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Immuno-labelling, fluorescence microscopy and live time-lapse imaging - Lab Report Example

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Immuno-Labelling, Fluorescence and Live Time-Lapse Imaging Name: Institution: Tutor: Course: Date: Abstract This paper seeks to explore the cellular changes in the tracheal and lung cell. The changes are found to be influenced by the presence of proteins in the cell sub-tissues and organelles…
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Immuno-labelling, fluorescence microscopy and live time-lapse imaging
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Download file to see previous pages The background of the alterations in the cell behaviour to include shape and movement is examined in the paper. The paper seeks to explain the significance of the microtubules in the cell, which is to increase the motility of the cells and the affinity of the cells on different reactions as detected in the body and the cell physiologies (Diaspro, 2011. p. 125). The examination of the process of immune labelling in the experiment to detect the presence and effects of the proteins in the cells is explained. The aims and objectives of an experiment basically answer the intent of the experiment. In this case the aims are directed towards the administration and examination of the cell physiology and the detection of the cell proteins and the reaction tests of the antibodies and antigens of the cell. This work explores on the background of the experiment and the purposes of the involvement of the cell physiological processes and the influences they have, such as the proteins, microtubules. The explanations of the experiment’s procedures, the tasks, actions, results and the findings are all covered in the paper. Introduction Immuno-labelling, fluorescence microscopy and live time-lapse imaging involves the cellular biological experiments which aim to detect as well as visualize the structures of the cells while analyzing the cellular changes in the cell and its substructures during a certain period of the body biological processes (Reimer & Kohl, 2008). This experiment involves the study of the immune system dynamics in the human tracheal cells. The involvement of the fluorescent microscopy in the experiment implies the detection of the fluorescent protein in the cell organelles, while observing other defects in the structure of the cell during the experiment. The experiment is not targeted at one result as the process includes diverse measures to analyze different structures in the cells. The process generally involves the analysis of the cell health to entirety (Widdows, Kingdom & Ansari, 2009 pp 735). Immuno labelling is a biological process that involves the detection of antigen to a particular site in a cell, tissue or organ. In any immune labelling reaction the antigen and the antibody reacts within the given organ or tissue or cell in the experiment. The experimentation of the immuno labelling in the cell experiments are meant to reveal information about the cell or the substructures of the cells. In these processes, the antigens can be detected and visualizes using a combination of antigen-specific antibody in the process of detection referred to as tag, which is covalently related to the antibody (Tiwari & Nagai, 2013. pp 497). The immune cytochemistry, which is the process involving the imagery and analysis of the cell biology through fluorescent microscopy, compare the amplification strategies for the primary antibody detection in the cells. The results of the experiment after the detection analysis are used to direct action towards achieving the recommended remedies (Hyatt & Wise, 2001. pp 97). Background of the Experimental Processes The cells of the immune system in the human body explore a wider existence territory than any other cells in the biological systems. The immune-imagery involves t6he visualization of the cells in the native tissues with the intent to detect the changes in the cell biological process of the cell. This process involves the quantification of the immune responses at the cellular level. It is through the process that the ...Download file to see next pagesRead More
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