StudentShare
Contact Us
Sign In / Sign Up for FREE
Search
Go to advanced search...
Nobody downloaded yet

Immunological Basis of Disease - Essay Example

Comments (0) Cite this document
Summary
The "Immunological Basis of Disease" paper analizes such researches as "Mast cells promote airway smooth muscle cell differentiation via autocrine up-regulation of TGF-β1" by Woodman, L., Siddiqui, S., Cruse, G., Sutcliffe, A., Saunders, R., Kaur, D., et al…
Download full paper File format: .doc, available for editing
GRAB THE BEST PAPER98.2% of users find it useful
Immunological Basis of Disease
Read Text Preview

Extract of sample "Immunological Basis of Disease"

Download file to see previous pages a. Comparison of bronchial tissues from asthmatic and non-asthmatic patients by bronchial examination and biopsy. The immunohistochemistry of the proximal airway of bronchial biopsies was conducted by staining them with monoclonal antibodies against smooth muscle actin, and tryptase for mast cells, followed by image analysis.

b. Measuring the effect of the coculturing of mast cells and ASM on the production of smooth muscle actin and TGF-β. Pure cultures of human lung mast cells and airway smooth muscle were produced. ASM cells were labeled with mABs for smooth muscle actin (SMA). ASM cells were cultured with human mast cells, and mast cells that have been sensitized with protease inhibitor and lactoferrin (to inhibit the action of tryptase, a protease produced by mast cells). Under these coculture conditions, the production of smooth muscle actin protein and gene expression were determined periodically for one week using immunofluorescence, flow cytometry, and quantitative real-time PCR. Cell-free supernatants of the cocultures were analyzed for the production of TGF-β1 and TGF-β2 using ELISA. TGF-β ( transforming growth factor-beta) is a protein that controls cell proliferation and differentiation

c. The effect of the addition of recombinant TGF-β on ASM proliferation and contractile activity of ASM pure cultures was also measured by examining SMA protein expression by flow cytometry, and ASM contractile activity by collagen gel analysis.

3. Choose ONE immune-based method used in the paper and, with reference to the paper and the literature, describe this method including its underlying principles. (Use diagrams where appropriate). (No more than 500 words excluding diagram(s)) (30%).

An immune-based method used in the paper was ELISA or enzyme-linked immunosorbent assay. In the paper being reviewed, ELISA was used to quantify the expression of TGF-β1 and TGF-β2 in supernates of ASM and mast cell cultures. ...Download file to see next pages Read More
Cite this document
  • APA
  • MLA
  • CHICAGO
(Immunological Basis of Disease Literature review, n.d.)
Immunological Basis of Disease Literature review. https://studentshare.org/medical-science/1729247-interpretation-of-a-research-paper-immunological-basis-of-disease-coursework
(Immunological Basis of Disease Literature Review)
Immunological Basis of Disease Literature Review. https://studentshare.org/medical-science/1729247-interpretation-of-a-research-paper-immunological-basis-of-disease-coursework.
“Immunological Basis of Disease Literature Review”. https://studentshare.org/medical-science/1729247-interpretation-of-a-research-paper-immunological-basis-of-disease-coursework.
  • Cited: 0 times
Comments (0)
Click to create a comment or rate a document

CHECK THESE SAMPLES OF Immunological Basis of Disease

Neurophysiological Basis of Amnesia

...Immunological Basis of Disease... Neurophysiological Basis of Amnesia Within the spectrum of memory and cognitive disorders, there can be both organic and psychological causes. The lines between the two can blur easily. There are dissociative disorders in which medical testing is required to rule out neurophysiological factors that can mimic the effects of deeper, organic dysfunction. Key distinguishing factors are coming to light due to new research, and the post-mortem study of affected brains. While simple brain trauma or disease-states can cause the loss of memory, there are organic/medical conditions that may also impede new memories. The classic form of 'soap-opera' amnesia seems to close off personal information, allowing a fresh start ...
12 Pages (3000 words) Essay

Asthma. Pathologic basis of disease

...Immunological Basis of Disease... Immunological Basis of Disease... ASTHMA Institute Asthma Asthma is a chronic inflammatory disease of air passage. Asthma causes recurring episodes of breathlessness, chest tightness, wheezing and cough. The episodes mostly occur at night or early in the morning. Due to the inflammation of the airways, the airways become more prone to constriction on stimulus. The constriction of the airways on stimulus limits the airflow to lungs and due to scarcity of air these symptoms occur. The patient suffering from asthma experience attacks of dyspnea, coughing and wheezing. Dyspnea is the shortness of breath or air hunger. The bronchospasm is the major cause of dyspnea. Asthma can be divided into different groups on the...
5 Pages (1250 words) Research Paper

Molecular Basis of Disease

...Immunological Basis of Disease... Immunological Basis of Disease... Histology and molecular diagnostics play a critical role in the characterization of diseases in their most basic s and establishing the pathology of diseases. These techniques have been used in the characterization of various types of malignant cells in human beings and as a result act as necessary adjuvant in the study of oncology (Coleman, 2009, p.89). Cancer are abnormal human cells characterized by “unregulated growth, impaired cellular differentiation, invasiveness and metastatic potential” (Coleman, The Molecular Basis of Human Cancer, 2002, p.3). These characteristics of cancer cells have been used to clinically distinguish cancer cells from normal cells during diagnosis...
8 Pages (2000 words) Lab Report

