)Lab Report
In the present study, the unknown microorganism present in the Probiotics, maozrella cheese and camembert cheese were analyzed using the biochemical tests such as catalase, oxidase, latex agglutination, Gram’s staining and string test. From the biochemical results it was identified that the unknown microorganisms present in the given samples of probiotics, mozeralla cheese and camembert cheese were Lactobacillus sp, Streptococcus sp and Penicillium sp.
6 Pages(1500 words)Lab Report
Antibiotic sensitivity
This is done using the Kirby and Bauer method. The paper is divided into three sections. Section one is an introduction to the process of bacterial sensitivity to various antibiotics and various methods used in testing for sensitivity.
4 Pages(1000 words)Lab Report
Dichotomus key gram postive bacteria coccus
It is also during this initial test that we can also check the shape of the bacteria being identified. We know that Staphylococcus and Micrococcus are circular in shape, unlike the gram-(+) Bacilli that is
1 Pages(250 words)Lab Report
Scientific Concepts and Methodologies
Poor sewerage is analogous to intestinal infection inflicted by these microorganisms. Microorganisms in water are therefore supposed to be understood to avert possible disasters in future. Microorganisms are in
4 Pages(1000 words)Lab Report
Culture dependent vs culture independent methods
al species detected by 16 S rRNA included, Actinomyces, Corynebacterium, Rothia, Propionibacterium, Atopobium amongst other highlighted by the graph above. As for culture-dependent bacteria, staphylococcus forms the predominant group. It is however worth noting that majority of
2 Pages(500 words)Lab Report
Staphylococcus has various types; one important one is the methicillin resistant staphylococcus aureus. The organism causes nosocomial or hospital acquired infections.
5 Pages(1250 words)Research Paper
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.
Let us find you another Lab Report on topic Staphylococcus and streptococcus for FREE!
logo footer
Contact us:
Contact Us Now
FREE Mobile Apps:
  • StudentShare App Store
  • StudentShare Google play
  • About StudentShare
  • Testimonials
  • FAQ
  • Blog
  • Free Essays
  • New Essays
  • Essays
  • Miscellaneous
  • The Newest Essay Topics
  • Index samples by all dates
Join us:
Contact Us