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Data analysis - Essay Example

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The experiment uses AKT to represent the upper blotting while the phosphor-AKT represents the lower blotting. It proves that the Phospho-Akt Antibody…
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Data analysis
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Download file to see previous pages The Experiment of Western blotting analysis for the samples of NIH/3T3 cells, whether having been treated in PDGF or not, uses the Phospho-Akt (Ser473) for the preparation of the upper blotting and Akt 9272 for the lower blotting. It has to present the results in a tabular form, the first column being PGDF, the second being AKT and the third column being Phospho-Akt.
This was a time series practical activity to stimulate the cells at various durations from 0 to 120 minutes. Its independent variable was the PGDF. There are two blots to analyze, Ser473 of Akt as the upper blot (using phospho-Akt antibody) and phosphor-Akt (using Akt antibody) as the lower blot and. It also involved using gels to conduct the molecular weight marker. The top band corresponded to the measurement of 76 kDa, while the middle band corresponded to 57 kDa. The lowest band thus corresponded to 47 kDa.
The growth factor analysis shows that proteins facilitate growth at different rates under different conditions. The most relevant protein analysis procedure in this kind of experiment is the florescent microscopy.
From the principles of cytometry, this experiment conducts the flow cytometry experiment with the labeling of Jurkat cells and the labeling of Phospho-Akt ser 473antibody. It uses three categories of cells, untreated, treated cells and control experiment (Burgering and Coffer 1995, p54). All the cells are labeled in three different colors as they appear in the graph above. The red line is the control experiment, green is treated and blue is ...Download file to see next pagesRead More
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