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Spectrophotometer & Immunochemistry - Coursework Example

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The paper "Spectrophotometer & Immunochemistry" states that there are different versions of a spectrophotometer, and on the other hand, there are different examples of immunoassays, such as radioimmunoassay, fluorescence immunoassay, etc (Geddes, pp. 27-38, 2008)…
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Spectrophotometer & Immunochemistry
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Download file to see previous pages In order to understand the mechanics of spectrophotometry, it is very imperative to discuss the underlying fundamentals associated with this technique. In physics, the second law of thermodynamics has set a condition for organisms to maintain their existence: utilization of the energy. In other words, there is a rule of unvarying supply of energy that enables organisms to continue their lives. Studies (Walker, pp. 50-59, 2008) have indicated that although there are many sources of energy, however, Sun is the ultimate source for all organisms. In this case, pigments become the important components that are responsible for causing the ultimate energy of the sun in the form of molecules that convert into chemical energy through photosynthesis.
Scientists (Gaw & Murphy, pp. 25-27, 2004) have proved that light is made of wavelengths of different modes that cause human eyes to see different colors due to reflection of light at unlike wavelengths. In other words, pigment molecules respond to light ways in diverse ways depending on the structures of these molecules. When a human looks at an object, the eyes process the wavelength of light qualitatively while remaining incapable of processing the quantity of light. For this reason, scientists were successful in creating an electronic instrument that enables the experts to arrange an assessment of light’s reflection and absorbance qualitatively, as well as quantitatively. Although it is a creation of physics, however, it is an observation that a broad range of fields has been beneficial of this capability of a spectrophotometer, such as biochemistry, molecular biology, etc.
In particular, this technique uses an identified wavelength to shine and pass the light ways through an object. Presence of a particular pigment for absorbing light of the specific wavelength in the sample will result in passage of less amount of light. During this process, experts use the light meter to assess the quantity of light that is able to pass through the object. ...Download file to see next pages Read More
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