Anlysis in Biochmeistry - Lab Report Example

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Summary

2. The regeneration system contains Glucose-6-phosphate and Glucose-6-phosphate dehydrogenase. What is the purpose for including these chemicals. Is this system of clinical relevance explain and give examples where relevant.
Ans: The chemicals Glucose-6-phosphate and Glucose-6-phosphate dehydrogenase is used for the reaction in which glucose-6-phosphate produce NADPH using Glucose-6-phosphate dehydrogenase which can be used to assay the enzyme by the presence of fluorescence.
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Subject: Miscellaneous
Type: Lab Report
Level: College
Pages: 4 (1000 words)
Downloads: 2
Author: carmenrussel

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The risk can be minimsed by using reliable controls for background fluorescence check as is used in the current study. The average percentage inhibition of the cytochrome P450 3A4 by ketaconazole was determined using a fluorescence based enzyme assay. The readings were obtained as per procedure and is recorded and calculated in Tables 1, 2, 3 and 4. Ketaconazole samples in varying concentrations showed lower fluorescence than the sample without the test compound indicating the inhibitory activity of the drug on the enzyme cytochrome P450.

Saple with 10 M concentration showed the least fluorescence and therefore the highest % inhibition of 95.274.7 and even a lower concentration of 0.1 M also shows a percentage inhibition of about 75.616.12. The fluorescence reading shows the inhibition of the enzyme to be directly proportional to the concentration of the test compound ketaconazole. The present study a fluorescence based assay to illustrate and evaluate the interaction of ketaconazole with the liver cytochrome P450 A4 using microsomes prepared from insect cells that express both human P450 isozyme and rabbit NADPH-P450 reductase and a substrate th

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