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Fluorescence and Pharmaceuticals - Essay Example

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Download file "Fluorescence and Pharmaceuticals" to see previous pages... fluorophore through the absorption of light energy, a transient excited lifetime with some

loss of energy, and return of the fluorophore to its ground state accompanied by the

emission of light. Due to the energy lost during the transient excited lifetime, the light

energy emitted is always of a longer wavelength than the light energy absorbed,
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and that

is used to study different life processes (Molecular expressions).

Today, there is an increased use of these techniques encouraged mainly by labeled

antibody techniques (Coons and Kaplan, 1950) and by application of fluorescent dyes as

tracers in histochemical techniques. Aminoacridine compounds have special affinity for

nucleic acids; a sensitive fluorescence technique in which acridine orange is used for the

identification of DNA and RNA in mammalian cells (Anderson, Armstrong, and Niven,

1959). Thus using fluorescence techniques and microscopy, the precise location and

dynamics of intracellular components labeled with specific fluorophore designed for the

cell system and the targeted interaction as applied to a pharmaceutical agent. This domain

also, as a result, includes the study of other physicochemical properties of the concerned

molecule, diffusion coefficient, transport characteristics, and above all the interaction

with other biomolecules present. When applied to the field of study of pharmaceuticals

and their effect on cell systems, this can allow one to study the phenomenal response in

fluorescence to localized cellular environmental variables, such as, variation in pH,

viscosity, refractive index, ionic concentrations, membrane potentials, and solvent

polarity in living cell systems and tissue preparations with extraordinarysensitivity...
Anderson, E. S., Armstrong, J. A., And Niven, J. S. F., 1959. 'Observation Of Virus GrowthsWith Aminoacridines.' 9th Symposium Of The Society For General Microbiology, April,1959. Cambridge (University Press).
Medical Research Service, Department Of Veterans Affairs Medical Center,1 And Oregon Health And Science University,2 Portland, Oregon, And Department Of Biochemistry, Mahidol University,Bangkok, Thailand3 ...Download file "Fluorescence and Pharmaceuticals" to see next pagesRead More
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