The researcher of this essay aims to analyze electron microscope, that is a type of microscope which uses a beam of electrons to create an image of the specimen. Additionally, It is an essential component for scientific analysis of a variety of materials…
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The light microscope was invented in the 17th century from the Galilean telescope. Antony van Leeuwenhoek, a Dutchman developed one of the early microscopes which consisted of a powerful convex lens and an adjustable holder for the object being studied. This instrument had a magnifying power of 400x and protozoa, spermatozoa, bacteria and shape of the red blood cells were discovered by Leeuwenhoek (FEI company, 2008). This microscope had only one lens and was called a single microscope. An improvement on this was compound microscope wherein another convex lens was added to magnify the image produced by the first lens. A modern light microscope has a magnification of as high as 1000x and thus enables resolution of objects separated by 0.0002mm (FEI Company, 2008). The resolving power of light microscope had 3 limiting factors: lenses, quality of lenses and the wavelength of light used for illumination. Some improvements in the light microscope were made using these aspects. Blue or ultraviolet light with shorter wavelength gave a small improvement. Further improvement in the resolution was noticed when the specimen and the front of the objective lens were immersed in a medium like oil with high refractive index (FEI Company, 2008). As early as the middle of 19th century, microscopists realized that structures less than half a micrometer could not be resolved with a light microscope. At the same time, researchers had hinted at the possibility of improvement in the resolution of the microscope using electrons rather than light. This is because accelerated electrons behave in a vacuum just like light, they travel in straight lines and have a wavelength which is about 100,000 times smaller than that of light.
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From this paper, it is clear that TEM has a large number of applications in innumerable fields ranging from life sciences to material science. TEM has proved to be a priceless tool for studying the ultrastructure of metals (Egerton 2005, 14). In life sciences, it is used for studying bacteria, viruses, and tissues of plants and animals.
This essay analyzes that spherical aberration also occurs in the Electron Microscopes when electrons passing through the side of the lens are refracted greater than those passing along the axis.2 (Lam, 2009); while Diffractive aberrations are brought about by the deviations from geometrical optics caused by the wave nature of light.
SEM images were used to carry out size distribution analysis of the powder particles using a software package CARNOY. BSE image of the powder particles was taken and EDS was performed to get chemical information about bright particles in the powder sample.
From this paper it is clear that to obtain such a pattern, the selected area aperture is placed in the image plane of the objective lens and used to select only one part of the image. Using projector lenses to focus on electron beams to obtain small spots on the object surface, the diffraction patterns can be obtained.
Different attributes of particles like particle shape, size and size distribution and chemical composition of different particles were determined. SEM analysis shows that there is multimodal distribution of particle size with modes at 25 ?m, 60 ?m and 115 ?
Images in TEM are obtained by focusing an electron beam on the specimen. The electrons are absorbed, transmitted, scattered or backscattered. Depending on the kind of image required by the operator, either the transmitted electrons (called direct beam) or the scattered electrons (called diffracted beam) is selected.
Knowledge of a biomedical laboratory instrument like electron microscope is vital for understanding the reasons why medical health experts can now openly discuss microorganisms or the invisible organisms in nature.
Essentially, electron microscopes are similar to any other microscopes in the sense that it can magnify the size of objects into visible sizes.
The contractor does not want to redesign the circuitry to match that used by Neutron Company despite the fact that the use of their recommended components will raise the price of the contract. Since the contract was awarded because of their significantly low bid, the
The signals derived from the interaction between the sample and the electron reveals information regarding the sample inclusive of texture, material orientation in the sample, crystalline structure, and chemical composition. During most of its applications, data collected over the selected sample surface area is generated as a 2-D image which displays the spatial variations of the properties being investigated.
Research improves individuals’ knowledge about a certain topic or field to supersede the casual outlook or perception about the given field of study. Research serves many functions in academic work. One purpose of research in academic works is to help in in-depth
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