On a graph draw your suitably-labelled log concentration – response curves and list the two EC50 values (with units) obtained by your set of 3 students (i.e. for the agonist in the absence and presence of the unknown drug).
The graph shows that with acetycholine, there was…
Download file to see previous pages...
Fill in the table (see next page) for all 4 types of agonist/antagonist combination used. Using appropriate statistical tests on the class data, examine whether in each case the presence of the antagonist increased the log EC50 value of the agonist significantly (in other words, made it less negative)*.
(ii) With reference to the class practical results in the table (page XX), describe in detail what the experimental evidence is for your answers to question 3(i). This justification of your conclusions is a very important part of this practical write-up.
X- Due to the introduction of the drug x, there was contraction response on acetycholine as seen by the less negative values after the introduction of the drug. He drug acts on cholinergic nicotinic receptors, where acetycholine derives it actions.
Y-As a result of the introduction of the drugs, the histamine action was strongly altered as seen by less negative values than in the absence of the drugs. In which case, it can be concluded that they are H2 antagonists because they are the ones that inhibit the action of histamine at H2 receptors. Through the action of H2 receptors, histamine is able to secrete gastric juice.
4. The experimental protocol for this practical involved determining two log concentration – response curves, the first one in the absence of the unknown antagonist and the second one in its presence. Could this experimental protocol lead to any error and, if so, and how might it be avoided (e.g. by modifying the protocol)?
Paradoxical potentiating effects: Very low concentrations of aacetycholine sometimes produce a potentiation instead of antagonism and this would interfere with the measurements. More attention should be given when measuring the concentration to ensure that it is
...Download file to see next pagesRead More
Cite this document
(“Analysis of data from the practical class (guinea-pig ileum Lab Report”, n.d.)
Retrieved from https://studentshare.org/biology/1681340-analysis-of-data-from-the-practical-class-guinea-pig-ileum-experiments
(Analysis of Data from the Practical Class (guinea-Pig Ileum Lab Report)
“Analysis of Data from the Practical Class (guinea-Pig Ileum Lab Report”, n.d. https://studentshare.org/biology/1681340-analysis-of-data-from-the-practical-class-guinea-pig-ileum-experiments.
In actual sense, the recipient will have the same combination of genes that are used in the plasmid. this is a type of combining genes from the donor to the recipient, in making a strong production. For instance, during the 1970’s researchers had started the research on how genes could be combined from a donor to the recipient.
Experimental Procedure: You will need to know whether you have been given basic copper carbonate or copper carbonate and accordingly you will have to carry out the molar calculations for getting the volume of the gas at STP (Practical Investigations, n.d.).
Hear sample was heated at elevated temperature leads to evaporation of water molecule from salt and any difference in weight of sample before heating and after heating gives idea of water contain. Following are the steps where once can make errors in measurement so enough care should be taken to avoid it.
uestionnaire was used to collect this data since it yield more information as opposed to interviews and is used as the most common data collection tool (Velicer et al. 1990). The questionnaire had parts on attitude of the participant towards smoking; whether pleasant to smoke,
The collected data was analyzed using SPSS and the results indicated that the confidence to stop smoking was very high as age grew and that females were more confident to stop smoking than males. Poland
Salmonella typhimurium is a leading cause of gastroenteritis in humans
This experiment tested the histidine requirements of the auxotrophic mutant strains and their spontaneous reversion rate. The experiments were carried using either wild
Department of Health and Human Services Food and Drug Administration Center for Drug Evaluation and Research (CDER), 2013). These factors are affected by the chemical composition of the drug forming ingredients. In this experiment, paracetamol tablets made of different
Data issues to the collected data, cleaning and analysis results are discussed. SPSS software was used for analysis.
Missing data was the most prevalent issue in the data set (Tan, Steinbach, & Kumar, 2006). All data for one
2 Pages(500 words)Lab Report
GOT A TRICKY QUESTION? RECEIVE AN ANSWER FROM STUDENTS LIKE YOU!
Let us find you another Lab Report on topic Analysis of data from the practical class (guinea-pig ileum experiments) for FREE!