DNA Barcoding - Lab Report Example

Comments (0) Cite this document
This paper outlines that DNA barcoding is a standardized method of identifying the organism by using small segments of their DNA. Essentially it is a DNA based identification method which among provides a more advanced species identification as compared to the traditional species identification…
Download full paperFile format: .doc, available for editing
GRAB THE BEST PAPER91.6% of users find it useful
DNA Barcoding
Read TextPreview

Extract of sample "DNA Barcoding"

Download file to see previous pages The protocol utilizes short sequences of organisms to characterize them. These oligonucleotides fall in positions in the genome which are agreed upon and its standard for a particular genome of interest. The DNA barcode sequences are also rather short in comparison to the entire genome and can be extracted with relative ease utilizing cheap methods. For instance, the cytochrome C oxidase subunit 1 mitochondria region (COI) has in the recent times being the standard barcode region for higher animals. One define characteristic of the DNA barcode is its commonality within a species (within species) and variations among species (without species). ie for a selected DNA barcode of a particular species there exists ranging differences and these differences are minor in individuals of the same species to gurantee the sequence segment to be used as a barcode.
In obtaining the DNA for branding, the mayfly should be killed in a ‘DNA friendly fashion’ by avoiding the use of preservation agents such formalin which may degrade DNA. Genomic DNA is isolated via the fast DNA extraction method from fresh or frozen specimens. Combination of Chelex protocol with Proteinase K may rule out the need for tissue disruption while guaranteeing the release of DNA leaving chitinous material left intact. PCR amplification is done with an optimal primer specific for the barcode region. The barcode products obtained from the PCR are in most instances sequences bidirectional and later deposited in the barcode reference library.  ...Download file to see next pagesRead More
Cite this document
  • APA
  • MLA
(“DNA Barcoding Lab Report Example | Topics and Well Written Essays - 750 words”, n.d.)
Retrieved from https://studentshare.org/biology/1591464-dna-lab-report-2
(DNA Barcoding Lab Report Example | Topics and Well Written Essays - 750 Words)
“DNA Barcoding Lab Report Example | Topics and Well Written Essays - 750 Words”, n.d. https://studentshare.org/biology/1591464-dna-lab-report-2.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document



...chromatids separate, producing haploid cells with unreplicated chromosomes. Ideally, 4 daughter cells are produced per meiosis of a –gonium, and this is what happens in the production of sperm cells. However, in the case of female gamete formation, 2 daughter cells (1 from meiosis I and 1 from meiosis II), only 1 oocyte is produced from a cycle of meiosis (Campbell and Reece, 2002). DNA replication occurs in preparation for cell division How does DNA replicate? A part of the double-stranded DNA (dsDNA) unwinds, allowing DNA polymerase and DNA ligase to get into what is known as the replication bubble. The DNA polymerase adds the...
4 Pages(1000 words)Essay


...? The Unique Qualities of Blood, DNA, and Mitochondrial DNA Your The scene is so common its almost cliche: the courtroom drama climaxing with the identification of the killer by irrefutable DNA samples presented in court; yet what are these samples actually made of, and how do lab technicians utilize the unique qualities of human tissue to make specific identifications? The answer lies in deoxyribonucleic acid (DNA), and its unique composition. Like a fingerprint, each person has a unique DNA sequence that contributes to their individuality (NIH). DNA then goes on to encode for unique proteins that act as markers in the blood, which help lab technicians make specific identifications (NIH). Similarly, mitochondrial DNA can be used... as a...
3 Pages(750 words)Essay


... DNA: The Basis of Life The question of “What is life, and what is it made of” has been asked to the point of making it cliche,if not ubiquitous, yet a multitude of answers persist. Life could be defined as a potential, as a response to external stimuli, or even as an indefinable feeling, yet none of these answers attempts to solve the question of what actually drives the biological engine of our bodies that keeps us breathing, pumping blood, and absorbing food. On April 25th, 1953, the now famous scientific pair of Watson and Crick attempted to finally answer this question (Fredholm). They claimed that an elegant molecule called deoxyribonucleic acid, or DNA, was the “God Molecule”, the blueprint for every protein in our body... (and the...
5 Pages(1250 words)Essay

