An Absorbance of Wavelength of Dye - Essay Example

In order to ensure accuracy and precision, it was necessary to re-calibrate the spectrophotometer three times at every new wavelength set. Recalibration of the spectrophotometer was done by filling the cuvettes with deionized water and altering the wavelength to 470 nm, 565 nm, or 635 nm. Before starting the spectrophotometer, ensure that the lid is closed. Results were obtained by running each sample three times at every new wavelength set. The new wavelength for the second part of the experiment was then obtained by comparing the recorded data. The results indicated that the blue dye had an absorbance of 0.211 for the 565 nm wavelength that correlates to the green wavelength that was the highest when compared to the red and green. After choosing the dye, 10 different stock solutions with concentrations that range from 0% to 100% by an increment of 10 dilutions were made. The concentration of the standard solution (the 100% concentration of dye) was reduced by diluting with deionized water, in a graduated cylinder, to make the dilutions that depended on the calculations made. The dilutions were then placed into the spectrophotometer to obtain their percent transmittance. The data was then recorded into a table.