Anlysis in Biochmeistry - Lab Report Example

Summary

2. The regeneration system contains Glucose-6-phosphate and Glucose-6-phosphate dehydrogenase. What is the purpose for including these chemicals. Is this system of clinical relevance explain and give examples where relevant.
Ans: The chemicals Glucose-6-phosphate and Glucose-6-phosphate dehydrogenase is used for the reaction in which glucose-6-phosphate produce NADPH using Glucose-6-phosphate dehydrogenase which can be used to assay the enzyme by the presence of fluorescence.
…

Download full paper File format: .doc, available for editing

GRAB THE BEST PAPER98.1% of users find it useful

Read Text Preview

Subject: Miscellaneous
Type: Lab Report
Level: College
Pages: 4 (1000 words)
Downloads: 2
Author: carmenrussel

Extract of sample "Anlysis in Biochmeistry"

Download file to see previous pages

The risk can be minimsed by using reliable controls for background fluorescence check as is used in the current study. The average percentage inhibition of the cytochrome P450 3A4 by ketaconazole was determined using a fluorescence based enzyme assay. The readings were obtained as per procedure and is recorded and calculated in Tables 1, 2, 3 and 4. Ketaconazole samples in varying concentrations showed lower fluorescence than the sample without the test compound indicating the inhibitory activity of the drug on the enzyme cytochrome P450.

Saple with 10 M concentration showed the least fluorescence and therefore the highest % inhibition of 95.274.7 and even a lower concentration of 0.1 M also shows a percentage inhibition of about 75.616.12. The fluorescence reading shows the inhibition of the enzyme to be directly proportional to the concentration of the test compound ketaconazole. The present study a fluorescence based assay to illustrate and evaluate the interaction of ketaconazole with the liver cytochrome P450 A4 using microsomes prepared from insect cells that express both human P450 isozyme and rabbit NADPH-P450 reductase and a substrate th

...Download file to see next pages Read More

CHECK THESE SAMPLES OF Anlysis in Biochmeistry

Measurement of Lactate Dehydrogenase Isoenzymes

Cellular signaling is a fascinating process, where protein receptors, transducers, accelerators, inhibitors, and many other substances interact to produce a cellular response.... Enzymes play a critical role in cellular functioning, and a single enzyme deficiency may cause our cells to fail.... hellip; With a tremendous advance in medical and laboratory analysis during the past years, researchers have been able to find information from the measurement of enzymes....

6 Pages (1500 words) Essay

Conventional PCR using agarose gel electrophoresis detection

Conventional techniques used to detect mycoplasma involve culturing samples on selective media, which needs at least a week to obtain the results (HD… The reason to add gel in this method is to either quantify the DNA or separate the particular band of DNA.... With the addition of edhidium bromide, DNA becomes visible....

2 Pages (500 words) Essay

The Cellular Process

The Michaelis Menten plot usually consists of a plot for slope against the concentration of the substrate, thereby acting as the basis for procedure followed in the calculating Km.... Consequently, in this case, there is need to plot the absorbance of the solution vs time for the… The plots gives a straight line which helps in determining the slope for each....

1 Pages (250 words) Essay

The physiology of anhydrobiosis

I do not wish to argue with you - however, I do encourage you to carefully re-read the entire analysis with the understanding that a critical view of anhydrobiosis is presented; especially in terms of its relevance to medical science, evolution, and the way in which future… Naturally, as it is necessary to be descriptive in order to introduce these arguments, there is a degree of descriptive emphasis within the paper....

6 Pages (1500 words) Essay

Testing of biomolecules

This report compiles various methods that have been developed in the testing of biomolecules and depict the importance of each in biochemistry.... The study looks at carbohydrates-three tests including the test for reducing sugars, test for non-reducing sugars, proteins.... hellip; The conclusion from this study states that establishing the presence of various biomolecules serves as the bedrock of successive analyses which include the way they work in the body....

5 Pages (1250 words) Lab Report

Spectrophotometer & Immunochemistry

The paper "Spectrophotometer & Immunochemistry" states that there are different versions of a spectrophotometer, and on the other hand, there are different examples of immunoassays, such as radioimmunoassay, fluorescence immunoassay, etc (Geddes, pp.... 27-38, 2008).... hellip; It is quite essential to state that the paper discussed some of the significant aspects of two specific techniques: Spectrophotometry and Immunoassay (Immunochemistry)....

8 Pages (2000 words) Coursework

Your Future Career: Data, Information, Hardware, and Software

This essay discusses importance future career: data, information, hardware, and software.... For effective use of the writer laptop at home, I would have to acquire internet support systems including wireless home networking equipment.... nbsp;The writer aspires to be a research scientist in the future....

2 Pages (500 words) Assignment

Regulation of Flavonoid Biosynthesis During Tomato Fruit Ripening

From the paper "Regulation of Flavonoid Biosynthesis During Tomato Fruit Ripening", flavonoids are polyphenolic compounds that are ubiquitous in nature and are categorized, according to chemical structure, into flavonols, flavones, flavanones, isoflavones, catechins, anthocyanidins, and chalcones....

7 Pages (1750 words) Research Paper