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The micro organisms are ubiquitous in nature. The presence of some of these micro organisms in the food and in human body will lead to health effects. Hence the identification and characterization of these micro organisms is of vital importance. These micro organisms can be identified based on some special characteristics exhibited by them. The biochemical testing method is designed to identify the ability of the micro organisms to produce some specific metabolites. Most of the micro organisms invade the respiratory tract, genitourinary tract and gastrointestinal tract. Here the above mentioned methods were used to identify the given unknown micro organism. The unknown micro organism was found to be Pseudomonas aeruginosa.
The identification of the bacteria in the given sample can be done by gram’s staining, biochemical and physiological tests. The colony morphology is the first step in the identification process. The size, shape and the gram’s reaction will give us an idea about the bacteria. Based on the Gram’s stain, we can classify the bacteria as gram positive or gram negative. After the classification the biochemical tests are done. The basic biochemical tests include: Indole production test, Methyl Red test, Voges Prosker Test, Citrate test, Catalase test, oxidase test, Urease test, Triple sugar test, Motility staining test and Morphology identification. These tests will give us, the idea of what type of bacteria it is. (Todar 2008). The identification of the physiological characteristics by anaerobic test and motility test will also give us better idea regarding the nature of the bacterium. Then using the Bergey’s Manual of systematic Bacteriology Volume 1, we can identify the bacterium. (Shimeld and Rodgers 1999), (Harley 2004).
1. Colony Morphology: Colony morphology is an important tool in the identification of the bacterial cultures. The bacteria are classified based on their size, forms,
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The author analyzes the importance of accurate identification of bacteria and the necessary materials and methods. He shows newer procedures of rapid identification of bacteria like mass spectrometry and computional analysis, microarray-based identification and other that allow faster and affective method of identifying bacteria and other microorganisms.
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The paper gives a review of E-Coli transformed by plasmid DNA using a rapid method and can be categorized into four stages. The first is Pre-incubation stage. In this the cells are immersed in a cat ions solution and incubated at 0ºCThis immersion is supposed to help the negatively charged phosphates of lipids in the E-Coli membrane.
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When employing qualitative analysis in determination of identity of unknown solution, similar tests are performed on a known and unknown sample and their characteristics are matched in order to determine
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