Molecular Basis of Disease

...Immunological Basis of Disease... Immunological Basis of Disease... PERNICIOUS ANEMIA Introduction Pernicious anemia is a disease caused by the loss of the parietal cells of the gastric mucosa. These cells produce an essential protein known as intrinsic factor. The intrinsic factor helps in vitamin B12 absorption. Vitamin B12 is normally absorbed in the walls of the ileum. The vitamin helps in maturation of red blood cells. According to Doctor Mellissa Conrad (Conrad, 1996), the large multinucleated cells known as megaloblasts circulate in the blood cells but do not act like blood cells. People lacking the absorption ability of vitamin B12 remain with the disease for the rest of their lives. Parietal cells found in the gastric cells are...
6 Pages (1500 words) Lab Report

Disease

...Immunological Basis of Disease... Immunological Basis of Disease... Drivers of emergence of viral diseases in humans Introduction The past few decades have witnessed anincreased interest in the upsurge in the number of viruses causing unanticipated sicknesses and spates among people, livestock and wildlife. In many instances, the outbreaks have severely strained both the local and national resources during periods when health care expenditure in the economically developed states has been restrained. Emerging illness is a phrase that has been utilized with augmenting occurrence to delineate the appearance of an unidentified contagion, or a beforehand identified contagion that expanded to a new ecological niche or a geographical area (Howard &...
9 Pages (2250 words) Essay

Disease

...Immunological Basis of Disease... Immunological Basis of Disease... reflux disease (GERD) Introduction Gastro esophageal reflux disease also known as acid reflux disease or just asGERD is a lifestyle disease and always a long-lasting symptom of mucosal damage. The extensive damage of the mucus lining of the esophagus results from production of an excessive acid by the stomach most of which move backward to the esophagus. Whenever such movements of stomach acids likes the hydrochloric acid that help in the digestion of food leaks from the stomach back to the esophagus, the mucus lining of the wall prevent them from corroding the esophagus. Additionally, such backward movement of stomach and intestinal acids are rare since the digestion system has...
4 Pages (1000 words) Essay

Basis of Finance

...Immunological Basis of Disease... safeguards do banks need to have in place to ensure that your money there is safe? People believe that banks are the safest places to keep moneybut this is not true because hitches occur quite often. This is caused by inability on the side of banking institutions to meet their financial obligations. If this happens, a bank is liquidated hence exposing people to risk of losing their monies. Banks should have insurance cover to secure their institutions and more so customers’ deposits. Insurance will compensate it in case of any loss. Robbers often break into banks and go away with huge sums of money belonging to account holders. This may make banks to go under since they will no longer have funds to meet the...
1 Pages (250 words) Assignment

Robbins and Cotran Pathologic Basis of Disease

...Immunological Basis of Disease... Immunological Basis of Disease... HYPERTHYROIDISM The thyroid gland is an endocrine gland which is present in the neck. It lies in front of the trachea and immediately below the larynx. The basic function of the thyroid gland is the secretion of the thyroid hormones which are triiodothyronine T3 and thyroxine T4. Thyroid stimulation hormone TSH is a hormone of the anterior pituitary gland which binds to specific receptors on the thyroid gland and triggers the release of the thyroid hormones. Increased amount of the thyroid hormones in the blood is referred to as hyperthyroidism. This can result due to many causes which include over activity of the thyroid gland a...
1 Pages (250 words) Essay

Disease

...Immunological Basis of Disease... syndrome Introduction Down syndrome is a condition resulting from abnormal cell division that presents with an extra genetic material from chromosome 21. The implication of Down syndrome varies in severity but causes a lifelong disability in the intellectual system as well as in developmental delays. Down syndrome is the most common genetic disorder and has an epidemiological prevalence rate of one in every one thousand births. Etiology of Down syndrome The cells of the human contain 23 pairs of chromosomes, each pair of the chromosomes are inherited from each parent. During cell division of each pair of the chromosomes from either parent, a genetic malfunction can occur resulting in the development of an ex...
1 Pages (250 words) Essay

Immunological Techniques Lab Assignment

...Immunological Basis of Disease... Draw the results obtained from each of the three procedures. (what i should have gotten) Electrophoresis: Radial immunodiffusion: Ouchterlony: Plate 2: Plate 3: 2.) Explain the origin of each of the precipitin arcs in each of the drawings. Immunoelectrophoresis: We obtain arcs of precipitin showing the existence of Ag-Ab reactions. Between whole serum and anti-whole serum we obtain more arcs showing more cross linking. Radial Immunodiffusion: We obtain precipitin rings of various sized around the antigen wells as a result of radial diffusion of the antigens. The ring diameters can be used to quantify the concentration of the antigens. Ouchterlony: In the 1st petri dish we obtain “pattern of iden...
2 Pages (500 words) Lab Report
sponsored ads
We use cookies to create the best experience for you. Keep on browsing if you are OK with that, or find out how to manage cookies.

Let us find you another Essay on topic Immunological Basis of Disease for FREE!

Contact Us