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Cellular Process - Essay Example

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The Michaelis Menten plot usually consists of a plot for slope against the concentration of the substrate, thereby acting as the basis for procedure followed in the calculating Km. Consequently, in this case, there is need to plot the absorbance of the solution vs time for the…
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Cellular Process
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Download file to see previous pages Another important step is plotting the slope, which is the concentration rate, against the substrate concentration.
At maximum concentration of substrate, both the Vmax for the inhibited reaction and for the unhibited reaction should always be equal (Ochs, 2014, p. 56). This brought about my surprise for the observation, which indicated different Vmax for the two reactions. This issue may have arisen from the action of the inhibitor. The low Vmax for the inhibited reaction means that more inhibitor I was supplied. The presence of an inhibitor slows the rate of binding of the enzyme to substrate leading to low turnover number, which is the Kcat. From the equation below, low turnover number leads to low Vmax in comparison to the unhibited reaction (Beard & Qian, 2005, p. 88).
Consequently, solving this issue will require for the reduction of competitive  inhibitor I in order to meet a turnover number equal to that of uninhibited reaction, at maximum concentration (Panesar et al, 2008, p. 78).
Beard, D. A., & Qian, H. (2008). Chemical biophysics quantitative analysis of cellular systems. Cambridge, Cambridge University Press. http://proxy2.hec.ca/login?url=http://library.books24x7.com/library.asp?B̂&bookid=26546.
Berger, M. P. F., & Wong, w.-k. (2009). An Introduction to Optimal Designs for Social and Biomedical Research. Chichester, John Wiley & Sons. ...Download file to see next pagesRead More
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