DNA Fingerprinting

...DNA Fingerprinting DNA fingerprinting is a laboratory procedure that has proved very useful in personal identification. With its wide applications, it is causing a revolution in the modern world. Typing of DNA presents unique profiles for each individual except identical twins. The high rate of variation results because DNA fingerprinting relies on non-coding hyper-variable sequences to produce a unique pattern of bands for each individual. DNA profiling relies on the discovery of a broad range of restriction enzymes and their specificity. DNA typing has a wide range of applications from paternity testing, criminal investigations, and...
5 Pages(1250 words)Research Paper

DNA Fingerprinting

...DNA Fingerprinting Introduction DNA as we all know is the basic building structure of every living thing present on earth. It has been scientifically proved that it is the DNA that gives us our characters, both phenotypic and genotypic. If the there is some sort of problem i.e. mutation in the DNA, it would lead to altered characters or even lead to abnormalities (Burke 1991). The DNA sequence of every individual is specific and is generally passed on from parents to their offspring. The basic structure of a DNA consists of four Nucleotide bases i.e. Adenine, Thymine, Guanine and Cytosine. These four bases pair with each other in a...
4 Pages(1000 words)Essay

Describe and discuss the diverse ways in which the development of second generation sequencing technologies has extended the fie

...?Second Generation Sequencing extending DNA Barcoding beyond Sanger Sequencing Literature Review Early sequencing technique came into existence in the 70s, and involved the use of bases that are chemically altered known as, dideoxy. Dideoxy terminated DNA fragments that were synthesized in different bases, for example, A, C, T or G. The fragments then, underwent size-separation for purposes of reading the sequence of DNA. On the other hand, DNA barcoding involve characterizing or categorizing species by applying short DNA sequence using an agreed position concerning the genome. The sequence related to DNA...
5 Pages(1250 words)Essay

DNA Mutations

...Mutation is the process that involves the change or damage of a DNA to extend so as to alter the genetic setup of that gene (Blamire ,2000). The relationship between genes and DNA are best understood by mutation studies. The effects of large deletions and arrangement in a segment of DNA may result in the loss or gain of a gene or so a function. Resultantly the mRNA transcribed from that particular changed or damaged gene will carry an entirely different genetic message (Blamire ,2000). The polypeptide created as a result of the translation of the changed mRNA will carry a different sequence of amino acids. The function of the protein made by this polypeptide will be changed or lost as...
2 Pages(500 words)Essay


...DNA DNA represents the genetic material within a cell that provides the basic blueprint for living cells, somewhat like a program for creating biological structures. Though DNA has received much attention in the media in recent years, it is important to note that the discovery and implementation of most DNA techniques only occurred as recently as the middle of the twentieth century. Understanding of the history, structure, and function of DNA is critical to modern science. Though DNA had long been known to exist is the cell, the structure and importance of the compound was not fully understood until 1953 when Francis Crick and James...
3 Pages(750 words)Term Paper


...DNA and protein Database assignment Part 1) Nucleotide numbers constituting the coding sequence of: E1 alpha: NM_000284.3  3390 E1 beta: NM_000925.3  1544 (2) Number of exons and introns present in the genomic sequence of: E1 alpha: NM_000284.3  Exons = 11 Introns = 10 E1 beta: NM_000925.3  Exons = 10 Introns = 9 (3) E1 Beta sequence – BLAST results (Description of three sequences with highest homology): Name Pyruvate dehydrogenase (lipoamide) beta (PDHB) Pyruvate dehydrogenase (lipoamide) beta (PDHB) Pyruvate dehydrogenase (lipoamide) beta (PDHB) Accession number NM_001131433.1 NM_001246418.1 NM_001195394.1 Organism Pongo abelii (Sumatran orangutan) Pan troglodytes (chimpanzee) Macaca mulatta (Rhesus...
3 Pages(750 words)Coursework

DNA Barcoding Invertebrate Lab Report #1

...Biology Topic: lab report on DNA barcoding Introduction: DNA barcoding provides a versatile tool for ification of organism based on DNA taxonomy and delimitation and ‘discovery’ of new species. Sequences that are used as barcodes are usually those that are unique and conserved within a species. In DNA barcoding, usually a four staged approache is engaged. The first stage is to obtain a specimen which may be from from tissue or culture collections. In the second stage, laboratory analysis which involves extraction of genetic material to obtain DNA batcode sequence is done. These...
2 Pages(500 words)Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Lab Report on topic DNA Barcoding for FREE!

Contact Us